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ENGLISH ABSTRACT
JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
[Analysis of the E gene sequences of three dengue virus type 1 isolates in Guangdong province].
OBJECTIVE: To trace the infection sources of three dengue virus type 1 isolates found in Guangdong province, namely GD05/99, GD14/97 and GD23/95.
METHODS: According to the genomes of dengue virus type 1 S275 strain, a pair of primers was designed for amplification of the structural protein E gene of the isolated dengue virus type 1 strains by reverse transcriptase-PCR. The amplified gene fragment was then cloned into pMD18-T vector and sequenced.
RESULTS: GD14/97 and GD05/99 shared a high nucleotide homology with Cambodia (98% and 99% respectively) and may belong to the same genotype. GD23/95 had a 95% nucleotide homology with A88, higher than that with other strains. GD2395 and A88 may belong to another genotype.
CONCLUSION: The dengue fever outbreak in Guangdong during 1999, 1997, and 1995 may originate from the viruses from different countries.
METHODS: According to the genomes of dengue virus type 1 S275 strain, a pair of primers was designed for amplification of the structural protein E gene of the isolated dengue virus type 1 strains by reverse transcriptase-PCR. The amplified gene fragment was then cloned into pMD18-T vector and sequenced.
RESULTS: GD14/97 and GD05/99 shared a high nucleotide homology with Cambodia (98% and 99% respectively) and may belong to the same genotype. GD23/95 had a 95% nucleotide homology with A88, higher than that with other strains. GD2395 and A88 may belong to another genotype.
CONCLUSION: The dengue fever outbreak in Guangdong during 1999, 1997, and 1995 may originate from the viruses from different countries.
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