Daniele Sonaglioni, Valeria Libera, Elpidio Tombari, Judith Peters, Francesca Natali, Caterina Petrillo, Lucia Comez, Simone Capaccioli, Alessandro Paciaroni
Protein dynamics display distinct traits that are linked to their specific biological function. However, the interplay between intrinsic dynamics and the molecular environment on protein stability remains poorly understood. In this study, we investigate, by incoherent neutron scattering, the subnanosecond time scale dynamics of three model proteins: the mesophilic lysozyme, the thermophilic thermolysin, and the intrinsically disordered β-casein. Moreover, we address the influence of water, glycerol, and glucose, which create progressively more viscous matrices around the protein surface...
May 16, 2024: Journal of Physical Chemistry Letters