Xinyong Cai, Shu Wang, Lang Hong, Songping Yu, Bin Li, Hong Zeng, Xu Yang, Ping Zhang, Liang Shao
Long non-coding RNA taurine upregulated gene 1 (lncRNA TUG1) has been reported to involve in the processing of cardiac ischemia/reperfusion (I/R) injury following myocardial infarction. Thus, this study further investigates the underlying mechanisms of TUG1 in hypoxia/reoxygenation (H/R)-induced cardiomyocyte injury in vitro. Cell viability, apoptosis, migration and invasion were detected using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry and transwell assay, respectively...
August 19, 2020: Journal of Cardiovascular Pharmacology
Kaihua Zhai, Boyu Liu, Lin Gao
Long non-coding RNA taurine up-regulated gene 1 (TUG1) participates in nervous system diseases, but its function in Parkinson's disease remains unclear. This study explored the function and mechanism of TUG1 in Parkinson's disease (PD). A PD model was constructed using SH-SY5Y cells induced by 1-methyl-4-phenylpyridinium (MPP+) in vitro and mice treated by 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in vivo. The expressions of TUG1, miR-152-3p, phosphatase and tensin homolog (PTEN), tyrosine hydroxylase (TH), Bcl-2, cleaved caspase-3 expressions were determined by quantitative reverse transcription-PCR (qRT-PCR) and Western blotting...
August 18, 2020: Human Gene Therapy
Dingqiao Xu, Shanting Liao, Yan Lv, Junsong Wang, Lingyi Kong
Oxidative stress and the production of intracellular reactive oxygen species (ROS) have been implicated in the pathogenesis of sepsis. In excess, oxidative stress is not deemed an unbalanced biochemical reaction in the critically ill rats, but it is a key pathological factor in driving systemic inflammatory response that can result in multiple organ failure in sepsis. Thus, we aimed to explore whether antioxidant nutrients could reduce or delay the oxidative stress condition of sepsis rats, and then play a prospective role in the oxidative stress condition of critical disease...
June 2, 2020: Journal of Nutritional Biochemistry
Jianyin Long, Daniel L Galvan, Koki Mise, Yashpal S Kanwar, Li Li, Naravat Poungvarin, Paul A Overbeek, Benny H Chang, Farhad R Danesh
Long-noncoding RNAs (lncRNAs) have been shown to play key roles in a variety of biological activities of the cell. However, less is known about how lncRNAs respond to environmental cues, and what transcriptional mechanisms regulate their expression. Studies from our laboratory have shown that the lncRNA Tug1 (taurine upregulated gene 1) is crucial for progression of diabetic kidney disease, a major microvascular complication of diabetes. Using a combination of proximity labeling with the engineered soybean ascorbate peroxidase (APEX2), ChIP-qPCR, biotin-labeled oligo-nucleotides pulldown, and classical promoter luciferase assays in kidney podocytes, we extend our initial observations in the current study, and now provide a detailed analysis on how high glucose milieu down-regulates Tug1 expression in podocytes...
May 28, 2020: Journal of Biological Chemistry
Laura Pineda-Cirera, Judit Cabana-Domínguez, Noelia Benetó, Hector Diez, Concepció Arenas, Bru Cormand, Noèlia Fernàndez-Castillo
The nuclear factor of activated T-cells 5 (NFAT5), also known as tonicity-responsive enhancer-binding protein (TonEBP), is a transcription factor that regulates osmoadaptive response in multiple tissues and is highly expressed in the developing central nervous system. A former study reported that NFAT5 activation through hypertonic stress increases the expression of the dopa decarboxylase enzyme (DDC), also known as aromatic-l-amino-acid decarboxylase (AADC), in human renal proximal tubule cells, leading to an increase of dopamine synthesis...
March 9, 2020: Gene
Hoi Man Ng, Jeff Cheuk Hin Ho, Wenyan Nong, Jerome Ho Lam Hui, Keng Po Lai, Chris Kong Chu Wong
BACKGROUND: Gills of euryhaline fishes possess great physiological and structural plasticity to adapt to large changes in external osmolality and to participate in ion uptake/excretion, which is essential for the re-establishment of fluid and electrolyte homeostasis. The osmoregulatory plasticity of gills provides an excellent model to study the role of microRNAs (miRs) in adaptive osmotic responses. The present study is to characterize an ex-vivo gill filament culture and using omics approach, to decipher the interaction between tonicity-responsive miRs and gene targets, in orchestrating the osmotic stress-induced responses...
March 4, 2020: BMC Genomics
A K Singh, R C Upadhyay, Gulab Chandra, Sudarshan Kumar, D Malakar, S V Singh, M K Singh
The present study sought to evaluate mRNA expression profiles in the cultured dermal fibroblasts of Tharparkar (zebu) and Karan-Fries (zebu, Tharparkar × taurine, Holstein Friesian) cattle in response to heat stress. Bioinformatics' analysis identified temperature-regulated biological processes and pathways. Biological processes overrepresented among the earliest genes induced by temperature stress include regulation of stress responses, protein repair, metabolism, protein transport, cell division, and apoptosis...
February 15, 2020: Cell Stress & Chaperones
Minsu Park, Hyeon Kyeong Choi, Jeung Hee An
Background: We aimed to elucidate the preventive effects of taurine against osteopenia in ovariectomized (OVX) rats and the mechanisms by which taurine regulates osteoblastogenesis in vitro and in vivo. Methods: The effects of the taurine on human osteoblast MG-63 cell differentiation and osteoblastogenesis effect in OVX rat were examined Konkuk University in 2018 by evaluating osteoblast differentiation, and expression of osteoblast-specific factors by western blotting analysis...
November 2019: Iranian Journal of Public Health
Hai-Yu Cao, Dong Li, Yun-Peng Wang, Hui-Xiu Lu, Jing Sun, Hai-Bin Li
We aimed to know the effect of nuclear factor-kappa B (NF-κB) inhibition on the kidney injury of systemic lupus erythematosus (SLE) mice. Pristane-induced SLE mice were treated with pyrrolidine dithiocarbamate (PDTC, 50 or 100 mg/kg), a NF-κB inhibitor. Histopathological changes were observed by hematoxylin & eosin, Masson and periodic schiff-methenamine stainings. Long noncoding RNA Taurine upregulated gene 1 (LncRNA TUG1) was measured by real-time reverse transcription PCR, NF-κB p65 expression by western blotting, levels of inflammatory cytokines, antinuclear antibodies (ANA), and antidouble stranded DNA (anti-dsDNA) by enzyme-linked immunosorbent assay, and the deposition of IgG and C3 by immunofluorescence...
January 13, 2020: Kaohsiung Journal of Medical Sciences
Linlin Zang, Yongqing Song, Fengying Yu, Xiuxia Liu
BACKGROUND: Neonatal pneumonia (NP) has a high fatality rate in neonatal illness. This research investigated the functions of emodin on lipopolysaccharide (LPS)-evoked inflammatory injury in WI-38 cells. METHODS: Cell counting kit-8 (CCK-8) assay and flow cytometry were utilized for examining the impacts of LPS and emodin on viability and apoptosis, respectively. Taurine up-regulated gene 1 (TUG1) level was altered through cell transfection and investigated by reverse transcription quantitative polymerase chain reaction (RT-qPCR)...
January 7, 2020: BioFactors
Qin-Li Wan, Xiaodie Fu, Xiao Meng, Zhenhuan Luo, Wenyu Dai, Jing Yang, Chongyang Wang, Hao Wang, Qinghua Zhou
Hypotaurine, an important sulfur-containing and nonpeptidic amino acid, is a precursor of taurine and an antioxidant. Our previous study indicated that hypotaurine levels are associated with the ageing of Caenorhabditis elegans (C. elegans). However, whether hypotaurine plays a role in the lifespan regulation of C. elegans and the mechanism remains undetermined. Here, we found that hypotaurine enhances oxidative stress resistance and ameliorates ageing in C. elegans. Our results show that hypotaurine regulates a variety of pathways and leads to the upregulation of some age-related genes to extend lifespan...
January 29, 2020: Food & Function
David M Favara, Christos E Zois, Syed Haider, Elisabete Pires, Helen Sheldon, James McCullagh, Alison H Banham, Adrian L Harris
Adhesion G Protein-Coupled Receptor L4 (ADGRL4/ELTD1) is an endothelial cell adhesion G protein-coupled receptor (aGPCR) which regulates physiological and tumour angiogenesis, providing an attractive target for anti-cancer therapeutics. To date, ADGRL4/ELTD1's full role and mechanism of function within endothelial biology remains unknown, as do its ligand(s). In this study, ADGRL4/ELTD1 silencing, using two independent small interfering RNAs (siRNAs), was performed in human umbilical vein endothelial cells (HUVECS) followed by transcriptional profiling, target gene validation, and metabolomics using liquid chromatography-mass spectrometry in order to better characterise ADGRL4/ELTD1's role in endothelial cell biology...
November 25, 2019: Metabolites
Nitin Sharma, Alexander V Fonin, Olesya G Shpironok, Sergey A Silonov, Konstantin K Turoverov, Vladimir N Uversky, Irina M Kuznetsova, Rajanish Giri
Transcriptional regulation is a critical facet of cellular development controlled by numerous transcription factors, among which are E-proteins (E2A, HEB, and E2-2) that play important roles in lymphopoiesis. For example, primary hematopoietic cells immortalisation is promoted by interaction of the conserved PCET motif consisting of the Leu-X-X-Leu-Leu (LXXLL) and Leu-Asp-Phe-Ser (LDFS) sequences of the transactivation domains (AD1) of E-proteins with the KIX domain of CBP/p300 transcriptional co-activators...
November 13, 2019: International Journal of Biological Macromolecules
Hongjin Shao, Dianbo Dong, Feng Shao
Introduction: Taurine up-regulated 1 (TUG1) was reported to be over-expressed and involved in various human malignancies. However, its expression status and mechanistic importance in colorectal cancer (CRC) were yet to be defined. Methods: Relative expressions of TUG1, miR-153-1 and Kruppel-like factor 4 (KLF4) were analyzed by real-time PCR. The potential influences of TUG1-proficiency and miR-153-1-deficiency on cell proliferation, migration and viability were determined by colony formation, wound healing and CCK-8 assays, respectively...
2019: Cancer Management and Research
Kunkun Sheng, Yan Li
The lncRNA taurine-upregulated gene 1 (TUG1) is known to serve a role as an oncogene in the development of a number of human malignancies. However, the functionality of TUG1 in osteosarcoma remains poorly characterized. Therefore, the aim of the present study was to explore the role of TUG1 in osteosarcoma. TUG1 expression in tumor tissues, adjacent healthy tissues and plasma from 40 osteosarcoma patients and 40 healthy controls was detected using reverse transcription-quantitative PCR. Receiver operating characteristic curves were used to analyze the diagnostic value of TUG1 for osteosarcoma while the prognostic value of TUG1 for osteosarcoma was analyzed using the Kaplan-Meier method...
October 2019: Experimental and Therapeutic Medicine
Y Wu, W-X Zhang, F Zuo, G-W Zhang
The yattle (dzo) is an interspecific hybrid of the taurine cattle (Bos taurus) and the domestic yak (Bos grunniens). F1 hybrid yattle bulls are sterile due to spermatogenic arrest and have misregulation of spermatogenesis genes in the testes. However, the expression pattern of Y chromosome-linked genes in cattle, yaks and yattle testes is still unknown. In this study, we analyzed the mRNA expression pattern of 10 genes known to be present as single copies in the X-degenerate region of the bovine male-specific region of the Y chromosome...
September 2, 2019: Animal Genetics
Limei Han, Jingyi Yang, Lei Jing, Ge Li, Longfei Li, Minghui Ji, Xianjia Wu, Chunli Zhang
Taurine plays an important role in improving immunity, regulating cell proliferation and differentiation, apoptosis and so on. Traditional Chinese herb formula (TCHF) is a wealth of medicine materials for diseases control. There are many studies on Chinese herb formula in inducing cell apoptosis, differentiation and improving animal immunity. The factors in phosphatidylinositol 3-kinase/Protein Kinase (PI3K-Akt) signaling pathway are central regulators of normal cells, which integrates extra-cellular signals into cells and activates affects cell activities including cell proliferation, differentiation and apoptosis...
2019: Advances in Experimental Medicine and Biology
In Soon Kang, Chaekyun Kim
Taurine is an abundant sulfur-containing amino acid in myeloid cells. It undergoes halogenation in activated phagocytes and is converted to taurine chloramine (TauCl) and taurine bromamine. Bone homeostasis is mediated by the balance between bone-forming osteoblasts and bone-resorbing osteoclasts. Osteoclasts are bone-resorbing multinucleated cells differentiated from monocyte/macrophage precursor cells in response to receptor activator of NF-κB ligand (RANKL). In this study, we investigated the effect of TauCl on RANKL-induced osteoclastogenesis from RAW 264...
2019: Advances in Experimental Medicine and Biology
Yunsheng Li, Dan Zheng, Liutong Pan, Yuanting Dai, Shasha Cai, Lijin Zhao, HuiPing Zhu
Renal cell carcinoma (RCC) is one of the top ten deadly malignancies in the world. The long non-coding RNA taurine up-regulated gene 1 (TUG1) is a transcript that is up-regulated by taurine. There is ample evidence that TUG1 plays a crucial role in the progression of various cancers. This study aimed to investigate the role of TUG1 in RCC and its underlying molecular mechanisms. In the current study, knockdown of TUG1 by shRNA (sh-TUG1) significantly inhibited proliferation, invasion, migration and EMT processes of ACHN cells and OS-RC-2 cells, and induced apoptosis...
July 13, 2019: European Journal of Pharmacology
Zhulei Sun, Gui Huang, Hepeng Cheng
Background: Taurine-upregulated gene 1 ( TUG1 ) has been documented to be implicated in carcinogenesis and chemoresistance in solid tumors. Here, we explored the biological role and regulatory mechanism of TUG1 in progression and chemoresistance of urothelial carcinoma of the bladder (UCB). Methods: Nuclear factor-erythroid 2 ( NF-E2 ) -related factor 2 ( Nrf2 ) mRNA and TUG1 expression was determined by quantitative reverse transcription polymerase chain reaction...
2019: Cancer Management and Research
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