Production of biosurfactant from a new and promising strain of Pseudomonas aeruginosa PA1

L M Santa Anna, G V Sebastian, N Pereira, T L Alves, E P Menezes, D M Freire
Applied Biochemistry and Biotechnology 2001, 91: 459-67
The Pseudomonas aeruginosa PA1 strain, isolated from the water of oil production in Sergipe, Northeast Brazil, was evaluated as a potential rhamnolipid type of biosurfactant producer. The production of biosurfactants was investigated using different carbon sources (n-hexadecane, paraffin oil, glycerol, and babassu oil) and inoculum concentrations (0.0016-0.008 g/L). The best results were obtained with glycerol as the substrate and an initial cell concentration of 0.004 g/L. A C:N ratio of 22.8 led to the greatest production of rhamnolipids (1700 mg/L) and efficiency (1.18 g of rhamnolipid/g of dry wt).

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