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Journal Article
Research Support, U.S. Gov't, P.H.S.
Characterization of phospholipase activity of the Pseudomonas aeruginosa type III cytotoxin, ExoU.
Journal of Bacteriology 2005 Februrary
Recombinant ExoU (rExoU) and yeast extract were used to optimize an in vitro phospholipase assay as a basis for identifying the mechanism for enzyme activation and substrate specificity. Our results support a model in which a eukaryotic protein cofactor or complex facilitates the interaction of rExoU with phospholipid substrates.
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