Jade Glashauser, Carolina Camelo, Manuel Hollmann, Wilko Backer, Thea Jacobs, Jone Isasti Sanchez, Raphael Schleutker, Dominique Förster, Nicola Berns, Veit Riechmann, Stefan Luschnig
Intracellular trafficking of secretory proteins plays key roles in animal development and physiology, but so far, tools for investigating the dynamics of membrane trafficking have been limited to cultured cells. Here, we present a system that enables acute manipulation and real-time visualization of membrane trafficking through the reversible retention of proteins in the endoplasmic reticulum (ER) in living multicellular organisms. By adapting the "retention using selective hooks" (RUSH) approach to Drosophila, we show that trafficking of GPI-linked, secreted, and transmembrane proteins can be controlled with high temporal precision in intact animals and cultured organs...
March 31, 2023: Developmental Cell