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Factors relating to a successful cryopreserved blastocyst transfer program.

OBJECTIVE: To review the authors' experience in a successful frozen blastocyst program.

DESIGN: Retrospective study.

SETTING: University IVF program.

PATIENTS: Women of all ages undergoing 64 frozen blastocyst nondonor thaw cycles.

INTERVENTION(S): Thaw cycles with day 5 or day 6 frozen blastocysts replaced into luteal day 5 endometrium in natural or programmed cycles; cryopreservation of blastocysts by Menezo two-step protocol and Testart slow cool program; thawing by two step thaw protocol.

MAIN OUTCOME MEASURE(S): Implantation, clinical pregnancy, and delivery rates.

RESULT(S): The implantation rate was 16% and was similar with day 5 frozen blastocysts and day 6 frozen blastocysts cycles. The clinical pregnancy rate was 36% and the delivery rate was 27%, with no significant difference between day 5 and 6 blastocysts.

CONCLUSION(S): Blastocyst cryopreservation is a viable option for patients of all ages and complements fresh blastocyst culture and transfer. The presence of good quality blastocysts for freezing on day 5 and day 6 yields comparable results and is critical for the success of thaw cycles.

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