Natalie Y Luo, Rachel L Minne, Joseph P Gallant, Gihan S Gunaratne, Jayden L West, Saahil Javeri, Austin J Robertson, Eric W Lake, Jonathan W Engle, Jason C Mixdorf, Eduardo Aluicio-Sarduy, Kwang P Nickel, Reinier Hernandez, Randall J Kimple, Andrew M Baschnagel, Aaron M LeBeau
The Mesenchymal Epithelial Transition (MET) receptor tyrosine kinase is upregulated or mutated in 5% of non-small-cell lung cancer (NSCLC) patients and overexpressed in multiple other cancers. We sought to develop a novel single-domain camelid antibody with high affinity for MET that could be used to deliver conjugated payloads to MET expressing cancers. From a naïve camelid variable-heavy-heavy (VHH) domain phage display library, we identified a VHH clone termed 1E7 that displayed high affinity for human MET and was cross-reactive with MET across multiple species...
March 12, 2024: Bioconjugate Chemistry