Mario Alberto Flores-Valdez, Eliza J R Peterson, Michel de Jesús Aceves-Sánchez, Nitin S Baliga, Yasu S Morita, Ian L Sparks, Deepak Kumar Saini, Rahul Yadav, Roland Lang, Dulce Mata-Espinosa, Juan Carlos León-Contreras, Rogelio Hernández-Pando
We have previously reported the transcriptomic and lipidomic profile of the first-generation, hygromycin-resistant (HygR ) version of the BCGΔBCG1419c vaccine candidate, under biofilm conditions. We recently constructed and characterized the efficacy, safety, whole genome sequence, and proteomic profile of a second-generation version of BCGΔBCG1419c, a strain lacking the BCG1419c gene and devoid of antibiotic markers. Here, we compared the antibiotic-less BCGΔBCG1419c with BCG. We assessed their colonial and ultrastructural morphology, biofilm, c-di-GMP production in vitro, as well as their transcriptomic and lipidomic profiles, including their capacity to activate macrophages via Mincle and Myd88...
May 24, 2024: Scientific Reports