Chao Guo, Qianchun Zhang, Linchun Bao, Hongliang Li, Qingfeng Zheng, Zhenpeng Guo, Yi Chen
As a fast, high-performance and cost-effective separation technique, capillary electrophoresis (CE) is applicable to the screening and diagnosis of diseases such as thalassemia. However, it is often not preferred due to its unrepeatable and/or irreproducible migration times. Herein, we propose a stable version of CE that uses migration charge density instead of the migration time to plot the electropherogram. The peak position is now independent of the applied voltage or current and the capillary geometry and is also insensitive to temperature...
July 2, 2020: Analytical Methods: Advancing Methods and Applications
Catherine Van Der Straeten
BACKGROUND: Total hip arthroplasty (THA) in patients younger than 50 years poses significant challenges including postoperative limitations of activity and higher failure rates. Sub-par outcomes of hip resurfacing arthroplasty (HRA) in registries remain controversial due to multiple confounders. Favourable HRA results in some studies are often regarded as irreproducible. The aim of this study is to analyse HRA outcomes in a large international cohort. PATIENTS AND METHODS: We compiled a database of 11,382 HRA patients ⩽50 years from an international group of 27 experienced HRA centres from 13 countries...
September 9, 2020: Hip International: the Journal of Clinical and Experimental Research on Hip Pathology and Therapy
Lidija Strojnik, Federica Camin, Nives Ogrinc
Natural flavouring materials are in high demand, and a premium price is paid for all-natural flavourings, making them vulnerable to fraud. At present, compound-specific isotope analysis (CSIA) is perhaps the most sophisticated tool for determining flavour authenticity. Despite promising results, the method is not widely used, and the results are limited to the most common volatile organic compounds (VOCs). This paper describes a robust protocol for on-line measurements of δ13 C and δ2 H using HS-SPME coupled with GC-C-IRMS and GC-HTC-IRMS for common fruit VOCs...
November 1, 2020: Talanta
Vera Nyangoma de Ruijter, Paula E Redondo Hasselerharm, Todd Gouin, Albert A Koelmans
In the literature, there is widespread consensus that methods in plastic research need improvement. Current limitations in quality assurance and harmonization prevent progress in our understanding of what the true effects of microplastic in the environment are. Following the recent development of quality assessment methods for studies reporting concentrations in biota and water samples, we propose a method to assess the quality of microplastic effect studies. We reviewed 105 microplastic effect studies with aquatic biota, provided a systematic overview of their characteristics, developed 20 quality criteria in four main criteria categories (particle characterization, experimental design, applicability in risk assessment, and ecological relevance), propose a protocol for future effect studies with particles, and, finally, used all the information to define the weight of evidence with respect to demonstrated effect mechanisms...
August 28, 2020: Environmental Science & Technology
Veronica L Porubsky, Arthur P Goldberg, Anand K Rampadarath, David P Nickerson, Jonathan R Karr, Herbert M Sauro
Like many scientific disciplines, dynamical biochemical modeling is hindered by irreproducible results. This limits the utility of biochemical models by making them difficult to understand, trust, or reuse. We comprehensively list the best practices that biochemical modelers should follow to build reproducible biochemical model artifacts-all data, model descriptions, and custom software used by the model-that can be understood and reused. The best practices provide advice for all steps of a typical biochemical modeling workflow in which a modeler collects data; constructs, trains, simulates, and validates the model; uses the predictions of a model to advance knowledge; and publicly shares the model artifacts...
August 26, 2020: Cell Systems
N M Bruno, S Yuce
The giant magnetocaloric effect was quantified in CoMn1-x Fex Ge (x = 0.085-0.12) nom. at. % polycrystals across the high temperature hexagonal (P63 /mmc) to low temperature orthorhombic (Pnma) phase transition via differential scanning calorimetry (DSC) and multiple (thermo) magnetization measurements. It was found that increasing Fe content led to the decrease of both the martensitic transformation temperature and entropy change ([Formula: see text]) at the point of the phase transition. Moreover, first-time magnetocaloric measurements resulted in irreproducible entropy change versus temperature diagrams, which was attributed to the release of internal pressure in bulk samples that disintegrated into powder upon transformation...
August 26, 2020: Scientific Reports
Ryan Sasada, Michael Weinstein, Aishani Prem, Mingda Jin, Jeff Bhasin
Microbiome studies continue to provide tremendous insight into the importance of microorganism populations to the macroscopic world. High-throughput DNA sequencing technology (i.e., Next-generation Sequencing) has enabled the cost-effective, rapid assessment of microbial populations when combined with bioinformatic tools capable of identifying microbial taxa and calculating the diversity and composition of biological and environmental samples. Ribosomal RNA gene sequencing, where 16S and 18S rRNA gene sequences are used to identify prokaryotic and eukaryotic species, respectively, is one of the most widely used techniques currently employed in microbiome analysis...
August 2020: Journal of Biomolecular Techniques: JBT
Federica Scollo, Carmelo La Rosa
Aβ, IAPP, α-synuclein, and prion proteins belong to the amyloidogenic intrinsically disordered proteins' family; indeed, they lack well defined secondary and tertiary structures. It is generally acknowledged that they are involved, respectively, in Alzheimer's, Type II Diabetes Mellitus, Parkinson's, and Creutzfeldt-Jakob's diseases. The molecular mechanism of toxicity is under intense debate, as many hypotheses concerning the involvement of the amyloid and the toxic oligomers have been proposed. However, the main role is represented by the interplay of protein and the cell membrane...
August 8, 2020: Life
Vicent Giménez-Alventosa, José Damián Segrelles, Germán Moltó, Mar Roca-Sogorb
BACKGROUND:  Scientific publications are meant to exchange knowledge among researchers but the inability to properly reproduce computational experiments limits the quality of scientific research. Furthermore, bibliography shows that irreproducible preclinical research exceeds 50%, which produces a huge waste of resources on nonprofitable research at Life Sciences field. As a consequence, scientific reproducibility is being fostered to promote Open Science through open databases and software tools that are typically deployed on existing computational resources...
August 10, 2020: Methods of Information in Medicine
Yuan-Yuan Zhang, Qi-Liang Chen, Qiong Wang, Shan-Shan Ding, Shu-Nan Li, Shu-Jiao Chen, Xue-Juan Lin, Can-Dong Li, Tetsuya Asakawa
Acupuncture is an ancient therapeutic method based on the theory of Chinese medicine (CM). Traditional acupuncture has many limitations; it is subjective and relies more on the experience of an acupuncturist, and the efficacy is sometimes irreproducible. In contrast, electroacupuncture (EA) has special characteristics in terms of objectivity and stability, thereby gaining considerable attention. Parameter setting plays a crucial role in EA practice. The current paper summarizes the current situation and limitations of parameter setting in EA practice...
July 20, 2020: Chinese Journal of Integrative Medicine
Emily G Facchine, Richard J Spontak, Orlando J Rojas, Saad A Khan
Cellulosic nanomaterials constitute a topic of growing commercial interest for numerous applications, many of which demand a working knowledge of the rheology of the materials. We demonstrate here that aqueous suspensions of micro/nanofibrillated cellulose (MNFC) exhibit complex shear behavior governed primarily by fibrillar floc dynamics. Regimes corresponding to structure formation, persistence and breakdown are quantitatively differentiated. We assess the recovery of the network structure as a function of the applied breakdown conditions and identify critical conditions that characterize the floc dynamics as isotropic or anisotropic...
July 20, 2020: Biomacromolecules
Chang-Hyuk Yoo, Jae-Kyoung Yu, Yeju Seong, Jun-Kyu Choi
Protein microarrays are miniaturized two-dimensional arrays, incorporating thousands of immobilized proteins, typically printed in minute amounts on functionalized solid substrates, which can be analyzed in a high-throughput fashion. Irreproducibility of the printing techniques adopted, resulting in inconsistently and nonuniformly deposited microscopic spots, nonuniform signal intensities from the printed microspots, and significantly high background noise are some of the critical issues that affect protein analysis using traditional protein microarrays...
July 14, 2020: ACS Omega
Thyago Leal-Calvo, Milton Ozório Moraes
Due to multiple hypothesis testing with often limited sample size, microarrays and other-omics technologies can sometimes produce irreproducible findings. Complementary to better experimental design, reanalysis and integration of gene expression datasets may help overcome reproducibility issues by identifying consistent differentially expressed genes from independent studies. In this work, after a systematic search, nine microarray datasets evaluating host gene expression in leprosy were reanalyzed and the information was integrated to strengthen evidence of differential expression for several genes...
July 13, 2020: Molecular Genetics and Genomics: MGG
Ann Borda, Kathleen Gray, Yuqing Fu
Background: Public engagement in health and biomedical research is being influenced by the paradigm of citizen science. However, conventional health and biomedical research relies on sophisticated research data management tools and methods. Considering these, what contribution can citizen science make in this field of research? How can it follow research protocols and produce reliable results? Objective: The aim of this article is to analyze research data management practices in existing biomedical citizen science studies, so as to provide insights for members of the public and of the research community considering this approach to research...
April 2020: JAMIA Open
John R Erb-Downward, Nicole R Falkowski, Jennifer C D'Souza, Lisa M McCloskey, Roderick A McDonald, Christopher A Brown, Kerby Shedden, Robert P Dickson, Christine M Freeman, Kathleen A Stringer, Betsy Foxman, Gary B Huffnagle, Jeffrey L Curtis, Sara D Adar
The bacterial microbiome of human body sites, previously considered sterile, remains highly controversial because it can be challenging to isolate signal from noise when low-biomass samples are being analyzed. We tested the hypothesis that stochastic sequencing noise, separable from reagent contamination, is generated during sequencing on the Illumina MiSeq platform when DNA input is below a critical threshold. We first purified DNA from serial dilutions of Pseudomonas aeruginosa and from negative controls using three DNA purification kits, quantified input using droplet digital PCR, and then sequenced the 16S rRNA gene in four technical replicates...
June 9, 2020: MBio
Radosław Gocoł, Marcin Malinowski, Jarosław Bis, Damian Hudziak, Joanna Ciosek, Wojciech Wojakowski, Ewa Gaszewska-Żurek, Marek Jasiński, Marek A Deja
BACKGROUND: Over the last years aortic valve repair has evolved from being a random and irreproducible procedure to a standardized technique yielding durable long-term results. AIMS: Long-term outcome of aortic valve repair and aortic valve sparing procedures. METHODS: We analyzed the outcomes of all consecutive patients who underwent aortic valve repair and/or aortic valve sparing root replacement till the end of 2019. We assessed mortality, freedom from reoperation and freedom from at least moderate aortic valve regurgitation...
June 2, 2020: Kardiologia Polska
Tian-Xing Zhang, Zhan-Zhan Zhang, Yu-Xin Yue, Xin-Yue Hu, Fan Huang, Linqi Shi, Yang Liu, Dong-Sheng Guo
Enhanced drug delivery can improve the therapeutic efficacy of drugs and help overcome side effects. However, many reported drug-delivery systems are too complex and irreproducible for practical use. In this work, the design of a hypoxia-responsive molecular container based on calixarene, called CAC4A, which presents a significant advance in practical, hypoxia-targeted drug-delivery, is reported. CAC4A enables a wide variety of clinical drugs to be quantitatively loaded to improve their solubility and stability, as well as enable the administration of reduced doses...
May 27, 2020: Advanced Materials
Hanfei Shen, Tony Liu, Jesse Cui, Piyush Borole, Ari Benjamin, Konrad Kording, David Issadore
Liquid biopsy (LB) technologies continue to improve in sensitivity, specificity, and multiplexing and can measure an ever growing library of disease biomarkers. However, clinical interpretation of the increasingly large sets of data these technologies generate remains a challenge. Machine learning is a popular approach to discover and detect signatures of disease. However, limited machine learning expertise in the LB field has kept the discipline from fully leveraging these tools and risks improper analyses and irreproducible results...
May 18, 2020: Lab on a Chip
Jinyang Zhang, Chao Su, Fergus J M Rogers, Nadim Darwish, Michelle L Coote, Simone Ciampi
Here, we investigate the development and relaxation of static charges on the surface of plastic materials that are first brought in contact, and then macroscopically separated. Experimentalists dealing with the static electrification of insulators are aware of difficulties predictably attaining, and precisely reproducing, a given charging magnitude. Here we have observed for the first time that in homo-systems (e.g. PTFE rubbed against PTFE) charge densities reach the maximum value after a material-specific contact time...
May 14, 2020: Physical Chemistry Chemical Physics: PCCP
Jenna E Gallegos, Neil R Adames, Mark F Rogers, Pavel Kraikivski, Aubrey Ibele, Kevin Nurzynski-Loth, Eric Kudlow, T M Murali, John J Tyson, Jean Peccoud
Over the last 30 years, computational biologists have developed increasingly realistic mathematical models of the regulatory networks controlling the division of eukaryotic cells. These models capture data resulting from two complementary experimental approaches: low-throughput experiments aimed at extensively characterizing the functions of small numbers of genes, and large-scale genetic interaction screens that provide a systems-level perspective on the cell division process. The former is insufficient to capture the interconnectivity of the genetic control network, while the latter is fraught with irreproducibility issues...
May 6, 2020: NPJ Systems Biology and Applications
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