A'wani Aziz Nurdalila, Maya Erna Natnan, Syarul Nataqain Baharum
Mass mortality resulting from bacterial infection poses a major problem in the grouper aquaculture industry. The purpose of this study was to profile the metabolites released in challenged fish and to reconstruct the metabolic pathways of brown marble grouper ( Epinephelus fuscoguttatus) in response to Vibrio vulnificus infection. Metabolite profiles from control and challenged treatment groups after feeding were determined using gas chromatography-mass spectrometry (GC-MS). Forty metabolites were identified from the GC-MS analysis...
December 2020: 3 Biotech
Xiaohan Yang, Xue Zhang, Yu Wang, Hui Shen, Ge Jiang, Jingquan Dong, Panpan Zhao, Song Gao
As an important foodborne pathogen, Vibrio vulnificus gives a significant threat to food safety and public health. Rapid and accurate detection methods for V. vulnificus are required to control its spread. The conventional detection methods are time-consuming and labor-intensive, while the polymerase chain reaction (PCR)- and quantitative PCR (qPCR)-based methods are limited because of their dependence on laboratory equipment. Nucleic acid isothermal amplification technologies have been applied to develop simpler assays...
2020: Frontiers in Microbiology
Yi Sun, Yu-Ze Lin, Zeng-Gan Chen
Vibrio vulnificus is a deadly marine pathogen that can cause necrotizing fasciitis, septic shock, and even death in severe cases. The relatively low incidence and atypical early-stage symptoms may hinder many physicians from carrying out surgical intervention effectively, thus leading to an increase of mortality in infected patients. This article reported a patient who developed necrotizing fasciitis and septic shock after the exposure to freshwater shrimp stabbed on the limb. By reviewing and analyzing previous studies, it was found out that the timing of surgery could have a significant impact on the patients for their necrotizing fasciitis caused by Vibrio vulnificus infection...
November 23, 2020: International Journal of Lower Extremity Wounds
T N R Srinivas, P Sudharani, Swarnaprava Behera, S Dutta, G Sampath Kumar, Y Sathibabu, G Geethika, C Swagata, P Pratim Kandar
To examine the influence of anthropogenic activities on the marine ecosystem around the Andaman and Nicobar Islands, a study was conducted to investigate the abundance of heterotrophic, indicator and pathogenic bacteria during the monsoon season. We noticed the higher abundance of heterotrophic, indicator (Escherichia coli and Enterococcus faecalis) and pathogenic bacterial counts (Aeromonas hydrophila, Enterobacter aerogens, Escherichia coli strain O157:H7, Shigella dysenteriae and Vibrio parahaemolyticus) near the 10o channel, which is the principal route to reach Andaman mainland...
November 18, 2020: Marine Pollution Bulletin
Sehwang Jeong, Jungwook Kim
CmoB utilizes carboxy-S-adenosyl-l-methionine (CxSAM) to carry out unusual carboxymethyl transfer to form 5-carboxymethoxyuridine (cmo5 U) of several tRNA species in Gram-negative bacteria. In this report, we present three X-ray crystal structures of CmoB from Vibrio vulnificus representing different states in the course of the reaction pathway; i.e., apo-, substrate-bound, and product-bound forms. Especially, the crystal structure of apo-CmoB unveils a novel open state of the enzyme, capturing unprecedented conformational dynamics around the substrate-binding site...
November 16, 2020: Biochemical and Biophysical Research Communications
Yuan Yuan, Zihan Feng, Jinglin Wang
The Vibrio vulnificus ( V. vulnificus ) hemolysin (VVH) is a pore-forming cholesterol-dependent cytolysin (CDC). Although there has been some debate surrounding the in vivo virulence effects of the VVH, it is becoming increasingly clear that it drives different cellular outcomes and is involved in the pathogenesis of V. vulnificus . This minireview outlines recent advances in our understanding of the regulation of vvhA gene expression, the biological activity of the VVH and its role in pathogenesis. An in-depth examination of the role of the VVH in V...
2020: Frontiers in Immunology
Asmaa Sadat, Heba El-Sherbiny, Amira Zakaria, Hazem Ramadan, Amal Awad
AIMS: Infection of seafood with pathogenic species of the genus Vibrio causes human food-borne illnesses. This study was executed to examine the antimicrobial-resistance phenotypes, biofilm-forming capability, and virulence-associated genes of Vibrio from fish and shellfishes. METHODS AND RESULTS: Three hundred fresh water and marine fish and shellfish samples were collected from wet markets and supermarkets in Mansoura, Egypt. Bacteriological examination and PCR amplification identified 92 Vibrio spp...
November 13, 2020: Journal of Applied Microbiology
Laura Cutugno, Jennifer Mc Cafferty, Jan Pané-Farré, Conor O'Byrne, Aoife Boyd
Rifampicin is a broad-spectrum antibiotic that binds to the bacterial RNA polymerase (RNAP), compromising DNA transcription. Rifampicin resistance is common in several microorganisms and it is typically caused by point mutations in the gene encoding the β subunit of RNA polymerase, rpoB . Different rpoB mutations are responsible for various levels of rifampicin resistance and for a range of secondary effects. rpoB mutations conferring rifampicin resistance have been shown to be responsible for severe effects on transcription, cell fitness, bacterial stress response and virulence...
November 13, 2020: Microbiology
Hector Carmona-Salido, Naiel Bisharat, Carmen Amaro
Vibrio vulnificus is a zoonotic pathogen that is spreading worldwide due to global warming. Lineage 3 (L3; formerly biotype 3) includes the strains of the species with the unique ability to cause fish farm-linked outbreaks of septicaemia. The L3-strains emerged recently and are particularly virulent and difficult to identify. Here we describe a new developed PCR method based on a comparative genomic study useful for both rapid identification and epidemiological studies of this interesting emerging group. The comparative genomic analysis also revealed the presence of a genetic duplication in the L3 strains that could be related to the unique ability of this lineage to produce septicemia outbreaks...
November 4, 2020: Journal of Clinical Microbiology
Sharmin Quazi Bonny, M A Motalib Hossain, Syed Muhammad Kamal Uddin, Thiruchelvi Pulingam, Suresh Sagadevan, Mohd Rafie Johan
Vibrio parahaemolyticus , Vibrio cholerae and Vibrio vulnificus are the most significant aquatic pathogens of the genera Vibrio , account for most Vibrio -associated outbreaks worldwide. Rapid identification of these pathogens is of great importance for disease surveillance, outbreak investigations and food safety maintenance. Traditional culture dependent methods are time-consuming and labor-intensive whereas culture-independent polymerase chain reaction (PCR) based assays are reliable, consistent, rapid and reproducible...
November 4, 2020: Critical Reviews in Food Science and Nutrition
Majel Victoria P Miles, Kaitlin M Alexander, Kelsea C Wright, Ryan P Miller, Lindsay J Lindsey, Jared L Burkett, Steven A Kahn
No abstract text is available yet for this article.
October 30, 2020: American Surgeon
Xian-Hui Huang, Yao Ma, Meng-Meng Zheng, Na Chen, Mei-Na Hu, Liu-Ying Wu, Yi Zheng, Yong-Liang Lou, Dan-Li Xie
The marine bacterium Vibrio vulnificus causes potentially fatal bloodstream infections, typically in patients with chronic liver diseases. The inflammatory response and anti-bacterial function of phagocytes are crucial for limiting bacterial infection in the human hosts. How V. vulnificus affects macrophages after phagocytosis is unclear. In this report, we found that the bactericidal activity of macrophages to internalize V. vulnificus was dependent on mammalian target of rapamycin (mTOR) and NOD-like receptor (NLR) family pyrin domain containing 3 (NLRP3) interaction...
2020: Frontiers in Cell and Developmental Biology
Seung Min Kim, Jongmin Park, Myun Soo Kim, Heebum Song, Ala Jo, Hankum Park, Tae Sung Kim, Sang Ho Choi, Seung Bum Park
An antivirulence agent against Vibrio vulnificus named quoromycin (QM) was discovered by a phenotype-based elastase inhibitor screening. Using the fluorescence difference in two-dimensional gel electrophoresis (FITGE) approach, SmcR, a quorum-sensing master regulator and homologue of LuxR, was identified as the target protein of QM. We confirmed that the direct binding of QM to SmcR inhibits the quorum-sensing signaling pathway by controlling the DNA-binding affinity of SmcR and thus effectively alleviates the virulence of V...
October 21, 2020: ACS Infectious Diseases
Sharafaldin Al-Musawi, Salim Albukhaty, Hassan Al-Karagoly, Ghassan M Sulaiman, Mona S Alwahibi, Yaser Hassan Dewir, Dina A Soliman, Humaira Rizwana
This paper describes the preparation, characterization, and evaluation of honey/tripolyphosphate (TPP)/chitosan (HTCs) nanofibers loaded with capsaicin derived from the natural extract of hot pepper ( Capsicum annuum L .) and loaded with gold nanoparticles (AuNPs) as biocompatible antimicrobial nanofibrous wound bandages in topical skin treatments. The capsaicin and AuNPs were packed within HTCs in HTCs-capsaicin, HTCs-AuNP, and HTCs-AuNPs/capsaicin nanofibrous mats. In vitro antibacterial testing against Pasteurella multocida, Klebsiella rhinoscleromatis, Staphylococcus pyogenes, and Vibrio vulnificus was conducted in comparison with difloxacin and chloramphenicol antibiotics...
October 17, 2020: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Stéphanie Copin, Julia Mougin, Virginie Raguenet, Annick Robert-Pillot, Graziella Midelet, Thierry Grard, Maryse Bonnin-Jusserand
Vibrio spp., ubiquitous in the aquatic ecosystem, are bacteria of interest because of their involvement in human health, causing gastroenteritis after ingestion of seafood, as well as their role in vibriosis leading to severe losses in aquaculture production. Their ability to enter a viable but non-culturable (VBNC) state under stressful environmental conditions may lead to underestimation of the Vibrio population by traditional microbiological enumeration methods. As a result, using molecular methods in combination with EMA or PMA allows the detection of viable (VBNC and culturable viable) cells...
October 15, 2020: Letters in Applied Microbiology
Victoria Lynn Pruente, William C Walton, Jessica L Jones
During routine handling, cultured oysters are removed from the water and exposed to elevated temperatures, causing growth of Vibrio vulnificus and V. parahaemolyticus within. Farmers can resubmerse oysters in the water, allowing elevated Vibrio spp. levels to return to ambient levels within the oysters. Previous resubmersion research is limited to one aquaculture gear type during studies performed from June-September. This study aims to expand existing knowledge on the recovery times needed for elevated Vibrio levels in handled oysters from two common gear types (adjustable longline system [ALS] and OysterGro system [OG]) during early and mid-summer periods...
October 9, 2020: Journal of Food Protection
Victoria L Pruente, Jessica L Jones, Todd D Steury, William C Walton
Routine handling of oysters is a common industry practice for off-bottom oyster aquaculture, which aims to produce a high-quality oyster. These practices expose oysters to elevated temperatures and interrupt filter feeding, which can increase Vibrio vulnificus and V. parahaemolyticus levels within the oyster. The resubmersion of oysters after exposure to conditions where the time-temperature controls are exceeded is as an effective mitigation strategy to allow elevated levels of Vibrio spp. to "recover", or return to ambient levels, prior to harvest...
September 26, 2020: International Journal of Food Microbiology
Swanandi Pote, Sangita Kachhap, Nicholas J Mank, Leily Daneshian, Vincent Klapper, Sarah Pye, Amy K Arnette, Linda S Shimizu, Tomasz Borowski, Maksymilian Chruszcz
BACKGROUND: The products of the lysine biosynthesis pathway, meso-diaminopimelate and lysine, are essential for bacterial survival. This paper focuses on the structural and mechanistic characterization of 4-hydroxy-tetrahydrodipicolinate reductase (DapB), which is one of enzymes from the lysine biosynthesis pathway. DapB catalyzes the conversion of (2S, 4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate (HTPA) to 2,3,4,5-tetrahydrodipicolinate in an NADH/NADPH dependent reaction. Genes coding for DapBs were identified as essential for many pathogenic bacteria, and therefore DapB is an interesting new target for the development of antibiotics...
September 24, 2020: Biochimica et Biophysica Acta. General Subjects
Salina Parveen, John Jacobs, Gulnihal Ozbay, Lathadevi K Chintapenta, Esam Almuhaideb, Joan Meredith, Sylvia Ossai, Amanda Abbott, Arquette Grant, Kathy Brohawn, Paulinus Chigbu, Gary P Richards
Oyster and seawater samples were collected from five sites in the Chesapeake Bay, MD, and three sites in the Delaware Bay, DE, from May-October 2016 and 2017. Abundances and detection frequencies for total and pathogenic Vibrio parahaemolyticus and Vibrio vulnificus were compared using the standard Most Probable Number-PCR (MPN-PCR) assay and a direct plating (DP) method on CHROMagar™ Vibrio for total ( tlh+ ) and pathogenic ( tdh+ and trh+ ) V. parahaemolyticus genes, and total ( vvhA ) and pathogenic ( vcgC ) genes for V...
September 25, 2020: Applied and Environmental Microbiology
Qingyao Wang, Songzhe Fu, Qian Yang, Jingwei Hao, Can Zhou, Ying Liu
The estuary is the ecological niche of pathogenic Vibrio spp. as it provides abundant organic and inorganic nutrients from seawater and rivers. However, little is known about the ecology of these Vibrio species in the inland brackish water area. In this study, their co-occurrence and relationships to key environmental constraints (salinity and temperature) in the Hun-Tai River of China were examined using the most probable number polymerase chain reaction (MPN-PCR) approach. We hereby report 2-year continuous surveillance based on six water indices of the Hun-Tai River...
September 17, 2020: International Journal of Environmental Research and Public Health
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