[Effects of exogenous tumor necrosis factor alpha on the invasiveness of human trophoblasts]

Hui Chen, Zhan-jun Pang, Shi-ling Chen, Fu-qi Xing
Di 1 Jun Yi da Xue Xue Bao, Academic Journal of the First Medical College of PLA 2004, 24 (4): 397-9, 407

OBJECTIVE: To observe the effect of exogenous tumor necrosis factor alpha (TNF-alpha) and its polyclonal antibody (anti-TNF-alpha) on the invasiveness of human trophoblasts in vitro.

METHODS: The effect of exogenous TNF-alpha and anti-TNF-alpha on the expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinases-2 (TIMP-2) in human trophoblastic cells was investigated with reverse transcription (RT)-PCR (RT-PCR).

RESULTS: The trophoblasts cultured in vitro expressed MMP-2 but not TIMP-2. After incubation of the trophoblasts with 5 ng/ml TNF-alpha and TNF-alpha plus anti-TNF-alpha respectively for 48 h, significant elevation of MMP-2 expression was induced, while the reverse occurred following incubation of the cells with 50 ng/ml anti-TNF-alpha. None of the treatments induced TIMP-2 expression.

CONCLUSION: Exogenous TNF-alpha may promote the invasiveness of the trophoblasts, which can be inhibited by anti-TNF-alpha.

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