Oscar Padilla, Hanna E Minnsm, Hong-Jian Wei, Weijia Fan, Andrea Webster-Carrionm, Masih Tazhibi, Nicholas M McQuillan, Xu Zhang, Matthew Gallitto, Rebecca Yeh, Zhiguo Zhang, Tom K Hei, Luca Szalontay, Jovana Pavisic, Yuewen Tan, Naresh Deoli, Guy Garty, James H Garvin, Peter D Canoll, Claire Vanpouille-Box, Vilas Menon, Marta Olah, Raul Rabadan, Cheng-Chia Wu, Robyn D Gartrell
PURPOSE: Diffuse Midline Glioma (DMG) is a fatal tumor traditionally treated with radiotherapy (RT) and previously characterized as having a non-inflammatory tumor immune microenvironment (TIME). FLASH is a novel RT technique using ultra-high dose rate, which is associated with decreased toxicity and effective tumor control. However, the effect of FLASH and conventional (CONV) RT on the DMG TIME have not yet been explored. METHODS: Here, we perform single-cell RNA sequencing and flow cytometry on immune cells isolated from an orthotopic syngeneic murine model of brainstem DMG following the use of FLASH (90Gy/sec) or CONV (2Gy/min) dose-rate RT, and compare to unirradiated tumor (SHAM)...
February 14, 2024: International Journal of Radiation Oncology, Biology, Physics