Guilherme C L Reis, Bruno M Dala-Paula, Olga L Tavano, Leticia R Guidi, Helena T Godoy, Maria Beatriz A Gloria
Mushrooms are significant sources of amino acids and bioactive amines; however, their bioaccessibility can be affected by processing and during in vitro digestion. Fresh Agaricus bisporus mushroom was submitted to cooking and canning and samples were submitted to in vitro gastric and gastric-intestinal digestions. An UHPLC method was used for the simultaneous determination of 18 free amino acids, 10 biogenic amines and ammonia in the samples. Fresh mushroom contained 14 free amino acids, with predominance of alanine and glutamic acid; spermidine was the only amine detected; and ammonia was also detected...
November 2020: Food Research International
Yu Meng Wang, Wei Yi Wang, Li Yan Sun, Qiang Li, Zhi Jun Zhao, Jian Hu, Rong Yu Zhang, Li Hua Wang
Objective: To explore potential serum biomarkers of children with Kashin-Beck Disease (KBD) and the metabolic pathways to which the biomarkers belong. Methods: A two-stage metabolomic study was employed. The discovery cohort included 56 patients, 51 internal controls, and 50 external controls. The metabolites were determined by HPLC-(Q-TOF)-MS and confirmed by Human Metabolome Databases (HMDB) and Metlin databases. MetaboAnalyst 3.0 and the Kyoto Encyclopedia of Genes and Genomes (KEGG) database were used to analyze the metabolic pathways of the candidate metabolites...
October 20, 2020: Biomedical and Environmental Sciences: BES
Thomas Pekar, Katharina Bruckner, Susanne Pauschenwein-Frantsich, Anna Gschaider, Martina Oppliger, Julia Willesberger, Petra Ungersbäck, Aribert Wendzel, Alexandra Kremer, Walter Flak, Felix Wantke, Reinhart Jarisch
The worldwide prevalence of dementia is estimated at 35.6 million and will rise to 115 million by 2050. There is therefore an urgent need for well-founded dementia diagnostics and well-researched therapeutic options. Previous studies have highlighted that spermidine has the ability to trigger the important process of dissolving amyloid-beta plaques by autophagy. They also confirmed that nutritional intervention with the natural polyamine spermidine can prevent memory loss in aging model organisms. This multicentric double-blind preliminary study focused on the effect of oral spermidine supplementation on older adults' cognitive performance...
November 19, 2020: Wiener Klinische Wochenschrift
Manoj Manickam, Pulla Reddy Boggu, Thanigaimalai Pillaiyar, Yeo Jin Nam, Md Abdullah, Seung Jin Lee, Jong Seong Kang, Sang-Hun Jung
In continuation with the previous work, a series of 5-hydroxy-2-amidomethoxy-1,4-naphthoquinones were prepared to establish the structure-activity relationship studies toward anticancer activity (IC50 in µM) against three cell lines; colo205 (colon adenocarcinoma), T47D (breast ductal carcinoma) and K562 (chronic myelogenous leukemia). Among the synthesized compounds, naphthoquinone amines, 5 (0.8; 0.6; 0.8), 14 (0.8; 0.6; 0.5) and the amine precursor, 4 (1.3; 0.3; 1.0) displayed potent anticancer activities...
November 13, 2020: Bioorganic & Medicinal Chemistry Letters
Pauline Dontaine, Elisa Kottos, Martine Dassonville, Ovidiu Balasel, Véronique Catros, Julie Soblet, Pascale Perlot, Catheline Vilain
Snyder-Robinson syndrome (OMIM #309583) is a rare X-linked condition, caused by mutation in the SMS gene (MIM *300105), characterized by a wide spectrum of clinical signs including developmental delay, epilepsy, asthenic habitus, dysmorphism, osteopenia, and renal or genital anomalies. Here we describe two maternal half-brothers who both presented with severe neurodevelopmental delay, seizures, hearing loss, facial dysmorphism, renal and ophthalmologic anomalies, failure to thrive and premature death. A novel p...
November 10, 2020: European Journal of Medical Genetics
Lingyan Ma, Yinhua Ni, Luting Hu, Yufeng Zhao, Liujie Zheng, Song Yang, Liyang Ni, Zhengwei Fu
AIMS: The therapeutic effects of spermidine on preexisting obese mice have been not fully elucidated. In this study, we assessed the anti-obesity impact of spermidine on high-fat diet (HFD)-induced obese mice. MAIN METHODS: C57BL/6J mice were fed a HFD for 16 weeks to induce obesity, and then treated with or without spermidine via drinking water for additional 8 weeks. The contributions of spermidine in regulating obesity phenotypes and metabolic syndrome were further evaluated...
November 10, 2020: Life Sciences
T T Dunston, M A Khomutov, S B Gabelli, T M Stewart, J R Foley, S N Kochetkov, A R Khomutov, R A Casero
Homeostasis of the biogenic polyamines spermine (Spm) and spermidine (Spd), present in μM-mM concentrations in all eukaryotic cells, is precisely regulated by coordinated activities of the enzymes of polyamine synthesis, degradation, and transport, in order to sustain normal cell growth and viability. Spermine oxidase (SMOX) is the key and most recently discovered enzyme of polyamine metabolism that plays an essential role in regulating polyamine homeostasis by catalyzing the back-conversion of Spm to Spd...
July 2020: Acta Naturae
Yuemei Zhang, Yu Wang, Wenxu Wen, Zhengrong Shi, Qinsheng Gu, Golam Jalal Ahammed, Kai Cao, Mohammad Shah Jahan, Sheng Shu, Jian Wang, Jin Sun, Shirong Guo
Autophagy, an evolutionally conserved cellular degradation process, plays critical roles in plant development and stress response. Despite the wealth of information on the vital role of autophagy in responses to environmental stresses, little is known about the regulation of autophagy. In this study, we demonstrated that spermidine (Spd), a kind of polyamine, was involved in the regulation of salt tolerance through activating the expression of ATG (autophagy-related) genes and the formation of autophagosomes in cucumber under salt stress...
November 10, 2020: Autophagy
Maya Matsunami, Kyoko Toyofuku, Natsumi Kimura, Atsushi Ogawa
To breed osmotic stress-tolerant rice, the mechanisms involved in maintaining root growth under osmotic stress is important to elucidate. In this study, two rice ( Oryza sativa L.) cultivars, IR 58 (stress-tolerant cultivar) and Basilanon (stress-sensitive cultivar), were used. After 1, 3, and 7 days of -0.42 MPa osmotic stress treatment induced by polyethylene glycol (PEG) 6000, root metabolomes were analyzed, yielding 276 detected compounds. Among 276 metabolites, 102 metabolites increased with the duration of the stress treatment in IR 58 roots, and only nine metabolites decreased...
November 6, 2020: Plants (Basel, Switzerland)
Nadia Botini, Felipe Astolpho Almeida, Kaliane Zaira Camacho Maximiano da Cruz, Ricardo Souza Reis, Ellen Moura Vale, Ana Beatriz Garcia, Claudete Santa-Catarina, Vanildo Silveira
Somatic embryogenesis is an important biotechnological technique for large-scale propagation of elite genotypes. Identifying stage-specific compounds associated with somatic embryo development can help elucidate the ontogenesis of Carica papaya L. somatic embryos and improve tissue culture protocols. To identify the stage-specific proteins that are present during the differentiation of C. papaya somatic embryos, proteomic analyses of embryos at the globular, heart, torpedo and cotyledonary developmental stages were performed...
November 5, 2020: Biochimica et Biophysica Acta. Proteins and Proteomics
Lingyan Ma, Yinhua Ni, Zhe Wang, Wenqing Tu, Liyang Ni, Fen Zhuge, Aqian Zheng, Luting Hu, Yufeng Zhao, Liujie Zheng, Zhengwei Fu
Obesity is associated with impaired intestinal barrier function and dysbiosis of the gut microbiota. Spermidine, a polyamine that acts as an autophagy inducer, has important benefits in patients with aging-associated diseases and metabolic dysfunction. However, the mechanism of spermidine on obesity remains unclear. Here, we show that spermidine intake is negatively correlated with obesity in both humans and mice. Spermidine supplementation causes a significant loss of weight and improves insulin resistance in diet-induced obese (DIO) mice...
November 9, 2020: Gut Microbes
Stephanie Lilja, Julia Oldenburg, Angelika Pointner, Laura Dewald, Mariam Lerch, Berit Hippe, Olivier Switzeny, Alexander Haslberger
Aim: We investigated different bioactive compounds including epigallocatechin gallate (EGCG), anthocyanidin, resveratrol, phloretin, spermidine, butyrate, and β -hydroxybutyrate with regard to their effect on SIRT3 via NRF2 and modulation of the proinflammatory senescence-associated secretory phenotype (SASP) in senescence induced 3T3-L1 preadipocytes. Methods: For induction of senescence, 3T3-L1 preadipocytes were incubated with bromodeoxyuridine (BrdU) for 8 days...
2020: Oxidative Medicine and Cellular Longevity
Huimin Zhao, Yaqi Jia, Yanting Cao, Yucheng Wang
Agrobacterium tumefaciens -mediated gene transfer is the most commonly used method for plant genetic engineering. However, during the period of A. tumefaciens culture, the effects of Agrobacterium culture before inoculation on genetic transformation are poorly understood. In the present study, we investigated the factors that affect the genetic transformation efficiency during Agrobacterium culture using Tamarix hispida as transgenic plant material. Agrobacterium treatment with spermidine (Spe), azacitidine (5-AzaC), dithiothreitol (DTT), or acetosyringone (AS) alone all significantly improved the efficiency of T-DNA transfer...
2020: Frontiers in Plant Science
Bradley S Gordon, Michael L Rossetti, Robert A Casero
Maintaining a critical amount of skeletal muscle mass is linked to reduced morbidity and mortality. In males, testicular androgens regulate muscle mass with a loss of androgens being critical as it is associated with muscle atrophy. Atrophy of the limb muscles is particularly important, but the pathways by which androgens regulate limb muscle mass remain equivocal. We used microarray analysis to identify changes to genes involved with polyamine metabolism in the tibialis anterior (TA) muscle of castrated mice...
October 30, 2020: Applied Physiology Nutrition and Metabolism
Alexandre Duprey, Eduardo A Groisman
DNA supercoiling is essential for all living cells because it controls all processes involving DNA. In bacteria, global DNA supercoiling results from the opposing activities of topoisomerase I, which relaxes DNA, and DNA gyrase, which compacts DNA. These enzymes are widely conserved, sharing >91% amino acid identity between the closely related species Escherichia coli and Salmonella enterica serovar Typhimurium. Why, then, do E. coli and Salmonella exhibit different DNA supercoiling when experiencing the same conditions? We now report that this surprising difference reflects disparate activation of their DNA gyrases by the polyamine spermidine and its precursor putrescine...
October 2020: PLoS Genetics
Qian Feng, Yuanmeng Li, Yuwei Yang, Jiafu Feng
Objective: Diabetic kidney disease (DKD) leads to low high albuminuria and gradually progresses to very high albuminuria with kidney insufficiency. However, about 20-40% of DKD is normoalbuminuric DKD (NADKD), which has impaired kidney function but normal urine albumin. This study is to investigate the urine metabolomic profiles of patients with NADKD and albuminuria DKD (ADKD). Methods: In total, 95 patients were divided into a simple diabetes mellitus group (SDM group), an ADKD group, and a NADKD group...
2020: Frontiers in Physiology
Rui Liu, Xiaolei Li, Hui Ma, Qian Yang, Qianwen Shang, Lin Song, Zhiyuan Zheng, Shengchao Zhang, Yongsha Pan, Peiqing Huang, Jiankai Fang, Yanan Li, Zhanhong Liu, Lijuan Cao, Chao Feng, Zheng Gong, Yongjing Chen, Ying Wang, Gerry Melino, Changshun Shao, Yufang Shi
Distinct metabolic programs, either energy-consuming anabolism or energy-generating catabolism, were required for different biological functions. Macrophages can adopt different immune phenotypes in response to various cues and exhibit anti- or pro-inflammatory properties relying on catabolic pathways associated with oxidative phosphorylation (OXPHOS) or glycolysis. Spermidine, a natural polyamine, has been reported to regulate inflammation through inducing anti-inflammatory (M2) macrophages. However, the underlying mechanisms remain elusive...
October 24, 2020: Free Radical Biology & Medicine
Zhou Li, Bizhen Cheng, Yan Peng, Yan Zhang
The frequency and severity of global abiotic stresses such as heat, drought, and salt stress are increasing due to climate changes. Objectives of this study were to investigate effects of γ-aminobutyric acid (GABA) priming on inducing plants' acclimation to abiotic stress associated with alterations of endogenous polyamines (PAs), amino acids, and sugars in creeping bentgrass (Agrostis stolonifera). The pretreatment with GABA fertigation significantly alleviated heat-, drought-, and salt-induced declines in leaf relative water content, chlorophyll content, cell membrane stability, photochemical efficiency (Fv/Fm), and performance index on absorption basis (PIABS), and also further decreased stress-caused decline in osmotic potential in leaves...
October 23, 2020: Plant Physiology and Biochemistry: PPB
Shuai Zhang, Hang Gao, Xiongkun Yuan, Junjun Wang, Jianjun Zang
This study explores the energy partition patterns of modern growing pigs at 25 kg and 65 kg raised at gradient-ambient temperatures. It also investigates the underlying changes in plasma under such conditions, based on the integrative analysis of indirect calorimetry and non-target metabolomics profiling. Thirty-six barrows with initial BW of 26.4 ± 1.9 kg and 24 barrows with initial BW of 64.2 ± 3.1 kg were successively allotted to six respiration chambers with ambient temperatures set as 18 °C, 21 °C, 23 °C, 27 °C, 30 °C, and 32 °C, and four respiration chambers with ambient temperatures set as 18 °C, 23 °C, 27 °C, and 32 °C, respectively...
October 23, 2020: Animals: An Open Access Journal From MDPI
Ying Xiao, Chunling Li, Hongyue Wang, Yijun Liu
We explored the mechanism by which LINC00265 regulates angiogenesis of osteosarcoma cells via the miR-382-5p/spermidine/spermine N1 -acetyltransferase-1 (SAT1) and miR-382-5p/vav guanine nucleotide exchange factor 3 (VAV3) axis. Cell scratch assay, Transwell assay and tube formation assay were applied to detect cell migration, invasion and tube formation abilities. The effects of LINC00265 targeting miR-382-5p in osteosarcoma in vivo were studied using a tumour-burden assay. A total of 70 genes potentially involved in osteosarcoma angiogenesis were identified, and a Gene Ontology (GO) analysis found that SAT1 and VAV3 were closely related to angiogenesis...
August 14, 2020: Aging
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