Aaron Bodansky, David Jl Yu, Alysa N Rallistan, Muge Kalaycioglu, Jim Boonyaratanakornkit, Damian J Green, Jordan Gauthier, Cameron J Turtle, Kelsey C Zorn, Brian O'Donovan, Caleigh Mandel-Brehm, James Asaki, Hannah Kortbawi, Andrew F Kung, Elze Rackaityte, Chung-Yu Wang, Aditi Saxena, Kimberly de Dios, Gianvito Masi, Richard J Nowak, Kevin C O'Connor, Hao Li, Valentina E Diaz, Rowan Saloner, Kaitlin B Casaletto, Eva Q Gontrum, Brandon J Chan, Joel H Kramer, Michael R Wilson, Paul J Utz, Joshua A Hill, Shaun W Jackson, Mark S Anderson, Joseph L DeRisi
Given the global surge in autoimmune diseases, it is critical to evaluate emerging therapeutic interventions. Despite numerous new targeted immunomodulatory therapies, comprehensive approaches to apply and evaluate the effects of these treatments longitudinally are lacking. Here, we leveraged advances in programmable-phage immunoprecipitation (PhIP-Seq) methodology to explore the modulation, or lack thereof, of autoantibody profiles, proteome-wide, in both health and disease. Using a custom set of over 730,000 human derived peptides, we demonstrated that each individual, regardless of disease state, possesses a distinct and complex constellation of autoreactive antibodies...
May 16, 2024: Journal of Clinical Investigation