keyword

actinobacillus pleuropneumoniae

keyword
https://read.qxmd.com/read/32456813/the-actinobacillus-pleuropneumoniae-sulfate-binding-protein-is-required-for-the-acquisition-of-sulfate-and-methionine-but-is-not-essential-for-virulence
#1
Lulu Gao, Li Zhang, Huan Xu, Fan Zhao, Wei Ke, Jie Chen, Jihong Yang, Chao Qi, Jinlin Liu
Actinobacillus pleuropneumoniae is a Gram-negative pathogen that causes porcine pleuropneumonia, an infectious disease responsible for significant losses in the pig industry. Sulfur is an essential nutrient that is widely required by microorganisms; however, the mechanism involved in A. pleuropneumoniae sulfur transport is unknown. In this study, we showed that a periplasmic protein predicted to be involved in sulfur acquisition (sulfate-binding protein (Sbp)), is required for A. pleuropneumoniae growth in chemically defined medium (CDM) containing sulfate or methionine as the sole sulfur sources...
June 2020: Veterinary Microbiology
https://read.qxmd.com/read/32373078/-in-vitro-mixed-biofilm-of-streptococcus-suis-and-actinobacillus-pleuropneumoniae-impacts-antibiotic-susceptibility-and-modulates-virulence-factor-gene-expression
#2
Yang Wang, Shenglong Gong, Xiao Dong, Jinpeng Li, Daniel Grenier, Li Yi
Streptococcus suis ( S. suis ) and Actinobacillus pleuropneumoniae ( A. pleuropneumoniae ) are primary swine pathogens that have been frequently co-isolated from pigs suffering from severe respiratory disease. The purpose of this study was to investigate the biological impacts of the interactions between S. suis and A. pleuropneumoniae . A single- and dual-species culture model was established in vitro via S. suis HA9801 (serotype 2) and A. pleuropneumoniae CVCC265 (serotype 1). The single or mixed biofilms were imaged by confocal laser scanning microscopy...
2020: Frontiers in Microbiology
https://read.qxmd.com/read/32273013/the-antimicrobial-peptide-mpx-kills-actinobacillus-pleuropneumoniae-and-reduces-its-pathogenicity-in-mice
#3
Lei Wang, Xueqin Zhao, Chunling Zhu, Yaya Zhao, Shuangshuang Liu, Xiaojing Xia, Xin Liu, Huihui Zhang, Yanzhao Xu, Bolin Hang, Yawei Sun, Shijun Chen, Jinqing Jiang, Yueyu Bai, Gaiping Zhang, Liancheng Lei, Langford Paul Richard, Hanna Fotina, Jianhe Hu
Actinobacillus pleuropneumoniae is the causative agent of highly contagious and fatal respiratory infections, causing substantial economic losses to the global pig industry. Due to increased antibiotic resistance, there is an urgent need to find new antibiotic alternatives for treating A. pleuropneumoniae infections. MPX is obtained from wasp venom and has a killing effect on various bacteria. This study found that MPX had a good killing effect on A. pleuropneumoniae and that the minimum inhibitory concentration (MIC) was 16 μg/mL...
April 2020: Veterinary Microbiology
https://read.qxmd.com/read/32257367/status-and-gaps-of-research-on-respiratory-disease-pathogens-of-swine-in-africa
#4
REVIEW
P Oba, B Wieland, F N Mwiine, J Erume, E Gertzell, M Jacobson, M M Dione
Over the last two decades, the pig population in Africa has grown rapidly, reflecting the increased adoption of pig production as an important economic activity. Of all species, pigs are likely to constitute a greater share of the growth in the livestock subsector. However, constraints such as respiratory infectious diseases cause significant economic losses to the pig industry worldwide. Compared to industrialized countries, the occurrence and impacts of respiratory diseases on pig production in Africa is under-documented...
2020: Porcine Health Management
https://read.qxmd.com/read/32252773/use-of-meat-juice-and-blood-serum-with-a-miniaturised-protein-microarray-assay-to-develop-a-multi-parameter-igg-screening-test-with-high-sample-throughput-potential-for-slaughtering-pigs
#5
Katharina Loreck, Sylvia Mitrenga, Regina Heinze, Ralf Ehricht, Claudia Engemann, Caroline Lueken, Madeleine Ploetz, Matthias Greiner, Diana Meemken
BACKGROUND: Serological screening of pig herds at the abattoir is considered a potential tool to improve meat inspection procedures and herd health management. Therefore, we previously reported the feasibility of a miniaturised protein microarray as a new serological IgG screening test for zoonotic agents and production diseases in pigs. The present study investigates whether the protein microarray-based assay is applicable for high sample throughput using either blood serum or meat juice...
April 6, 2020: BMC Veterinary Research
https://read.qxmd.com/read/32238774/enhancement-of-apx-toxin-production-in-actinobacillus-pleuropneumoniae-serotypes-1-2-and-5-by-optimizing-culture-conditions
#6
Hoai Thu Dao, Van Tan Do, Quang Lam Truong, Tae-Wook Hahn
Actinobacillus pleuropneumoniae (APP) is a causative agent of porcine pleuropneumonia. Therefore, the development of an effective vaccine for APP is necessary. Here, we optimized the culture medium and conditions to enhance the production yields of Apx toxins in APP serotype 1, 2, and 5 cultures. The use of Mycoplasma Broth Base (PPLO) medium improved both the quantity and quality of the harvested Apx toxins compared with Columbia Broth (CB) medium. Calcium chloride (CaCl2 ) was first demonstrated as a stimulation factor for the production of Apx toxins in APP serotype 2 cultures...
March 27, 2020: Journal of Microbiology and Biotechnology
https://read.qxmd.com/read/32233129/construction-and-immunization-with-double-mutant-%C3%AE-apxibd-%C3%AE-pnp-forms-of-actinobacillus-pleuropneumoniae-serotypes-1-and-5
#7
Hoai Thu Dao, Quang Lam Truong, Van Tan Do, Tae Wook Hahn
Actinobacillus pleuropneumoniae (APP) causes a form of porcine pleuropneumonia that leads to significant economic losses in the swine industry worldwide. The apxIBD gene is responsible for the secretion of the ApxI and ApxII toxins and the pnp gene is responsible for the adaptation of bacteria to cold temperature and a virulence factor. The apxIBD and pnp genes were deleted successfully from APP serotype 1 and 5 by transconjugation and sucrose counter-selection. The APP1Δ apxIBD Δ pnp and APP5Δ apxIBD Δ pnp mutants lost hemolytic activity and could not secrete ApxI and ApxII toxins outside the bacteria because both mutants lost the ApxI- and ApxII-secreting proteins by deletion of the apxIBD gene...
March 2020: Journal of Veterinary Science
https://read.qxmd.com/read/32184239/structural-basis-of-ca-2-dependent-self-processing-activity-of-repeat-in-toxin-proteins
#8
Vojtech Kuban, Pavel Macek, Jozef Hritz, Katerina Nechvatalova, Katerina Nedbalcova, Martin Faldyna, Peter Sebo, Lukas Zidek, Ladislav Bumba
The posttranslational Ca2+ -dependent "clip-and-link" activity of large <u>r</u>epeat-in-<u>t</u>o<u>x</u>in (RTX) proteins starts by Ca2+ -dependent structural rearrangement of a highly conserved self-processing module (SPM). Subsequently, an internal aspartate-proline (Asp-Pro) peptide bond at the N-terminal end of SPM breaks, and the liberated C-terminal aspartyl residue can react with a free ε-amino group of an adjacent lysine residue to form a new isopeptide bond...
March 17, 2020: MBio
https://read.qxmd.com/read/32182745/remotely-sensed-imagery-for-early-detection-of-respiratory-disease-in-pigs-a-pilot-study
#9
Maria Jorquera-Chavez, Sigfredo Fuentes, Frank R Dunshea, Robyn D Warner, Tomas Poblete, Rebecca S Morrison, Ellen C Jongman
Respiratory diseases are a major problem in the pig industry worldwide. Due to the impact of these diseases, the early identification of infected herds is essential. Computer vision technology, using RGB (red, green and blue) and thermal infrared imagery, can assist the early detection of changes in animal physiology related to these and other diseases. This pilot study aimed to identify whether these techniques are a useful tool to detect early changes of eye and ear-base temperature, heart rate and respiration rate in pigs that were challenged with Actinobacillus pleuropneumoniae...
March 9, 2020: Animals: An Open Access Journal From MDPI
https://read.qxmd.com/read/32152227/an-enzyme-based-protocol-for-cell-free-synthesis-of-nature-identical-capsular-oligosaccharides-from-actinobacillus-pleuropneumoniae-serotype-1
#10
Insa Budde, Christa Litschko, Jana I Führing, Rita Gerardy-Schahn, Mario Schubert, Timm Fiebig
Actinobacillus pleuropneumoniae (App) is the etiological agent of acute porcine pneumonia and responsible for severe economic losses worldwide. The capsule polymer of App serotype 1 (App1) consists of [4)-GlcNAc-β(1,6)-Gal-α-1-(PO4 -] repeating units that are O-acetylated at O-6 of the GlcNAc. It is a major virulence factor and was used in previous studies in the successful generation of an experimental glycoconjugate vaccine. However, the application of glycoconjugate vaccines in the animal health sector is limited, presumably because of the high costs associated with harvesting the polymer from pathogen culture...
March 9, 2020: Journal of Biological Chemistry
https://read.qxmd.com/read/32117101/evaluation-of-machine-learning-models-for-predicting-antimicrobial-resistance-of-actinobacillus-pleuropneumoniae-from-whole-genome-sequences
#11
Zhichang Liu, Dun Deng, Huijie Lu, Jian Sun, Luchao Lv, Shuhong Li, Guanghui Peng, Xianyong Ma, Jiazhou Li, Zhenming Li, Ting Rong, Gang Wang
Antimicrobial resistance (AMR) is becoming a huge problem in countries all over the world, and new approaches to identifying strains resistant or susceptible to certain antibiotics are essential in fighting against antibiotic-resistant pathogens. Genotype-based machine learning methods showed great promise as a diagnostic tool, due to the increasing availability of genomic datasets and AST phenotypes. In this article, Support Vector Machine (SVM) and Set Covering Machine (SCM) models were used to learn and predict the resistance of the five drugs (Tetracycline, Ampicillin, Sulfisoxazole, Trimethoprim, and Enrofloxacin)...
2020: Frontiers in Microbiology
https://read.qxmd.com/read/32081115/efficacy-of-bacterial-vaccines-to-prevent-respiratory-disease-in-swine-a-systematic-review-and-network-meta-analysis
#12
Jan M Sargeant, Bhumika Deb, Michele D Bergevin, Katheryn Churchill, Kaitlyn Dawkins, Jennifer Dunn, Dapeng Hu, Carly Moody, Annette M O'Connor, Terri L O'Sullivan, Mark Reist, Chong Wang, Barbara Wilhelm, Charlotte B Winder
A systematic review and network meta-analysis (MA) was conducted to address the question, 'What is the efficacy of bacterial vaccines to prevent respiratory disease in swine?' Four electronic databases and the grey literature were searched to identify clinical trials in healthy swine where at least one intervention arm was a commercially available vaccine for one or more bacterial pathogens associated with respiratory disease in swine, including Mycoplasma hyopneumoniae, Actinobacillus pleuropneumonia, Actinobacillus suis, Bordetella bronchiseptica, Pasteurella multocida, Stretococcus suis, Haemophils parasuis, and Mycoplasma hyorhinis...
December 2019: Animal Health Research Reviews
https://read.qxmd.com/read/32047221/genome-wide-screening-of-lipoproteins-in-actinobacillus-pleuropneumoniae-identifies-three-antigens-that-confer-protection-against-virulent-challenge
#13
Yurou Cao, Lulu Gao, Li Zhang, Lixiang Zhou, Jihong Yang, Lingfu Deng, Jin Zhao, Chao Qi, Jinlin Liu
Actinobacillus pleuropneumoniae is an important veterinary pathogen that causes porcine pleuropneumonia. Lipoproteins of bacterial pathogens play pleiotropic roles in the infection process. In addition, many bacterial lipoproteins are antigenic and immunoprotective. Therefore, characterization of lipoproteins is a promising strategy for identification of novel vaccine candidates or diagnostic markers. We cloned 58 lipoproteins from A. pleuropneumoniae JL03 (serovar 3) and expressed them in Escherichia coli...
February 11, 2020: Scientific Reports
https://read.qxmd.com/read/32015147/basal-level-effects-of-p-ppgpp-in-the-absence-of-branched-chain-amino-acids-in-actinobacillus-pleuropneumoniae
#14
Gang Li, Qian Zhao, Tian Luan, Yangbo Hu, Yueling Zhang, Ting Li, Chunlai Wang, Fang Xie, Wanjiang Zhang, Paul R Langford, Siguo Liu
The (p)ppGpp-mediated stringent response (SR) is a highly conserved regulatory mechanism in bacterial pathogens, enabling adaptation to adverse environments and linked to pathogenesis. Actinobacillus pleuropneumoniae can cause damage to the lungs of pigs, it's only known natural host. Pig lungs are known to have a low concentration of free branched chain amino acids (BCAAs) compared to plasma. We had investigated the role for (p)ppGpp in viability and biofilm formation of A. pleuropneumoniae Now, we sought to determine whether (p)ppGpp was a trigger signal for the SR in A...
February 3, 2020: Journal of Bacteriology
https://read.qxmd.com/read/31902502/comparison-of-metabolic-adaptation-and-biofilm-formation-of-actinobacillus-pleuropneumoniae-field-isolates-from-the-upper-and-lower-respiratory-tract-of-swine-with-respiratory-disease
#15
Doris Aper, Janna Frömbling, Murat Bağcıoğlu, Monika Ehling-Schulz, Isabel Hennig-Pauka
Most outbreaks of disease due to infection with Actinobacillus (A.) pleuropneumoniae are caused by pigs already pre-colonised in tonsillar tissue, where the pathogen is protected from exposure to antibiotic substances administered for treatment. As it has been shown recently under experimental conditions, A. pleuropneumoniae displays host tissue-specific metabolic adaptation. In this study, pairs of A. pleuropneumoniae field isolates were recovered from lung as well as from tonsillar and nasal tissue from 20 pigs suffering from acute clinical signs of pleuropneumonia and showing characteristic pathological lung alterations...
January 2020: Veterinary Microbiology
https://read.qxmd.com/read/31866601/development-of-a-one-run-real-time-pcr-detection-system-for-pathogens-associated-with-porcine-respiratory-diseases
#16
Fujiko Sunaga, Shinobu Tsuchiaka, Mai Kishimoto, Hiroshi Aoki, Mari Kakinoki, Katsumasa Kure, Hanako Okumura, Maho Okumura, Atsushi Okumura, Makoto Nagai, Tsutomu Omatsu, Tetsuya Mizutani
The etiology of Porcine respiratory disease complex is complicated by infections with multiple pathogens, and multiple infections increase the difficulty in identifying the causal pathogen. In this present study, we developed a detection system of microbes from porcine respiratory by using TaqMan real-time PCR (referred to as Dempo-PCR) to screen a broad range of pathogens associated with porcine respiratory diseases in a single run. We selected 17 porcine respiratory pathogens (Actinobacillus pleuropneumoniae, Boldetella bronchiseptica, Haemophilus parasuis, Pasteurella multocida, Pasteurella multocida toxin, Streptococcus suis, Mycoplasma hyopneumoniae, Mycoplasma hyorhinis, Mycoplasma hyosynovie, porcine circovirus 2, pseudorabies virus, porcine cytomegalovirus, swine influenza A virus, porcine reproductive and respiratory virus US strain, EU strain, porcine respiratory coronavirus and porcine hemagglutinating encephalomyelitis virus) as detection targets and designed novel specific primer-probe sets for seven of them...
December 23, 2019: Journal of Veterinary Medical Science
https://read.qxmd.com/read/31835552/rtx-toxins-ambush-immunity-s-first-cellular-responders
#17
REVIEW
Laura C Ristow, Rodney A Welch
The repeats-in-toxin (RTX) family represents a unique class of bacterial exoproteins. The first family members described were toxins from Gram-negative bacterial pathogens; however, additional members included exoproteins with diverse functions. Our review focuses on well-characterized RTX family toxins from Aggregatibacter actinomycetemcomitans (LtxA), Mannheimia haemolytica (LktA), Bordetella pertussis (CyaA), uropathogenic Escherichia coli (HlyA), and Actinobacillus pleuropneumoniae (ApxIIIA), as well as the studies that have honed in on a single host cell receptor for RTX toxin interactions, the β2 integrins...
December 10, 2019: Toxins
https://read.qxmd.com/read/31829171/antibiotic-resistance-in-porcine-pathogenic-bacteria-and-relation-to-antibiotic-usage
#18
I Holmer, C M Salomonsen, S E Jorsal, L B Astrup, V F Jensen, B Borck Høg, K Pedersen
BACKGROUND: Optimal treatment and prudent use of antimicrobials for pigs is imperative to secure animal health and prevent development of critical resistance. An important step in this one-health context is to monitor resistance patterns of important animal pathogens. The aim of this study was to investigate the antimicrobial resistance patterns of five major pathogens in Danish pigs during a period from 2004 to 2017 and elucidate any developments or associations between resistance and usage of antibiotics...
December 11, 2019: BMC Veterinary Research
https://read.qxmd.com/read/31778852/efficacy-of-gamithromycin-injection-administered-intramuscularly-against-bacterial-swine-respiratory-disease
#19
Tianshi Xiao, Yuqi Yang, Yixin Zhang, Ping Cheng, Hongxiao Yu, Ruimeng Liu, Muhammad Ishfaq, Xiuying Zhang
The aim of this study is to evaluate the safety and efficacy of gamithromycin (GAM) for the treatment of naturally occurring bacterial swine respiratory disease (SRD) administered IM. A total of 240 pigs (nine-weeks old) were selected from two sites in Heilongjiang Province of China. The pigs showed severe signs of respiratory disease. Among them, 120 pigs were randomly divided into 4 groups of low dose (3 mg/kg), middle dose (6 mg/kg), high dose (12 mg/kg) GAM IM injection and 2.5 mg/kg tulathromycin (TUL) IM injection (positive control group) for phase II clinical trial to screen effective therapeutic dose...
November 13, 2019: Research in Veterinary Science
https://read.qxmd.com/read/31749775/-in-vitro-and-in-vivo-synergistic-effects-of-florfenicol-and-thiamphenicol-in-combination-against-swine-actinobacillus-pleuropneumoniae-and-pasteurella-multocida
#20
Porjai Rattanapanadda, Hung-Chih Kuo, Thomas W Vickroy, Chi-Hsuan Sung, Tirawat Rairat, Tsai-Lu Lin, Sze-Yu Yeh, Chi-Chung Chou
Potential synergism between florfenicol (FF) and thiamphenicol (TAP) was investigated for in vitro efficacy against Actinobacillus pleuropneumoniae and/or Pasteurella multocida as well as in vivo efficacy in swine. Among isolates of A. pleuropneumoniae ( n = 58) and P. multocida ( n = 79) from pigs in Taiwan that were tested, high percentages showed resistance to FF (52 and 53%, respectively) and TAP (57 and 53%, respectively). Checkerboard microdilution assay indicated that synergism [fractional inhibitory concentration index (FICI) ≤ 0...
2019: Frontiers in Microbiology
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