We have located links that may give you full text access.
ENGLISH ABSTRACT
JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
[Cloning of promoter of banana fruit-specific ACC synthase gene and primary study on its function].
Based on our cDNA sequence of ACC synthase gene expressed specifically in banana fruit, we cloned the 5'-flanking proximal region of 1197 bp and distal region of 1556 bp via walking on unknown region of genomic DNA by an improved adaptor ligation PCR, and created a 2591 bp fragment containing a promoter region of 2505 bp and a transcriptional region of 86 bp. The entire 2.5 kb promoter and 5 different 5' deletion variants were fused to the GUS (beta-glucuronidase) cDNA sequence and introduced into leaf, root and fruit cells of banana plant via particle bombardment separately. The results of transient gene expression assay indicated that the obtained promoter region could direct fruit-specific gene expression. The regulatory region for fruit-specificity was possibly located in the region from -1111 to +1 and a positive regulatory region may locate between nucleotide -1111 to -608.
Full text links
Trending Papers
A Personalized Approach to the Management of Congestion in Acute Heart Failure.Heart International 2023
Potential Mechanisms of the Protective Effects of the Cardiometabolic Drugs Type-2 Sodium-Glucose Transporter Inhibitors and Glucagon-like Peptide-1 Receptor Agonists in Heart Failure.International Journal of Molecular Sciences 2024 Februrary 21
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app