W X Qin, F Wan, F Y Sun, P P Zhang, L W Han, Y Huang, H Q Jiang, X T Zhao, M He, Y Ye, W M Cong, M C Wu, L S Zhang, N W Yang, J R Gu
Using a combination of hybridization of PAC to a cDNA library and RACE technique, we isolated a novel cDNA, designated as C17orf25 (Chromosome 17 open reading frame 25, previously named it HC71A), from the deletion region on chromosome 17p13.3. The cDNA encodes a protein of 313 amino acids with a calculated molecular mass of 34.8 kDa. C17orf25 is divided into 10 exons and 9 introns, spanning 23 kb of genomic DNA. Northern blot analysis showed that the mRNA expression of C17orf25 was decreased in hepatocellular carcinoma samples as compared to adjacent noncancerous liver tissues from the same patients...
September 2001: Cell Research