Yun-Peng Wang, Yu-Jie Zhao, Xiang-Liang Kong
Herein, we aimed to investigate the functions of ADAMTS6 in colon cancer and its potential mechanism. Based on the data acquired from TCGA database, we revealed that ADAMTS6 was highly expressed in colon cancer tissues, and high expression of ADAMTS6 predicted worse prognosis in patients with colon cancer. Moreover, qRT-PCR demonstrated that the levels of ADAMTS6 were higher in colon cancer cell lines (NCI-H508, Caco-2, CW-2 and HCT 116) than that in normal control cell line CCD-18Co. Functional experiments displayed that depletion of ADAMTS6 repressed NCI-H508 cell growth, invasion and migration whilst overexpression of ADAMTS6 facilitated Caco-2 cell growth, invasion and migration...
2020: Genetics and Molecular Biology
Bin Wang, Nina Ma, Xixi Zheng, Xiao Li, Xiao Ma, Jiexuan Hu, Bangwei Cao
Chemotherapy based on 5-fluorouracil (5-FU) is the standard approach for colon cancer treatment, and resistance to 5-FU is a significant obstacle in the clinical treatment of colon cancer. However, the mechanisms underlying 5-FU resistance in colon cancer cells remain largely unknown. This study aimed at determining whether 5-FU-resistant colon cancer cells undergo epithelial-mesenchymal transition (EMT) and apoptosis and the role of GDF15-a member of the transforming growth factor β /bone morphogenetic protein super family and a protein known to be involved in cancer progression-in the regulation of EMT and apoptosis of these cells, along with the underlying mechanisms...
2020: BioMed Research International
Syarinta Adenina, Melva Louisa, Vivian Soetikno, Wawaimuli Arozal, Septelia Inawati Wanandi
Purpose: This study was intended to find out the impact of alpha mangostin administration on the epithelial-mesenchymal transition (EMT) markers and TGF-β/Smad pathways in hepatocellular carcinoma Hep-G2 cells surviving sorafenib. Methods: Hepatocellular carcinoma HepG2 cells were treated with sorafenib 10 μM. Cells surviving sorafenib treatment (HepG2surv ) were then treated vehicle, sorafenib, alpha mangostin, or combination of sorafenib and alpha mangostin. Afterward, cells were observed for their morphology with an inverted microscope and counted for cell viability...
September 2020: Advanced Pharmaceutical Bulletin
Yongsen Jia, Xin Yan, Ying Cao, Wei Song, Guangji Zhang, Xueqin Hu
Background: To explore the activity of Modified Tongyou Decoction (MTD) against Eca109 esophageal cancer (EC) cell invasion and metastasis and to ascertain the mechanism of its anticancer activity during the epithelial-mesenchymal transition (EMT) as mediated by the HIF-1 α -Snail axis. Methods: Herbal compounds were prepared by ethanol extraction, and 6 herbs composing into MTD were dipped in water-free ethanol and filtered. The filtrate was collected and centrifuged...
2020: Evidence-based Complementary and Alternative Medicine: ECAM
Guijun He, Wenfeng Yao, Liang Li, Yang Wu, Guojian Feng, Li Chen
Background: LOXL1-AS1 is a long non-coding RNA (lncRNA) that plays crucial roles in various cancers. However, the functional role of LOXL1-AS1 in laryngocarcinoma remains unclear. Thus we planned to probe into the function and underlying mechanism of LOXL1-AS1 in laryngocarcinoma. Methods: Gene expression was evaluated in laryngocarcinoma cells using RT-qPCR. The ability of cell proliferation and migration was assessed by CCK8, colony formation, wound healing and transwell assays...
2020: Cancer Cell International
Hongwang Song, Xiaojun Fu, Chenxing Wu, Shouwei Li
Background: Glioblastoma multiforme (GBM) is the most malignant tumor in human brain, with highly heterogeneity among different patients. Age could function as an incidence and prognosis risk factor for many tumors. Method: A series of bioinformatic experiments were conducted to evaluate the differences of incidence, differential expressed genes, enriched pathways with the data from Surveillance, Epidemiology, and End Results (SEER) program, the cancer genome atlas (TCGA) and Chinese glioma genome atlas (CGGA) project...
2020: Cancer Cell International
Hui Wang, Xiaolong Cai, Longyang Ma
Background: The serious side effect of current conventional treatments for patients with metastatic colorectal cancer (CRC) highlights the requirement of an alternative treatment strategy. Natural compounds, such as curcumin, have been gained much attention due to its low toxicity and anti-tumor effect. Methods: qPCR and Western blot were used to measure the molecular changes induced by curcumin. Wound-healing assay and transwell assay were conducted to study the effect on cell migration and invasion...
2020: Cancer Management and Research
Xue Huang, Fujian Liu, Zhiyong Jiang, Hang Guan, Qiuhong Jia
Background: The cAMP response element-binding protein 1 (CREB1) was initiated as a potential target for cancer treatment. This research was conducted to probe the effect of CREB1 in the progression of gastric cancer (GC) and the molecules involved. Materials and Methods: CREB1 expression in GC tissues and cell lines (AGS and MKN-45) as well as that in normal tissues and in gastric mucosa cell line (GES-1) was detected. The correlation between CREB1 expression and prognosis of GC patients was determined...
2020: Cancer Management and Research
Bashan Zhang, Fei Li, Zinian Zhu, Aijiao Ding, Jintong Luo
Background: Hepatocellular carcinoma (HCC) is a common lethal malignant tumor worldwide. Circular RNAs (circRNAs) have been reported to affect the development of human cancers, including HCC. In this project, we aim to clarify the functional effect of circular CDR1as (circ_CDR1as) on HCC progression. Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) or Western blot is implemented to detect the expression of circ_CDR1as, microRNA (miR)-1287 and Raf-1 proto-oncogene, serine/threonine kinase (Raf1)...
2020: Cancer Management and Research
Shahrzad Soleymani Fard, Mansour Yazdanbod, Masoud Sotoudeh, Davood Bashash, Habibollah Mahmoodzadeh, Kioomars Saliminejad, Seyed Asadollah Mousavi, Seyed H Ghaffari, Kamran Alimoghaddam
Purpose: The clinical studies carried out in the last few decades unequivocally introduced activated androgen receptor (AR) as a pathogenic feature of human malignancies which not only endows cancer cells with survival advantage, but also may be exploited for anticancer interventions. Patients and Methods: In this study, we have investigated the expression profile of AR and EMT-related genes in fresh gastric cancer (GC), adjacent nontumor and normal gastric tissues, as well as the effect and molecular mechanisms of AR inhibition in GC cell lines...
2020: OncoTargets and Therapy
Yu Li, Gang Chen, Zhijian Han, Huijuan Cheng, Liang Qiao, Yumin Li
Background: Sorafenib is the standard first-line treatment for advanced hepatocellular carcinoma (HCC), even though acquired resistance to sorafenib has been found in many HCC patients, resulting in poor prognosis. Accumulating evidence demonstrates that liver cancer stem cells (LCSCs) contribute to sorafenib resistance in HCC. The inflammatory factor interleukin 6 (IL-6) plays a role in sorafenib resistance in HCC. However, the mechanism by which IL-6 in LCSCs is involved in the process of HCC sorafenib resistance remains elusive...
2020: OncoTargets and Therapy
Tao Zhang, Li Zhao, Taotao Zhang, Wangyu Wu, Jie Liu, Xian Wang, Yang Wan, Hao Geng, Xin Sun, Weiwei Qian, Dexin Yu
Background: So far, little research has been conducted regarding the underlying mechanism of renal carcinogenesis at molecular level. Epithelial-mesenchymal transition (EMT) exerts an important part during tumor genesis as well as the development through mitogen-activated protein kinase (MAPK) pathways. Therefore, we hypothesized that EMT could promote renal cell carcinoma (RCC) progression via the ERK5/AP-1pathway. Materials and Methods: The RCC cell lines were utilized to be the models with in vitro exposure to cigarette smoke extract (CSE)...
2020: OncoTargets and Therapy
Bing-Zheng Zhong, Qiang Wang, Feng Liu, Jia-Li He, Yi Xiong, Jie Cao
Objective: To explore the related influencing mechanism of miR-384 and miR-134-5p acting on Yin Yang 1 ( YY1 ) signaling transduction on the biological function of gastric cancer (GC) cells. Methods: miR-384, miR-134-5p and YY1 levels in human GC cell lines KATO III, MKN-45, SNU-1 and normal gastric cell line GES-1 were measured by polymerase chain reaction (PCR). Dual luciferase reporter (DLR) gene assay and Western blot (WB) were employed for correlation analysis between miR-384, miR-134-5p and YY1 ...
2020: OncoTargets and Therapy
Zhengtao Zhou, Xiaoqiang Liu, Yulei Li, Junhua Li, Wen Deng, Jian Zhong, Luyao Chen, Yu Li, Xiantao Zeng, Gongxian Wang, Jingyu Zhu, Bin Fu
Background: The tumor protein p53-inducible nuclear protein 2 (TP53INP2), an autophagy protein, is essential for autophagosome formation. The deregulation of autophagy is associated with multiple human diseases, including cancer. The present study aims to explore the role of TP53INP2 in bladder cancer. Materials and Methods: Quantitative real-time polymerase chain reaction was used to detect the mRNA level. Relative TP53INP2 protein expression was detected by immunohistochemistry and Western blot...
2020: OncoTargets and Therapy
Zhonglin Cai, Hongjun Li
Bladder cancer (BC) is the most common urinary system malignancy and is a serious threat to human health. Circular RNAs (circRNAs) are members of a newly defined class of noncoding RNAs (ncRNAs) that can regulate gene expression at the transcriptional or posttranscriptional level. Studies have shown that circRNAs are related to the clinicopathological characteristics, prognosis, and chemosensitivity of BC, and basic research has further confirmed that changes in the expression of circRNAs in BC are closely related to various tumor biological functions...
2020: OncoTargets and Therapy
Junbin Wang, Jin Gao, Qinnan Chen, Weiyan Zou, Fen Yang, Chenchen Wei, Zhaoxia Wang
Background: Long non-coding RNAs (lncRNAs) have been found to contribute to cisplatin resistance in several cancers; however, the role of lncRNA LINC01116 in cisplatin resistance remains unknown in non-small-cell lung cancer. This study aimed to examine the contribution of LINC01116 to cisplatin resistance in lung adenocarcinoma (LAD). Materials and Methods: Cisplatin-resistant A549/DDP cells were generated by treatment with cisplatin by dose escalation. LINC01116 expression was compared between A549 and A549/DDP cells, and between cisplatin-resistant and non-resistant LAD specimens...
2020: OncoTargets and Therapy
Dawei Cui, Kaijie Wang, Yan Liu, Junling Gao, Jianzhong Cui
Objective: Glioma has the highest incidence among the different tumor types within the nervous system, accounting for about 40% of them. Malignant glioma has a high invasion and metastasis rate, which leads to the poor prognosis of patients. By targeting specific genes, microRNAs serve as key regulators in the epithelial-mesenchymal transformation (EMT) process, which could provide new insights into the treatment of glioblastomas (GBM). The detailed molecular role that miR-623 plays in GBM still remains unclear...
2020: OncoTargets and Therapy
Mei Jiang, Qi Chen, Xiaoting Zhao, Yu Teng, Chenghong Yin, Wentao Yue
Background: PFTK1, a novel cyclin-dependent kinase, plays pivotal roles in tumorigenesis. Cell motility and invasiveness could be enhanced by PFTK1 in various tumors. However, the function of PFTK1 in NSCLC metastasis remains unclear. In this study, the potential role of PFTK1 in NSCLC metastasis was determined. Materials and Methods: In this study, the potential function of PFTK1 in lung cancer patients was analyzed with the Kaplan-Meier plotter database. RNA interference-mediated knockdown of PFTK1 was established in two NSCLC cell lines (H1299 and 95C) to explore the role of PFTK1 in NSCLC...
2020: OncoTargets and Therapy
Nan Wang, Honghong Yan, Di Wu, Zheng Zhao, Xiaoqi Chen, Qian Long, Changlin Zhang, Xiaohao Wang, Wuguo Deng, Xuekui Liu
Metastasis is the main cause of laryngeal cancer-related death; its molecular mechanism remains unknown. Here we identify protein arginine methyltransferase 5 (PRMT5) as a new metastasis-promoting factor in laryngeal carcinoma, and explore its underlying mechanism of action in regulating laryngeal cancer progression. We illustrated that PRMT5 expression was positively correlated with tumor stages, lymphatic metastasis, and unfavorable outcome. Functional assays revealed that PRMT5 promoted laryngeal carcinoma cell proliferation, migration, and invasive capacity in vitro, as well as lymph-node metastasis in vivo...
October 15, 2020: Cell Death & Disease
Xiang Deng, Peng Jiang, Jian Chen, Jianwei Li, Dajiang Li, Yu He, Yan Jiang, Yujun Zhang, Shuangnian Xu, Xi Li, Shuguang Wang, Feng Tian
GATA6 acts as an oncogene or tumour suppressor in different cancers. Previously, we found that aberrant expression of GATA6 promoted metastasis in cholangiocarcinoma (CCA). However, the mechanism by which GATA6 promotes metastasis in CCA is unclear. In the present study, we aimed to investigate the role of GATA6 in CCA cell epithelial-mesenchymal transition (EMT). Our results showed that GATA6 expression was positively associated with N-cadherin and vimentin expression but negatively associated with E-cadherin expression in 91 CCA samples...
October 15, 2020: Cell Death & Disease
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