Rong Chen, Yao Wang, Zhenzhen Zhang, Xiaolei Wang, Ya Li, Min Wang, Huifeng Wang, Muchen Dong, Qingjun Zhou, Lingling Yang
PURPOSE: In the present study, we aim to elucidate the underlying molecular mechanism of endoplasmic reticulum (ER) stress induced delayed corneal epithelial wound healing and nerve regeneration. METHODS: Human limbal epithelial cells (HLECs) were treated with thapsigargin to induce excessive ER stress and then RNA sequencing was performed. Immunofluorescence, qPCR, Western blot, and ELISA were used to detect the expression changes of SLIT3 and its receptors ROBO1-4...
May 1, 2024: Investigative Ophthalmology & Visual Science