Kaori Yasuda, Miyu Nishikawa, Kairi Okamoto, Kyohei Horibe, Hiroki Mano, Mana Yamaguchi, Risa Okon, Kimie Nakagawa, Naoko Tsugawa, Toshio Okano, Fumihiro Kawagoe, Atsushi Kittaka, Shinichi Ikushiro, Toshiyuki Sakaki
CYP24A1-deficient (Cyp24a1 KO) rats were generated using the CRISPER/Cas9 system to investigate CYP24A1-dependent or -independent metabolism of 25(OH)D3, the prohormone of calcitriol. Plasma 25(OH)D3 concentrations in Cyp24a1 KO rats were approximately twofold higher than in wild-type rats. Wild-type rats showed five metabolites of 25(OH)D3 in plasma following oral administration of 25(OH)D3, and these metabolites were not detected in Cyp24a1 KO rats. Among these metabolites, 25(OH)D3-26,23-lactone was identified as the second major metabolite with a significantly higher Tmax value than others...
January 2021: Journal of Biological Chemistry