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JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
Med, a cell-surface localized protein regulating a competence transcription factor gene, comK, in Bacillus subtilis.
Bioscience, Biotechnology, and Biochemistry 2002 April
Med was found as a positive regulator for comK, a master regulator for late competence genes. It was found by Western analysis that the ComK level was decreased in a med mutant. Experiments using an alkaline phosphatase fusion with Med and Western analysis of Med were done because a putative lipo-modification signal is found at the N-terminus of Med. The results obtained are consistent with the localization of Med at the cell surface. An implication of the cell-surface localization of Med is discussed in terms of comK regulation.
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