[Strain of Pseudomonas aeruginosa—producer of bioPAV]

O V Turkovskaia, T V Dmitrieva, A Iu Muratova
Prikladnaia Biokhimiia i Mikrobiologiia 2001, 37 (1): 80-5
A Pseudomonas aeruginosa strain producing an extracellular surfactant (biosurfactant) was isolated. The growth of this strain, referred to as 50.3, on a mineral glycerol-containing medium produces an emulsifying activity (60%) and decreases the surface tension of the culture liquid by a factor of 2.8 (to 25 mN/m). The optimum conditions for its growth and production of biosurfactants: intense aeration, pH 7.0-8.0, and the presence of Mg2+. The optimum biosurfactant properties were achieved when glucose was used as the only source of carbon and energy and NH4Cl was used as a source of nitrogen. The biosurfactant was isolated from the culture liquid by extraction and precipitation.

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