A simple and rapid fluorescence-based immunoassay for the detection of staphylococcal enterotoxin B

Akbar S Khan, Cheng J Cao, Roy G Thompson, James J Valdes
Molecular and Cellular Probes 2003, 17 (2-3): 125-6
The bioterrorism threat is perceived to be a real challenge to our nation's security. This threat has necessitated the design of better and faster assays for the detection of biothreat agents including staphylococcal enterotoxin B (SEB), a causative agent of food poisoning. This study describes a simple, fast and highly sensitive fluorescence-based immunoassay, in which the antibody is fluorescently-labeled for use in this assay. Use of labeled antibodies resulted in very low level of detection of SEB, 100 pg/well. This method is four times faster than classical and conventional enzyme-linked immunosorbent assay (ELISA).

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