Jie Yang, Kui Ma, Cuiping Zhang, Yufan Liu, Feng Liang, Wenzhi Hu, Xiaowei Bian, Siming Yang, Xiaobing Fu
Neurological syndromes are observed in numerous patients who suffer burns, which add to the economic burden of societies and families. Recent studies have implied that blood-brain barrier (BBB) dysfunction is the key factor that induces these central nervous system (CNS) syndromes in peripheral traumatic disease, e.g., surgery and burns. However, the effect of burns on BBB and the underlying mechanism remains, largely, to be determined. The present study aimed to investigate the effect of burns on BBB and the potential of umbilical cord-derived mesenchymal stem cells (UC-MSCs), which have strong anti-inflammatory and repairing ability, to protect the integrity of BBB...
2020: Frontiers in Immunology
Chunjie Xu, Jing Zhao, Qiuyue Li, Lin Hou, Yan Wang, Siling Li, Fuyang Jiang, Zhonghui Zhu, Lin Tian
BACKGROUND: Silicosis is an occupational respiratory disease caused by long-term excessive silica inhalation, which is most commonly encountered in industrial settings. Unfortunately, there is no effective therapy to delay and cure the progress of silicosis. In the recent years, stem cell therapy has emerged as an attractive tool against pulmonary fibrosis (PF) owing to its unique biological characteristics. However, the direct use of stem cells remains limitation by many risk factors for therapeutic purposes...
November 25, 2020: Stem Cell Research & Therapy
Juehong Li, Zhixiao Yao, Hao Xiong, Haomin Cui, Xu Wang, Wei Zheng, Yun Qian, Cunyi Fan
BACKGROUND: Peritendinous fibrosis represents a fibrotic healing process that usually occurs after tendon injury or surgery. This worldwide challenge hampers the functional rehabilitation and the mobility of extremities. However, effective treatment is still lacking at present. The aim of our study was to explore the effect of extracellular vesicles derived from hydroxycamptothecin primed human umbilical cord stem cells (HCPT-EVs) on post-traumatic tendon adhesion. METHODS: Extracellular vesicles derived from unprimed human umbilical cord mesenchymal stem cells (Unprimed EVs) or HCPT-EVs were isolated and characterized...
November 25, 2020: Stem Cell Research & Therapy
Bahareh Abbaspanah, Samira Reyhani, Seyed Hadi Mousavi
Mesenchymal stem cells (MSCs) enable a novel approach in stem cell therapy. Bone marrow (BM) was the first source used in MSCs therapy. However, BM has a number of key limitations as a source of MSCs, such as existence of only a small number of MSCs in the tissue, the painful, ethically problematic, and invasive nature of the associated collection process, and decrease in MSC specifications as age of donors increases. As a result, there has been increasing scholarly attention in identifying alternative sources for MSCs...
November 24, 2020: Current Stem Cell Research & Therapy
Y Hao, Q X Yang, Q Wang, G C Xu, F Qi, C L Deng, Z R Wei, D L Wang
Objective: To investigate the feasibility of in vitro inflammatory wound microenvironment simulated by using inflammatory wound tissue homogenate of mice. Methods: (1) Ten eight-week-old C57BL/6 male mice were collected and full-thickness skin tissue with diameter of 1.0 cm on both sides of the midline of the back was taken with a perforator to make the normal skin tissue homogenate supernatant. At 48 h after the full-thickness skin defect wound was established, the wound tissue within 2 mm from the wound edge was taken to make inflammatory wound tissue homogenate supernatant...
November 20, 2020: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
Christophe Desterke, Frank Griscelli, Jusuf Imeri, Paul Marcoux, Thomas Lemonnier, Theodoros Latsis, Ali G Turhan, Annelise Bennaceur-Griscelli
The use of mesenchymal stem cells (MSC) derived from several sources as a major anti-inflammation strategy has been suggested in the recent outbreak of Coronavirus-19 (COVID-19). As the virus enters the target cells through the receptor ACE2, it is important to determine if the MSC population transfused to patients could also be a target for the virus entry. We report here that ACE2 is highly expressed in adult bone marrow, adipose tissue or umbilical cord-derived MSC. On the other hand, placenta-derived MSC express low levels of ACE2 but only in early passages of cultures...
November 25, 2020: Stem Cells Translational Medicine
Teresa Puig-Pijuan, Mariana A de Godoy, Luiza Rachel Pinheiro Carvalho, Victor Bodart-Santos, Rafael Soares Lindoso, Pedro Moreno Pimentel-Coelho, Rosalia Mendez-Otero
Aim: Mesenchymal stem cells (MSCs) have neuroprotective and immunomodulatory properties, which are partly mediated by extracellular vesicles (EVs) secretion. We aimed to evaluate the effects of human Wharton's jelly-derived MSCs (WJ-MSCs) and their EVs on rat hippocampal cultures subjected to hydrogen peroxide (H2 O2 ). Materials & methods: Hippocampal dissociated cultures were either co-cultured with WJ-MSCs or treated with their EVs prior to H2 O2 exposure and reactive oxygen species levels and cell viability were evaluated...
September 17, 2020: Future Science OA
Wenwen Cai, Jinghua Sun, Yao Sun, Xuhui Zhao, Chunyan Guo, Jie Dong, Xiaoyang Peng, Ruiping Zhang
Acetaminophen (APAP) has been widely used for relieving pain and fever, whilst overdose would lead to the occurrence of acute liver failure (ALF). Currently, few effective treatments are available for ALF in clinic, especially for severe, advanced- or end-stage patients who need liver transplantation. Human umbilical cord-derived mesenchymal stem cells (hUMSCs), as one of the mesenchymal stem cells, not only contribute to relieving hepatotoxicity and promoting hepatocyte regeneration due to their self-renewing, multi-differentiation potential, anti-inflammatory, immunomodulatory and paracrine properties, but possess lower immunomodulatory effects, faster self-renewal properties and noncontroversial ethical concerns, which may play a better role in the treatment of ALF...
December 7, 2020: Biomaterials Science
Parisa Kangari, Tahereh Talaei-Khozani, Iman Razeghian-Jahromi, Mahboobeh Razmkhah
Skeletal disorders are among the leading debilitating factors affecting millions of people worldwide. The use of stem cells for tissue repair has raised many promises in various medical fields, including skeletal disorders. Mesenchymal stem cells (MSCs) are multipotent stromal cells with mesodermal and neural crest origin. These cells are one of the most attractive candidates in regenerative medicine, and their use could be helpful in repairing and regeneration of skeletal disorders through several mechanisms including homing, angiogenesis, differentiation, and response to inflammatory condition...
November 23, 2020: Stem Cell Research & Therapy
Hui Li, Qi Liu, Xiaofeng Gao, Di Zhang, Shengjun Mao, Yongqian Jia
Graft-versus-host disease (GVHD) is a frequent complication following allogeneic hematopoietic stem cell transplantation (allo-HSCT). The application of mesenchymal stromal cells (MSCs) to treat GVHD patients refractory to initial steroid treatment has led to impressive results. In this study, we explored the potential of human umbilical mesenchymal stem cells (HUMSCs) transfected with the IFN-γ gene of human (h)/ mice (m) (HUMSCs+Ad-h/mIFN-γ) carried by a recombinant adenoviral vector in the prevention and treatment of GVHD...
November 18, 2020: International Journal of Pharmaceutics
Yang Yang, Mao Pang, Cong Du, Zhong-Yu Liu, Zi-Hao Chen, Nan-Xiang Wang, Liang-Ming Zhang, Yu-Yong Chen, Jian Mo, Jian-Wen Dong, Pei-Gen Xie, Qi-You Wang, Bin Liu, Li-Min Rong
BACKGROUND AIMS: Stem cell transplantation is a potential treatment for intractable spinal cord injury (SCI), and allogeneic human umbilical cord mesenchymal stem cells (hUC-MSCs) are a promising candidate because of the advantages of immune privilege, paracrine effect, immunomodulatory function, convenient collection procedure and little ethical concern, and there is an urgent need to develop a safe and effective protocol regarding their clinical application. METHODS: A prospective, single-center, single-arm study in which subjects received four subarachnoid transplantations of hUC-MSCs (1 × 106 cells/kg) monthly and were seen in follow-up four times (1, 3, 6 and 12 months after final administration) was conducted...
November 17, 2020: Cytotherapy
Hind Rafei, May Daher, Katayoun Rezvani
Chimeric antigen receptor (CAR) T cells are a rapidly emerging form of cancer treatment, and have resulted in remarkable responses in refractory lymphoid malignancies. However, their widespread clinical use is limited by toxicity related to cytokine release syndrome and neurotoxicity, the logistic complexity of their manufacturing, cost and time-to-treatment for autologous CAR-T cells, and the risk of graft-versus-host disease (GvHD) associated with allogeneic CAR-T cells. Natural killer (NK) cells have emerged as a promising source of cells for CAR-based therapies due to their ready availability and safety profile...
November 20, 2020: British Journal of Haematology
Maura Mingoia, Cristian A Caria, Lin Ye, Isadora Asunis, M Franca Marongiu, Laura Manunza, M Carla Sollaino, Jiaming Wang, Annalisa Cabriolu, Ryo Kurita, Yukio Nakamura, Francesco Cucca, Yuet W Kan, M Giuseppina Marini, Paolo Moi
Hereditary persistence of fetal haemoglobin (HPFH) is the major modifier of the clinical severity of β-thalassaemia. The homozygous mutation c.-196 C>T in the Aγ-globin (HBG1) promoter, which causes Sardinian δβ0 -thalassaemia, is able to completely rescue the β-major thalassaemia phenotype caused by the β0 39-thalassaemia mutation, ensuring high levels of fetal haemoglobin synthesis during adulthood. Here, we describe a CRISPR/Cas9 genome-editing approach, combined with the non-homologous end joining (NHEJ) pathway repair, aimed at reproducing the effects of this naturally occurring HPFH mutation in both HBG promoters...
November 20, 2020: British Journal of Haematology
Maryanne C Herzig, Christopher P Delavan, Katherine J Jensen, Carolina Cantu, Robbie K Montgomery, Barbara A Christy, Andrew P Cap, James A Bynum
BACKGROUND: Mesenchymal stromal cells (MSCs) have been proposed for treatment of acute respiratory distress syndrome (ARDS), graft versus host disease (GVHD), wound healing and trauma. A consensus is building that immunomodulation by MSCs is important for therapeutic potential. MSCs suppress peripheral blood mononuclear cell (PBMC) proliferation in vitro, potentially reflecting an ability to suppress PBMC inflammatory responses in vivo. Current mixed lymphocyte reaction (MLR) assays commonly used to evaluate MSC potency generally rely on either direct co-culture or indirect culture using transwell systems for monitoring the proliferation of isolated PBMCs in the presence of mitotically inactive MSCs...
November 16, 2020: Journal of Immunological Methods
Han Qiu, Tianyang Qian, Tong Wu, Xiaoyang Wang, Changlian Zhu, Chao Chen, Laishuan Wang
Preterm birth is a global public health problem. A large number of preterm infants survive with preterm white matter injury (PWMI), which leads to neurological deficits, and has multifaceted etiology, clinical course, monitoring, and outcomes. The principal upstream insults leading to PWMI initiation are hypoxia-ischemia and infection and/or inflammation and the key target cells are late oligodendrocyte precursor cells. Current PWMI treatments are mainly supportive, and thus have little effect in terms of protecting the immature brain or repairing injury to improve long-term outcomes...
November 18, 2020: Journal of Neuroscience Research
Yunxiuxiu Xu, Linhui Cao, Guangcheng Chen, Liang Chen, Yanshan Li, Yu Lai, Hanqin Weng, Tao Chen, Linyun Wang, Yibiao Ye
Objective : Exosomes derived from human umbilical cord mesenchymal stem cells (hucMSCs) expressing microRNAs (miRNAs) have been highlighted in human cancers. However, the detailed molecular mechanism of hucMSCs-derived exosomal miR-451a on hepatocellular carcinoma (HCC) remains further investigation. Our study aims to explore the impact of exosomal miR-451a on the progression of HCC. Methods : Expression of miR-451a and a disintegrin and metalloprotease 10 (ADAM10) in HCC tissues and adjacent normal tissues was determined...
November 18, 2020: RNA Biology
Xiao Han, Haoqing Yang, Huina Liu, Chen Zhang, Yangyang Cao, Zhipeng Fan, Ruitang Shi
Human umbilical cord mesenchymal stem cells (HUCMSCs) can be obtained from different parts of the umbilical cord, including Wharton's jelly. Transplantation of Wharton's jelly umbilical cord stem cells (WJCMSCs) is a promising strategy for the treatment of various diseases. However, the molecular mechanisms underlying the proliferation of WJCMSCs are incompletely understood. Here, we report that overexpression of miR-196b-5p in WJCMSCs suppresses proliferation and arrests the cell cycle in G0/G1 phase, whereas knock-down of miR-196b-5p promotes WJCMSC proliferation and cell cycle progression...
November 18, 2020: FEBS Open Bio
Sowmithra Sowmithra, Nishtha Kusum Jain, Ramesh Bhonde, Indrani Datta
Increasing evidence suggests that mesenchymal stem cells(MSCs) have beneficial effects in hypoxic ischemic reperfusion injury, but the underlying mechanisms are unclear. Here, we first examined the effect of OGD reperfusion injury on the vulnerability of human NPs derived from human embryonic stem cells (hESCs) with regard to cell survival and oxidative stress. Cellular deregulation was assessed by measuring glutathione levels, basal calcium and intracellular calcium [Ca2+ ]i response under KCl stimulation, as well as the key parameters of proliferation, glial progenitor marker expression and migration...
November 18, 2020: Cellular and Molecular Neurobiology
Ying Feng, Jianying Huang, Jianyuan Wu, Yan Xu, Bo Chen, Lijun Jiang, Hui Xiang, Zhiyong Peng, Xinghuan Wang
OBJECTIVES: We aim to explore the safety and feasibility of umbilical cord mesenchymal stem cells (UC-MSCs) transplantation in patients with severe and critically severe coronavirus disease-2019 (COVID-19). METHODS: We conducted a small sample, single arm, pilot trial. In addition to standard therapy, we performed four rounds of transplantation of UC-MSCs in sixteen patients with severe and critically severe COVID-19. We recorded adverse events from enrolment to Day 28...
November 17, 2020: Cell Proliferation
Ekaterina Silina, Natalia Manturova, Victor Stupin
Purpose: An assessment of the effectiveness of progenitor mesenchymal stem cell as injections and as part of a polymer hydrogel for the wounds treatment. Materials and Methods: Fixed-size wounds (average area of 135.8 mm2 ) were modeled on the back of white Wistar rats, aged 9 months. Mesenchymal stem cells (MSC) isolated from a human umbilical cord were injected into the wounds once on the modeling day (SC group). In other animals, MSC were periodically applied externally as one of the components in the polymer hydrogel (Polymer_sc group)...
2020: Stem Cells and Cloning: Advances and Applications
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