Overproduction of PU.1 in mast cell progenitors: its effect on monocyte- and mast cell-specific gene expression

Chiharu Nishiyama, Makoto Nishiyama, Tomonobu Ito, Shigehiro Masaki, Keiko Maeda, Nobutaka Masuoka, Hisakazu Yamane, Toshio Kitamura, Hideoki Ogawa, Ko Okumura
Biochemical and Biophysical Research Communications 2004 January 16, 313 (3): 516-21
The Ets family transcription factor PU.1 is required for development of various lymphoid and myeloid cell lineages, and regulates the expression of several genes in a cell type-specific manner. Mouse bone marrow-derived hematopoietic progenitor cells are programmed to differentiate into mast cells, when the cells are maintained in the presence of pokeweed mitogen-stimulated spleen-conditioned medium. However, by retroviral introduction of PU.1 cDNA, the progenitor cells expressed MHC class II, CD11b, CD11c, and F4/80, and acquired the ability to stimulate T cells. Furthermore, PU.1-overproducing cells exhibited the morphology, in part, similar to that of monocyte. These results indicate that the mast cell progenitors still have the ability to express monocyte-specific genes by increased expression of PU.1.

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