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Kaisa Vuornos, Miina Ojansivu, Janne T Koivisto, Heikki Häkkänen, Birhanu Belay, Toni Montonen, Heini Huhtala, Minna Kääriäinen, Leena Hupa, Minna Kellomäki, Jari Hyttinen, Janne A Ihalainen, Susanna Miettinen
BACKGROUND: Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2 O 12.1, K2 O 14.0, CaO 19.8, P2 O5 2.5, B2 O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development. GG hydrogels were crosslinked with spermidine (GG-SPD) or BaG extract (GG-BaG)...
June 2019: Materials Science & Engineering. C, Materials for Biological Applications
Ning Liu, Zhaolai Dai, Yunchang Zhang, Hai Jia, Jiangqing Chen, Shiqiang Sun, Guoyao Wu, Zhenlong Wu
We recently reported that dietary supplementation with L-proline (proline) during gestation improved embryonic survival in C57BL/6J mice. The objective of the present study was to test the hypothesis that the effect of maternal proline supplementation on embryonic survival can be carried forward to the first generation female offspring. In the F0 generation, pregnant dams were fed a purified diet supplemented with 0 (control) or 5 g proline/kg diet. The F1 female adult offsprings were bred to fertile males...
March 16, 2019: Amino Acids
Gabriela Guédez, Apiradee Pothipongsa, Saija Sirén, Arto Liljeblad, Saowarath Jantaro, Aran Incharoensakdi, Tiina A Salminen
Spermidine is a ubiquitious polyamine synthesised by spermidine synthase (SPDS) from the substrates putrescine and decarboxylated S-adenosylmethionine (dcAdoMet). SPDS generally is active as homodimer but higher oligomerization states have been reported in SPDS from thermophiles, which are less specific to putrescine as the amino acceptor substrate. Several crystal structures of SPDS have been solved with and without bound substrates and/or products, as well as inhibitors. Here, we determined the crystal structure of SPDS from the cyanobacterium Synechococcus ( Sy SPDS) that is a homodimer, which we also observed in solution...
March 15, 2019: Biochemical Journal
Pengfei Liu, Montserrat Rojo de la Vega, Matthew Dodson, Fei Yue, Boyun Shi, Deyu Fang, Eli Chapman, Leyuan Liu, Donna D Zhang
Spermidine, a naturally occurring polyamine, has been recognized as a caloric restriction mimetic that confers health benefits, presumably by inducing autophagy. Recent studies have reported that oral administration of spermidine protects against liver fibrosis and hepatocarcinogenesis through activation of MAP1S-mediated autophagy. NRF2 is a transcription factor that mediates cellular protection by maintaining the cell's redox, metabolic, and proteostatic balance. In this study, we demonstrate that spermidine is a non-canonical NRF2 inducer, and that MAP1S is a novel component of this non-canonical pathway of NRF2 activation...
March 15, 2019: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
Tengfei Zhao, Changjian Wang, Feng Bai, Siqi Li, Chunxian Yang, Fangyuan Zhang, Ge Bai, Min Chen, Xiaozhong Lan, Zhihua Liao
Ornithine decarboxylase (ODC) catalyzes ornithine decarboxylation to yield putrescine, a key precursor of polyamines, and tropane alkaloids (TAs). Here, to investigate in depth the role of ODC in polyamine/TA biosynthesis and to provide a candidate gene for engineering polyamine/TA production, the ODC gene ( HnODC ) was characterized from Hyoscyamus niger , a TA-producing plant. Our phylogenetic analysis revealed that HnODC was clustered with ODC enzymes of plants. Experimental work showed HnODC highly expressed in H...
2019: Frontiers in Plant Science
Taro Fukui, Kuniyasu Soda, Koichi Takao, Toshiki Rikiyama
We first demonstrated that long-term increased polyamine (spermine, spermidine, putrescine) intake elevated blood spermine levels in mice and humans, and lifelong consumption of polyamine-rich chow inhibited aging-associated increase in aberrant DNA methylation, inhibited aging-associated pathological changes, and extend lifespan of mouse. Because gene methylation status is closely associated with aging-associated conditions and polyamine metabolism is closely associated with regulation of gene methylation, we investigated the effects of extracellular spermine supplementation on substrate concentrations and enzyme activities involved in gene methylation...
March 12, 2019: International Journal of Molecular Sciences
Elena Sánchez-Elordi, Laura Morales de Los Ríos, Carlos Vicente, María-Estrella Legaz
Previous studies have already highlighted the correlation between Sporisorium scitamineum pathogenicity and sugarcane polyamine accumulation. It was shown that high infectivity correlates with an increase in the amount of spermidine, spermine and cadaverine conjugated to phenols in the sensitive cultivars whereas resistant plants mainly produce free putrescine. However, these previous studies did not clarify the role of these polyamides in the disorders caused to the plant. Therefore, the purpose of this research is to clarify the effect of polyamines on the development of smut disease...
March 12, 2019: Journal of Plant Research
Monika A Olszewska, Joanna Kolodziejczyk-Czepas, Magdalena Rutkowska, Anna Magiera, Piotr Michel, Marcin W Rejman, Pawel Nowak, Aleksandra Owczarek
Polyphenol-rich plant extracts might alleviate the negative impact of oxidative stress and inflammation, but careful phytochemical standardisation and evaluation of various mechanisms are required to fully understand their effects. In this context, flower extracts of Sorbus aucuparia L.-a traditional medicinal plant-were investigated in the present work. The LC-MS/MS profiling of the extracts, obtained by fractionated extraction, led to the identification of 66 constituents, mostly flavonols (quercetin and sexangularetin glycosides with dominating isoquercitrin), pseudodepsides of quinic and shikimic acids (prevailing isomers of chlorogenic acid and cynarin), and flavanols (catechins and proanthocyanidins)...
2019: Oxidative Medicine and Cellular Longevity
Xiaobei Pan, Emma L Cunningham, Anthony P Passmore, Bernadette McGuinness, Daniel F McAuley, David Beverland, Seamus O'Brien, Tim Mawhinney, Jonathan M Schott, Henrik Zetterberg, Brian D Green
Delirium is a marker of brain vulnerability, associated with increasing age, pre-existing cognitive impairment and, recently, cerebrospinal fluid (CSF) biomarkers of Alzheimer's disease. This nested case-control study used a targeted quantitative metabolomic methodology to profile the preoperative CSF of patients (n = 54) who developed delirium following arthroplasty (n = 28) and those who did not (n = 26). The aim was to identify novel preoperative markers of delirium, and to assess potential correlations with clinical data...
March 12, 2019: Scientific Reports
Seyed Morteza Zahedi, Marjan Sadat Hosseini, Mahdieh Karimi, Asghar Ebrahimzadeh
Mango is a tropical fruit which is sensitive to chilling injury. The present work investigated the potential of edible coatings of chitosan and polyamine spermidine in increasing shelf life and quality of mango. The control fruits (treated with distilled water) and the mango fruits treated with different concentrations of chitosan (0.5%, 1.0%, and 2.0%) and spermidine (0.5, 1.0, and 2.0 mM) were studied to improve postharvest characteristics and quality maintenance during cold storage. Parameters such as firmness, weight loss, fungal contamination, total phenol, antioxidant activity, vitamin C, pH, total soluble solids (TSS), titratable acidity (TA), flavor index, color index, and ethylene production were measured after at harvest (0), 8, 16, and 24 days of storage at 15 ± 2°C and relative humidity of 85%-90%...
February 2019: Food Science & Nutrition
Kamal El Bissati, Henry Redel, Li-Min Ting, Joseph D Lykins, Martin J McPhillie, Rajendra Upadhya, Patrick M Woster, Nigel Yarlett, Kami Kim, Louis M Weiss
Twenty-two compounds belonging to several classes of polyamine analogs have been examined for their ability to inhibit the growth of the human malaria parasite Plasmodium falciparum in vitro and in vivo . Four lead compounds from the thiourea sub-series and one compound from the urea-based analogs were found to be potent inhibitors of both chloroquine-resistant (Dd2) and chloroquine-sensitive (3D7) strains of Plasmodium with IC50 values ranging from 150 to 460 nM. In addition, the compound RHW, N 1, N 7-bis (3-(cyclohexylmethylamino) propyl) heptane-1,7-diamine tetrabromide was found to inhibit Dd2 with an IC50 of 200 nM...
2019: Frontiers in Cellular and Infection Microbiology
Sukhmeen Kaur Kohli, Shagun Bali, Ruchi Tejpal, Vandana Bhalla, Vinod Verma, Renu Bhardwaj, A A Alqarawi, Elsayed Fathi Abd Allah, Parvaiz Ahmad
Lead (Pb) toxicity is a major environmental concern affirming the need of proper mitigation strategies. In the present work, potential of combined treatment of 24-Epibrassinolide (24-EBL) and Salicylic acid (SA) against Pb toxicity to Brassica juncea L. seedlings were evaluated. Seedlings pre-imbibed in EBL (0.1 mM) and SA (1 mM) individually and in combination, were sown in Pb supplemented petri-plates (0.25, 0.50 and 0.75 mM). Various microscopic observations and biochemical analysis were made on 10 days old seedlings of B...
March 5, 2019: Scientific Reports
Amalia Forte, Carmela Rita Balistreri, Marisa De Feo, Alessandro Della Corte, Per Hellstrand, Lo Persson, Bengt-Olof Nilsson
Polyamines are small aliphatic cationic molecules synthesized via a highly regulated pathway and involved in general molecular and cellular phenomena. Both mammalian cells and microorganisms synthesize polyamines, and both sources may contribute to the presence of polyamines in the circulation. The dominant location for microorganisms within the body is the gut. Accordingly, the gut microbiota probably synthesizes most of the polyamines in the circulation in addition to those produced by the mammalian host cells...
February 27, 2019: Journal of Molecular and Cellular Cardiology
Rosa Maria Borzì, Silvia Cetrullo, Stefania D'Adamo, Manuela Minguzzi, Flavio Flamigni
No abstract text is available yet for this article.
March 1, 2019: Experimental & Molecular Medicine
Pradeep K Sacitharan, George Bou Gharios, James R Edwards
No abstract text is available yet for this article.
March 1, 2019: Experimental & Molecular Medicine
Barbara U Metzler-Zebeli, Sina-Catherine Siegerstetter, Elizabeth Magowan, Peadar G Lawlor, Niamh E O'Connell, Qendrim Zebeli
Restrictive feeding influences systemic metabolism of nutrients; however, this impact has not been evaluated in chickens of diverging feed efficiency. This study investigated the effect of ad libitum versus restrictive feeding (85% of ad libitum) on the serum metabolome and white blood cell composition in chickens of diverging residual feed intake (RFI; metric for feed efficiency). Blood samples were collected between days 33 and 37 post-hatch. While serum glucose was similar, serum uric acid and cholesterol were indicative of the nutritional status and chicken's RFI, respectively...
February 25, 2019: Metabolites
Andrew W Maksymiuk, Paramjit S Tappia, Daniel S Sitar, Parveen S Akhtar, Nazrina Khatun, Rahnuma Parveen, Rashiduzzaman Ahmed, Rashid Bux Ahmed, Brian Cheng, Gina Huang, Horacio Bach, Brett Hiebert, Bram Ramjiawan
Aim: Spermidine/spermine N1 -acetyltransferase (SSAT-1) plays a critical role in cell growth, proliferation and death, and is known to be activated in human cancer cells. Amantadine, a US FDA-approved antiviral drug, is a substrate for SSAT-1 and can be used to indirectly measure SSAT-1 activity because of its conversion to acetylamantadine (AA). This study was undertaken to further validate SSAT-1 activity in breast and lung cancer patients. Results: An increase in the urinary concentration of AA in lung and breast cancer patients was observed...
February 2019: Future Science OA
Osamu Yamaguchi
The autophagic machinery is a well-conserved degradation system in eukaryotes from yeast to mammals. Autophagy has been thought of as a nonselective degradation process in which cytoplasmic proteins and organelles are degraded by fusion with lysosome. Recent studies have revealed selective forms of autophagy, such as mitochondria-specific autophagy, termed "mitophagy". Research over the past decade has revealed that autophagy in cardiomyocytes plays a protective role, not only during hemodynamic stress but in homeostasis during aging...
February 28, 2019: Circulation Journal: Official Journal of the Japanese Circulation Society
Reshma V Patil, Kiran D Pawar
To extend our understanding of molecular mechanism of sex determination in agro-economically important, polygamodioecious tree Garcinia indica (Kokum), high-throughput, next-generation flower transcriptome sequencing (NGS), and comparative analyses were performed to investigate differentially expressed gene in bisexual, female, and male flowers. A total of 49414 unigenes in BS, 45944 unigenes in FL, and 49028 unigenes in ML flowers were annotated. KO annotations revealed that 25 functional categories were large number of genes which were annotated to 'signal transduction'...
March 2019: 3 Biotech
Hamed Soren Seifi, Barry J Shelp
Roles of the major polyamines (mPA), putrescine, spermidine, and spermine (Spm), in various developmental and physiological processes in plants have been well documented. Recently, there has been increasing focus on the link between mPA metabolism and defense response during plant-stress interactions. Empirical evidence is available for a unique role of Spm, distinct from the other mPA, in eliciting an effective defense response to (a)biotic stresses. Our understanding of the precise molecular mechanism(s) by which Spm modulates these defense mechanisms is limited...
2019: Frontiers in Plant Science
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