Gabriela Andrejeva, Sharon Gowan, Gigin Lin, Anne-Christine Lf Wong Te Fong, Elham Shamsaei, Harry G Parkes, James Mui, Florence I Raynaud, Yasmin Asad, Gema Vizcay-Barrena, Joanna Nikitorowicz-Buniak, Melanie Valenti, Louise Howell, Roland A Fleck, Lesley-Ann Martin, Vladimir Kirkin, Martin O Leach, Yuen-Li Chung
Macroautophagy/autophagy can enable cancer cells to withstand cellular stress and maintain bioenergetic homeostasis by sequestering cellular components into newly formed double-membrane vesicles destined for lysosomal degradation, potentially affecting the efficacy of anti-cancer treatments. Using 13 C-labeled choline and 13 C-magnetic resonance spectroscopy and western blotting, we show increased de novo choline phospholipid (ChoPL) production and activation of PCYT1A (phosphate cytidylyltransferase 1, choline, alpha), the rate-limiting enzyme of phosphatidylcholine (PtdCho) synthesis, during autophagy...
June 2020: Autophagy