Hemu Zhuang, Mengzhen Chen, Dongping Hu, Lin Liu, Dandan Wu, Hao Zhang, Zhengan Wang, Shengnan Jiang, Yiyi Chen, Feiteng Zhu, Yueqin Hong, Tailong Lei, Haiping Wang, Lu Sun, Shujuan Ji, Yunsong Yu, Yan Chen
OBJECTIVES: Using a random forest algorithm, we previously found that teicoplanin-associated gene A (tcaA) might play a role in resistance of methicillin-resistant Staphylococcus aureus (MRSA) to β-lactams, which we have investigated further here. METHODS: Representative MRSA strains of prevalent clones were selected to identify the role of tcaA in the MRSA response to β-lactams. tcaA genes were deleted by homologous recombination in the selected MRSA strains, and antibiotic susceptibility tests were applied to evaluate the effect of tcaA on the minimum inhibitory concentrations (MICs) of glycopeptides and β-lactams...
April 29, 2024: International Journal of Antimicrobial Agents