A bacterial artificial chromosome library of Lotus japonicus constructed in an Agrobacterium tumefaciens-transformable vector

A E Men, K Meksem, M A Kassem, D Lohar, J Stiller, D Lightfoot, P M Gresshoff
Molecular Plant-microbe Interactions: MPMI 2001, 14 (3): 422-5
We constructed a BAC library of the model legume Lotus japonicus with a 6-to 7-fold genome coverage. We used vector PCLD04541, which allows direct plant transformation by BACs. The average insert size is 94 kb. Clones were stable in Escherichia coli and Agrobacterium tumefaciens.

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