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Comparative Study
Journal Article
Immulite 2000 parathyroid hormone assay: stability of parathyroid hormone in EDTA blood kept at room temperature for 48 h.
Annals of Clinical Biochemistry 2001 September
Parathyroid hormone (PTH) concentrations were compared in serum and EDTA plasma from 36 patients attending a renal stone clinic. Serum PTH concentrations ranged from 0.9 to 10.9 pmol/L, with a mean of 4.6pmol/L. When serum and EDTA plasma results were compared, in samples frozen within 30 min of collection, EDTA plasma results were found to be significantly higher than those in serum (P < 0.0001; Wilcoxon test), with an average increase of 19.5% over the serum result. Results from EDTA-preserved blood left to stand at room temperature for 48 h were on average 14.8% lower than results from the corresponding EDTA plasma samples frozen within 30 min, with highly significant difference (P < 0.0001). Freshly frozen serum and 48h EDTA plasma PTH results were not significantly different. Parathyroid hormone in EDTA-preserved blood is not completely stable, and this could lead to misclassification of results for samples which are not frozen quickly.
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