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Monoclonal antibody purification by affinity chromatography with ligands derived from the screening of peptide combinatory libraries

S A Camperi, N B Iannucci, G J Albanesi, M Oggero Eberhardt, M Etcheverrigaray, A Messeguer, F Albericio, O Cascone
Biotechnology Letters 2003, 25 (18): 1545-8
14571980
The peptide, Ala-Pro-Ala-Arg (APAR), was selected from the screening of a tetrapeptide combinatorial synthetic library as the ligand for affinity purification of an anti-Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) monoclonal antibody (Mab) developed in mouse ascitis. The affinity chromatographic matrix obtained by attachment of APAR to agarose, having a peptide density of 0.5 micromol ml(-1), showed a maximum capacity of 9.1 mg Mab ml(-1) and a dynamic capacity of 3.9 mg Mab ml(-1). A 95% yield of electrophoretically pure anti-GM-CSF was obtained in a single step.

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