Use of genome selected repeated sequences increases the sensitivity of PCR detection of Tropheryma whipplei

Florence Fenollar, Pierre-Edouard Fournier, Catherine Robert, Didier Raoult
Journal of Clinical Microbiology 2004, 42 (1): 401-3
The availability of the Tropheryma whipplei genome offers the putative possibility of choosing logical DNA targets. We applied a PCR assay (targeting repeated sequences of T. whipplei) to samples from patients with Whipple's disease and to those from members of a control group. When compared to the results seen with regular PCR, the sensitivity of repeat PCR was significantly enhanced (P = 0.02) without alteration of its specificity.

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