JOURNAL ARTICLE

[Role of antisense oligodeoxynucleotide of inducible nitric oxide synthase in the apoptosis and p-p38 MAPK signal transduction in rat neurons after spinal cord injury]

Xin-yu Zhang, Chu-song Zhou, An-min Jin, Ai-lin Guo, Jing Tian
Di 1 Jun Yi da Xue Xue Bao, Academic Journal of the First Medical College of PLA 2003, 23 (6): 578-80, 583
12810381

OBJECTIVE: To study the role of antisense oligodeoxynucleotides (ASODN) of inducible nitric oxide synthase (iNOS) on the apoptosis and p-p38 mitogen-activated protein kinase (p-p38 MAPK) signal transduction of the neurons after spinal cord injury (SCI).

METHODS: Antisense and non-sense oligodeoxynucleotides of iNOS were designed and synthesized, and injected into the subarachnoid space 12 h before the compressive injury was given to the rat spinal cord. Reverse transcription-PCR and flow-cytometry were used to examine the iNOS mRNA expression and the apoptosis of the neurons within the injured spinal cord. The changes of p-p38 MAPK signal transduction pathway were detected by Western blotting.

RESULTS: Obvious increase in iNOS expression and up-regulated p-p38 MAPK were detected after SCI, and neuronal apoptosis was observed in the injured spinal cord. ASODN-iNOS treatment resulted in decreased expression levels of iNOS mRNA and p-p38 MAPK, and the neuronal apoptosis was alleviated.

CONCLUSION: ASODN-iNOS inhibits iNOS expression and neuronal apoptosis following SCI which may be related to the p-p38 MAPK signal transduction pathway.

Full Text Links

Find Full Text Links for this Article

Discussion

You are not logged in. Sign Up or Log In to join the discussion.

Trending Papers

Remove bar
Read by QxMD icon Read
12810381
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"