Shawn French, Amelia Bing Ya Guo, Michael J Ellis, Julia P Deisinger, Jarrod W Johnson, Kenneth Rachwalski, Zoë A Piquette, Telmah Lluka, Miranda Zary, Sineli Gamage, Jakob Magolan, Eric D Brown
In the search for much-needed new antibacterial chemical matter, a myriad of compounds have been reported in academic and pharmaceutical screening endeavors. Only a small fraction of these, however, are characterized with respect to mechanism of action (MOA). Here, we describe a pipeline that categorizes transcriptional responses to antibiotics and provides hypotheses for MOA. 3D-printed imaging hardware PFIboxes) profiles responses of Escherichia coli promoter-GFP fusions to more than 100 antibiotics. Notably, metergoline, a semi-synthetic ergot alkaloid, mimics a DNA replication inhibitor...
April 4, 2024: Cell Reports