Iu A Zolotarev, A K Dadaian, O V Dolotov, V S Kozik, N V Kost, O Iu Sokolov, E M Dorokhova, V K Meshavkin, L S Inozemtseva, M V Gabaeva, L A Andreeva, L Iu Alfeeva, T S Pavlov, K E Badmaeva, S E Badmaeva, Z V Bakaeva, G N Kopylova, G E Samonina, B V Vas'kovskiĭ, I A Grivennikov, A A Zozulia, N F Miasoedov
Biologically active peptides evenly labeled with tritium were used for studying the in vitro and in vivo biodegradation of the peptides. Tritium-labeled peptides with a specific radioactivity of 50-150 Ci/mmol were obtained by high temperature solid phase catalytic isotope exchange (HSCIE) with spillover tritium. The distribution of the isotope label among all amino acid residues of these peptides allows the simultaneous determination of practically all possible products of their enzymatic hydrolysis. The developed analytical method includes extraction of tritium-labeled peptides from organism tissues and chromatographic isolation of individual labeled peptides from the mixture of degradation products...
March 2006: Bioorganicheskaia Khimiia