Rivi Friedenberg, Leonid Kalichman, David Ezra, Oren Wacht, Deborah Alperovitch-Najenson
The aim of this article was to review the current knowledge relating to work-related musculoskeletal disorders (WRMDs) and non-fatal injuries in emergency medical technicians and paramedics (EMTs-Ps). A literature search was conducted in PubMed, Google Scholar, and Clinical Key. The annual prevalence of back pain ranged from 30% to 66%, and back injuries and contusions from 4% to 43%. Falls, slips, trips, and overexertion while lifting or carrying patients or instruments ranged from 10% to 56%, with overexertion being the most common injury...
October 19, 2020: Archives of Environmental & Occupational Health
Wenbiao Chen, Kefan Bi, Xujun Zhang, Jingjing Jiang, Hongyan Diao
Hepatocellular carcinoma (HCC) is an immune-related tumor, that the type and number of tumor-infiltrated immune cells can serve as biomarkers for the clinical application. In this study, we constructed the immune model for diagnostic and prognostic prediction of HCC based on the systematic bioinformatics analyses on the component of immune cells from large samples transcriptome. CIBERSORT analysis found that the component of immune cells between 513 HCC and 473 adjacent normal tissues was different. M0 macrophages and regulatory T cells were mainly enriched in tumor tissues, whereas the CD8+ T cell and activated CD4+ memory T cells were the most in normal tissues...
2020: Journal of translational autoimmunity
Xiaodong Yan, Dali Han, Zhiqiang Chen, Chao Han, Wei Dong, Li Han, Lei Zou, Jianbo Zhang, Yan Liu, Jie Chai
Background: Cancer stem cells (CSCs) play an important role in tumor invasion and metastasis. CD44 is the most commonly used marker of CSCs, with the potential to act as a determinant against the invasion and migration of CSCs and as the key factor in epithelial-mesenchymal transition (EMT)-like changes that occur in colorectal cancer (CRC). Runt-related transcription factor-2 (RUNX2) is a mesenchymal stem marker for cancer that is involved in stem cell biology and tumorigenesis. However, whether RUNX2 is involved in CSC and in inducing EMT-like changes in CRC remains uncertain, warranting further investigation...
2020: Cancer Cell International
Narges Aberuyi, Soheila Rahgozar, Elnaz Pourabutaleb, Kamran Ghaedi
Pediatric acute lymphoblastic leukemia (pALL) includes 75 % of childhood leukemias, and methotrexate (MTX) is one of the most effective chemotherapy agents prescribed for pALL treatment. The aim of this study was to establish and characterize an MTX-resistant tumor cell model in order to study the mechanism contributing to drug sensitivity loss in pALL. Parental CCRF-CEM cells were treated with a gradual increasing concentration of MTX from 5 nM to 1.28 μM. The resistant subline was then characterized according to the cellular morphology, cellular growth curves and specific mRNA expression changes associated with drug resistance in ALL...
October 15, 2020: Current Research in Translational Medicine
Mikihisa Takano, Junya Deguchi, Shunsuke Senoo, Miho Izumi, Masashi Kawami, Ryoko Yumoto
Quercetin is a flavonol that is known to have numerous beneficial biological effects such as an anti-fibrotic effect. Epithelial-mesenchymal transition (EMT) of alveolar type II epithelial cells is one of major causes of pulmonary fibrosis. However, the effect of quercetin on drug-induced EMT in alveolar type II cells is not known. In this study, we examined the effect of quercetin on bleomycin (BLM)-induced EMT using RLE/Abca3 cells having alveolar type II cell-like phenotype. BLM induced EMT-like morphological changes, downregulation of an epithelial marker E-cadherin, and upregulation of a mesenchymal marker α-smooth muscle actin in RLE/Abca3 cells...
August 12, 2020: Drug Metabolism and Pharmacokinetics
Li-Na Zhang, Di-Di Zhang, Lei Yang, Yu-Xuan Gu, Qiu-Ping Zuo, Hao-Yi Wang, Jia Xu, Dian-Xin Liu
Invasion and metastasis are the basic characteristics and important markers of malignant tumors, which are also the main cause of death in cancer patients. Epithelial-mesenchymal transition (EMT) is recognized as the first step of tumor invasion and metastasis. Many studies have demonstrated that cell fusion is a common phenomenon and plays a critical role in cancer development and progression. At present, cancer stem cell fusion has been considered as a new mechanism of cancer metastasis. Mesenchymal stromal/stem cell (MSC) is a kind of adult stem cells with high self-renewal ability and multi-differentiation potential, which is used as a very promising fusogenic candidate in the tumor microenvironment and has a crucial role in cancer progression...
July 13, 2020: FEBS Journal
Yangyang Guo, Yu Tong, Hengyue Zhu, Yanyi Xiao, Hangcheng Guo, Lumeng Shang, Wenjing Zheng, Shumei Ma, Xiaodong Liu, Yongheng Bai
Pancreatic ductal adenocarcinoma (PDA) is an aggressive type of malignant tumor with a poor prognosis and high mortality. Aberrant activation of hedgehog signaling plays a crucial role in the maintenance and progression of PDA. Here, we report that the dietary bioflavonoid quercetin has therapeutic potential for PDA by targeting sonic hedgehog (SHH) signaling. The effects of quercetin on the proliferation, apoptosis, migration, and invasion of pancreatic cancer cells (PCCs) and tumor growth and metastasis in PDA xenograft mouse models were evaluated...
October 17, 2020: Cell Biology and Toxicology
Leila Rouhi, Sirisha M Cheedipudi, Suet Nee Chen, Siyang Fan, Raffaella Lombardi, Xiaofan Chen, Cristian Coarfa, Matthew J Robertson, Priyatansh Gurha, Ali J Marian
AIMS: Arrhythmogenic cardiomyopathy (ACM) encompasses a genetically heterogeneous group of myocardial diseases whose manifestations are sudden cardiac death, cardiac arrhythmias, heart failure, and in a subset fibro-adipogenic infiltration of the myocardium. Mutations in the TMEM43 gene, encoding transmembrane protein 43 (TMEM43) are known to cause ACM. The purpose of the study was to gain insights into the molecular pathogenesis of ACM caused by TMEM43 haploinsufficiency. METHODS AND RESULTS: The Tmem43 gene was specifically deleted in cardiac myocytes by crossing the Myh6-Cre and floxed Tmem43 mice...
October 18, 2020: Cardiovascular Research
Andres M Cifuentes-Bernal, Vu Vh Pham, Xiaomei Li, Lin Liu, Jiuyong Li, Thuc Duy Le
MOTIVATION: microRNAs (miRNAs) are important gene regulators and they are involved in many biological processes, including cancer progression. Therefore, correctly identifying miRNA-mRNA interactions is a crucial task. To this end, a huge number of computational methods has been developed, but they mainly use the data at one snapshot and ignore the dynamics of a biological process. The recent development of single cell data and the booming of the exploration of cell trajectories using "pseudo-time" concept have inspired us to develop a pseudo-time based method to infer the miRNA-mRNA relationships characterising a biological process by taking into account the temporal aspect of the process...
October 18, 2020: Bioinformatics
Ruizan Shi, Linhong Liu, Fengge Wang, Yifan He, Yanan Niu, Chang Wang, Xuanping Zhang, Xiuli Zhang, Huifeng Zhang, Min Chen, Yan Wang
Induction of epithelial-mesenchymal transition (EMT) and cancer stem cell (CSC) characteristics underlie the development of metastasis, chemoresistance, and tumor recurrence in breast cancer. Downregulation of cytokeratin 18 (CK18) is a critical molecular event of EMT; however, its importance in the induction of EMT and CSC features has not been defined to date. This study aimed to investigate the biological significance and underlying molecular mechanisms of CK18 in inducing EMT phenotype and stemness properties of breast cancer cells...
October 15, 2020: Cellular Signalling
Jing Xu, Ping Zhang, Huajun Sun, Yang Liu
BACKGROUND: Long intergenic non-coding RNA 01094 (LINC01094) is probably a novel regulator in cancer biology. This study aimed to probe into the function and mechanism of LINC01094 in ovarian cancer (OC). METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) assay was utilized to measure LINC01094 and miR-577 expressions in OC tissues and cell lines. Western blot was used to examine the expressions of epithelial-mesenchymal transition (EMT)-related proteins, β-catenin, c-Myc and cyclin D1...
October 17, 2020: Journal of Ovarian Research
Xin Wang, Jian Wang, Jingjing Wu
HMGA2 (High Mobility Group AT-hook 2) has been reported to promote colorectal cancer (CRC) development by regulating the transcription of target genes. It participates in nearly all aspects of cellular processes, including cell transformation, proliferation, apoptosis, senescence, metastasis, epithelial-to-mesenchymal transition (EMT), DNA repair and stem cell self-renewal. In the past decades, a group of downstream targets and binding partners have been identified in a wide range of cancers. Our findings of HMGA2 as a key factor in the MDM2/p53, IL11/STAT3 and Wnt/β-catenin signaling pathways prompt us to summarize current advances in the functional and molecular basis of HMGA2 in CRC...
October 14, 2020: Translational Oncology
Qiong Wu, Jiali Ma, Jue Wei, Wenying Meng, Yugang Wang, Min Shi
Long non-coding RNAs (lncRNAs) are under active investigation in the development of cancers including gastric cancer (GC). Oncogenic autophagy is required for cancer cell survival. The present study aimed to investigate the regulatory role of lncRNA small nucleolar host gene 11 (SNHG11) in GC. We show that SNHG11 is upregulated in GC, and that its upregulation correlated with dismal patient outcomes. Functionally, SNHG11 aggravated oncogenic autophagy to facilitate cell proliferation, stemness, migration, invasion, and epithelial-to-mesenchymal transition (EMT) in GC...
October 14, 2020: Molecular Therapy
Ricardo Jorge Pais
Epithelial-to-Mesenchymal Transition (EMT) is a natural and reversible process involved in embryogenesis, wound healing and thought to participate in the process of metastasis. Multiple signals from the microenvironment have been reported to drive EMT. However, the tight control of this process on physiological scenarios and how it is disrupted during cancer progression is not fully understood. Here, we analysed a regulatory network of EMT accounting for 10 key microenvironment signals focusing on the impact of two cell contact signals on the reversibility of EMT and the stability of resulting phenotypes...
October 14, 2020: Bio Systems
Xian-Zi Yang, Xi-Min Chen, Li-Si Zeng, Jin Deng, Lei Ma, Chuan Jin, Ren Wang, Meng-He Wang, Yue-Feng Wen, Xiao-Liang Wu, Hui-Yun Wang, Shu-Zhong Cui
Many articles have reported that Rab1A was overexpressed in a variety of human cancers and involved in tumor progression and metastasis. However, the biological function and molecular mechanism of Rab1A in nasopharyngeal carcinoma (NPC) remained unknown until now. Here we found that Rab1A overexpression is a common event and was positively associated with distant metastasis and poor prognosis of NPC patients. Functionally, Rab1A depletion inhibited the migration and EMT phenotype of NPC cells, whereas Rab1A overexpression led to the opposite effect...
October 17, 2020: Aging
Dongsheng Sun, Yuqiao Zhao, Weina Wang, Canghai Guan, Zengtao Hu, Lang Liu, Xingming Jiang
This study was designed to illustrate the function and role of PCAT1 in CCA. The relative expression was confirmed by RT-qPCR and western blot. The biological function of PCAT1 was evaluated by CCK8, EdU, colony formation, wound healing, transwell, and subcutaneous tumor formation assays. Protein levels of EMT markers were measured by western blot. The binding relationship was predicted by JASPAR and starBase. The binding of YY1 to PCAT1 promoter was assessed by ChIP and luciferase reporter. The binding capacity between miR- 216a-3p and PCAT1 as well as BCL3 was assessed by luciferase reporter and AGO2-RIP assays...
October 1, 2020: Biological Chemistry
Akiyoshi Kasuga, Takashi Semba, Ryo Sato, Hiroyuki Nobusue, Eiji Sugihara, Hiromasa Takaishi, Takanori Kanai, Hideyuki Saya, Yoshimi Arima
Biliary tract cancer (BTC) arises from biliary epithelial cells (BECs) and includes intrahepatic cholangiocarcinoma (IHCC), gallbladder cancer (GC), and extrahepatic cholangiocarcinoma (EHCC). Although frequent KRAS mutations and epigenetic changes at the INK4A/ARF locus have been identified, the molecular pathogenesis of BTC is unclear and the development of corresponding anticancer agents remains inadequate. We isolated epithelial cell adhesion molecule (EpCAM)-positive BECs from the mouse intrahepatic bile duct, gallbladder, and extrahepatic bile duct and established organoids derived from these cells...
October 17, 2020: Cancer Science
Wolfgang Eberhardt, Kristina Haeussler, Usman Nasrullah, Josef Pfeilschifter
Colorectal cancer (CRC) is one of the most frequently diagnosed tumor in humans and one of the most common causes of cancer-related death worldwide. The pathogenesis of CRC follows a multistage process which together with somatic gene mutations is mainly attributed to the dysregulation of signaling pathways critically involved in the maintenance of homeostasis of epithelial integrity in the intestine. A growing number of studies has highlighted the critical impact of members of the tripartite motif (TRIM) protein family on most types of human malignancies including CRC...
October 13, 2020: International Journal of Molecular Sciences
Lulu Ni, Zhongjie Li, Xuelin Shi, Chao Yao, Jiangnan Sun, Min Ai, Shuangshuang Li, Yixiao Liu, Fei Xu, Yuetao Zhou, Jiangan Li, Liying Qiu
Lung cancer always ranks first in the number of cancer deaths every year, accounting for 18.4% of total cancer deaths in 2018. Metastasis is the main cause of death in lung cancer patients. The identification of bioactive components of traditional Chinese medicine is very important for the development of novel reagents against non-small cell lung cancer (NSCLC). Rosthorin A has originated from Rabdosia rosthornii (Diels) Hara which excerpts from 'Chinese materia medica', and is known to have 'clear heat phlegm' properties in the folk...
November 2020: Anti-cancer Drugs
Zhenxing Ding, Xu Wu, Yueguo Wang, Shuang Ji, Wenying Zhang, Jiaying Kang, Jiajia Li, Guanghe Fei
BACKGROUND: Oxidative stress plays a critical role in pulmonary fibrosis after acute lung injury (ALI), and epithelial-mesenchymal transition (EMT) events are involved in this process. The purpose of this study was to investigate the protective effects of melatonin, a natural antioxidant, on lipopolysaccharide (LPS)-induced EMT in human alveolar epithelial cells. METHODS: Human type II alveolar epithelial cell-derived A549 cells were incubated with LPS and melatonin alone or in combination for up to 24 h...
October 13, 2020: Biomedicine & Pharmacotherapy
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