Gianluca Rugna, Elena Carra, Federica Bergamini, Giuliana Franzini, Silvia Faccini, Antonietta Gattuso, Marina Morganti, Deborah Baldi, Simona Naldi, Andrea Serraino, Silvia Piva, Giuseppe Merialdi, Federica Giacometti
Listeria monocytogenes contamination in raw pork and ready to eat foods is an important food safety concern, also for the increasing detection of antimicrobial-resistant isolates. Data on L. monocytogenes occurrence, persistence, distribution and genetic characterization in two different plants, namely in continuum from slaughtered pigs, environment and unfinished products (fresh hams) were observed by one-year monitoring and were integrated with their antimicrobial resistance patterns. A total of 98 samples out of the overall 1131 (8...
October 9, 2020: International Journal of Food Microbiology
Miriam Mateos, Marta Hernández-García, Rosa Del Campo, Laura Martínez-García, Desirée Gijón, María Isabel Morosini, Patricia Ruiz-Garbajosa, Rafael Cantón
Carbapenemase production is constantly increasing among different Enterobacterales species. We analyzed the microbiological characteristics and population structure of all carbapenemase-producing Enterobacter spp. (CP-Ent) isolates recovered at the Ramón y Cajal Hospital between 2005 and 2018. Overall, 178 CP-Ent isolates (60.7% colonization, 39.3% clinical) were recovered from 165 hospitalized patients (165/176, 93.7%; medical [102/165], surgical [34/165], and intensive care unit [29/165] areas), emergency unit (4/176, 2...
October 22, 2020: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Israel Labastida, Linda A Mercado, Sarai Rojas, Beatriz Barrera, Margarita Beltrán, M Aurora Armienta, René H Lara, Rosa M Luna
The dispersion of mine tailings affects ecosystems due to their high content of potentially toxic elements. Environmental risk increases when the soil impacted by tailings is used for agriculture; this use may result in health impacts. This study analyzes the feasibility of remediating a calcareous soil (used for maize cultivation) polluted with lead in the semiarid zone of Zimapán, México, by using EDTA as an extractant. Total geoavailable and bioaccessible concentrations in the gastric and intestinal phases were determined to evaluate lead availability and health risk...
October 22, 2020: Environmental Geochemistry and Health
Joanna Wyczarska-Kokot, Anna Lempart-Rapacewicz, Mariusz Dudziak, Edyta Łaskawiec
Recommendations regarding disinfection by-products (DBPs) in pool waters consider the content of trihalomethanes (THMs) and combined chlorine (CC) as indicators of DBPs based on which the health risk for swimmers and staff of pool facility can be determined. However, the content of DBPs in swimming pools depends on many factors. In this paper, the influence of selected factors (physicochemical parameters of water and technological parameters) on the content of THMs and CC in pool water was determined. During the 6-month period, 9 pools of various functions were analyzed...
October 22, 2020: Environmental Monitoring and Assessment
Kanishka Kamathewatta, Rhys Bushell, Fannana Rafa, Glenn Browning, Helen Billman-Jacobe, Marc Marenda
BACKGROUND: Hospital intensive care units (ICUs) are known reservoirs of multidrug resistant nosocomial bacteria. Targeted environmental monitoring of these organisms in health care facilities can strengthen infection control procedures. A routine surveillance of extended spectrum beta-lactamase (ESBL) producers in a large Australian veterinary teaching hospital detected the opportunistic pathogen Enterobacter hormaechei in a hand washing sink of the ICU. The organism persisted for several weeks, despite two disinfection attempts...
October 21, 2020: Antimicrobial Resistance and Infection Control
Irina Kirsh, Yuliya Frolova, Olga Beznaeva, Olga Bannikova, Marina Gubanova, Isabella Tveritnikova, Valentina Romanova, Yulia Filinskaya
Particular attention is paid to biodegradable materials from the environmental point of view and antimicrobial materials that ensure the microbiological safety of packaged products. The aim of the work was to study the properties of the composition, based on biodegradable polybutylene adipate terephthalate (PBAT) and the antimicrobial additive-birch bark extract (BBE). Test samples of materials were obtained on the laboratory extruder by extrusion with ultrasonic treatment of the melt. The concentration of the antimicrobial additive in the polymer matrix was 1 wt %...
October 19, 2020: Polymers
Paulina Cholewińska, Magdalena Wołoszyńska, Marta Michalak, Katarzyna Czyż, Witold Rant, Marzena Janczak
Studies carried out so far have indicated the effect of the microbiome on the composition of ruminant products. Recent studies have shown that not only diet, but also genetic factors can affect the microbiological composition of the digestive system. The aim of the study was to determine the differences in the levels of selected bacterial phyla in terms of breed differences. Three sheep breeds, i.e., Olkuska, Romanov, and old-type Polish Merino, differing in their use (meat-wool, meat, prolificacy) and country of breed origin were included in the study...
October 16, 2020: Animals: An Open Access Journal From MDPI
J Cameron Thrash
Principal challenges in environmental microbiology include determining the functions of specific microorganisms, when and where those functions change, and why. Unveiling this information for creatures on the micron scale is exquisitely difficult, but culturing microorganisms offers a sturdy experimental foundation for such queries. Unfortunately, bringing microbes into culture presents its own set of challenges, which, combined with their vast diversity, means most microorganisms remain uncultivated (Schloss et al...
October 18, 2020: Environmental Microbiology Reports
Kelly J Hidalgo, Isabel N Sierra-Garcia, Bruna M Dellagnezze, Valéria Maia de Oliveira
Petroleum is a very complex and diverse organic mixture. Its composition depends on reservoir location and in situ conditions and changes once crude oil is spilled into the environment, making the characteristics associated with every spill unique. Polycyclic aromatic hydrocarbons (PAHs) are common components of the crude oil and constitute a group of persistent organic pollutants. Due to their highly hydrophobic, and their low solubility tend to accumulate in soil and sediment. The process by which oil is sourced and made available for use is referred to as the oil supply chain and involves three parts: (1) upstream, (2) midstream and (3) downstream activities...
2020: Frontiers in Microbiology
Mirel Glevitzky, Lotfi Aleya, Mihaela Laura Vică, Gabriela-Alina Dumitrel, Mariana Avram, Delia Mirela Tit, Maria Popa, Viorel-Cristian Popa, Tapan Behl, Simona Bungau
Microbial contamination and damaging effects due to environmental factors (temperature, humidity) of old books is an extremely important issue in the heritage preserving field, being a great threat to this unique cultural and literary treasure. This paper analysed the indoor environmental conditions, the microbiological characteristics of the air and the condition itself of 27 clerical books (from eighteenth to nineteenth centuries), randomly chosen from the library of Bistrița Monastery, Romania. The analysis revealed that most of the books were contaminated and damaged by moulds and yeasts...
October 17, 2020: Environmental Science and Pollution Research International
Victoria Grießmeier, Jan Wienhöfer, Harald Horn, Johannes Gescher
Environmental contamination with fertilizers is threatening biodiversity in many ecosystems due to nitrate-based eutrophication. One opportunity for a cost-efficient nitrate elimination are denitrification beds in which a microbial community thrives under anoxic conditions with polymeric plant material as a carbon and an electron source. Incoming nitrate is used as electron acceptor and reduced to molecular nitrogen. Projects realizing denitrification beds in field scale are sparse and robust data on their efficiency throughout the year mostly not available...
September 30, 2020: Water Research
Rebecca Co, Laura A Hug
Improved sequencing technologies and the maturation of metagenomic approaches allow identification of gene variants with potential industrial applications, including cellulases. Cellulase identification from metagenomic environmental surveys is complicated by inconsistent nomenclature and multiple categorization systems. Here, we summarize the current classification and nomenclature systems, with recommendations for improvements to these systems. Addressing the described issues will strengthen annotation of cellulose-active enzymes from environmental sequence datasets - a rapidly growing resource in environmental and applied microbiology...
October 16, 2020: Applied and Environmental Microbiology
Sara Ciulli, Enrico Volpe, Rubina Sirri, Giorgia Tura, Francesca Errani, Gianpiero Zamperin, Anna Toffan, Marina Silvi, Andrea Renzi, Miriam Abbadi, Lorena Biasini, Tobia Pretto, Pietro Emmanuele, Antonio Casalini, Giuseppe Sarli, Patrizia Serratore, Oliviero Mordenti, Luciana Mandrioli
This investigation focused on an episode of chronic mortality observed in juvenile Huso huso sturgeons. The examined subjects underwent pathological, microbiological, molecular, and chemical investigations. Grossly severe body shape deformities, epaxial muscle softening, and multifocal ulcerative dermatitis were the main observed findings. The more constant histopathologic findings were moderate to severe rarefaction and disorganization of the lymphohematopoietic lymphoid tissues, myofiber degeneration, atrophy and interstitial edema of skeletal epaxial muscles, and degeneration and atrophy of the gangliar neurons close to the myofibers...
October 13, 2020: Animals: An Open Access Journal From MDPI
Shashi B Kumar, Shanvanth R Arnipalli, Ouliana Ziouzenkova
Antibiotics have been used as essential therapeutics for nearly 100 years and, increasingly, as a preventive agent in the agricultural and animal industry. Continuous use and misuse of antibiotics have provoked the development of antibiotic resistant bacteria that progressively increased mortality from multidrug-resistant bacterial infections, thereby posing a tremendous threat to public health. The goal of our review is to advance the understanding of mechanisms of dissemination and the development of antibiotic resistance genes in the context of nutrition and related clinical, agricultural, veterinary, and environmental settings...
October 13, 2020: Antibiotics
Guoxia Liu, Nooreen Mamode Ally, Daminee Devi Dooly, Yin Li, Kamleshwar Boodhoo, Hudaa Neetoo
BACKGROUND: Animal manure frequently harbors pathogenic microorganisms such as Salmonella spp and diarrheagenic Escherichia coli. A defined microbial consortium such as Effective Microorganisms (EM) can potentially be used as a biocontrol against manure-borne human pathogens such as Salmonella and pathogenic E. coli. The objective of the study was to investigate the efficacy of EM to decontaminate cattle manure. RESULTS: EM was first characterized by enumeration and identification of lactic acid bacteria (LAB), yeasts, actinomycetes and phototrophic bacteria (PB)...
October 15, 2020: Journal of the Science of Food and Agriculture
Nazek Jisr, Ghassan Younes, Khaled El Omari, Monzer Hamze, Carol Sukhn, Mohammad H El-Dakdouki
The present study aimed at evaluating the levels of microbiological contamination, total petroleum hydrocarbons (TPHs), and heavy metals (As, Cd, Hg, and Pb) in the edible tissues of commonly consumed fish (8 species) collected from the marine area of Tripoli, Northern Lebanon. Total coliform levels in all sampled fish, and Escherichia coli levels in Liza ramada only, exceeded the permissible limits set by FAO/WHO 2002. Staphylococcus aureus counts were within the recommended thresholds, while sulfate-reducing bacteria levels were the highest in fish of the genus Liza...
October 16, 2020: Environmental Monitoring and Assessment
A Saravanan, P Senthil Kumar, S Karishma, Dai-Viet N Vo, S Jeevanantham, P R Yaashikaa, Cynthia Susan George
Nanotechnology has become one of the emerging multi-disciplinary fields receiving universal attention and playing a substantial role in agriculture, environment and pharmacology. In spite of various techniques employed for nanoparticle synthesis such as laser ablation, mechanical milling, spinning and chemical deposition, usage of hazardous chemicals and expensiveness of the process makes it unsuitable for the continuous production. Hence the necessity of sustainable, economic and environment friendly approach development have increased in recent years...
October 9, 2020: Chemosphere
Stéphanie Copin, Julia Mougin, Virginie Raguenet, Annick Robert-Pillot, Graziella Midelet, Thierry Grard, Maryse Bonnin-Jusserand
Vibrio spp., ubiquitous in the aquatic ecosystem, are bacteria of interest because of their involvement in human health, causing gastroenteritis after ingestion of seafood, as well as their role in vibriosis leading to severe losses in aquaculture production. Their ability to enter a viable but non-culturable (VBNC) state under stressful environmental conditions may lead to underestimation of the Vibrio population by traditional microbiological enumeration methods. As a result, using molecular methods in combination with EMA or PMA allows the detection of viable (VBNC and culturable viable) cells...
October 15, 2020: Letters in Applied Microbiology
Adriano Sofo, Hazem S Elshafie, Ippolito Camele
Plants are affected by soil environments to the same extent that they affect soil functioning through interactions between environmental and genetic factors. Here, five plant species (broad bean, pea, cabbage, fennel, and olive) grown under controlled pot conditions were tested for their ability to differently stimulate the degradation of standard litter. Litter, soil C and N contents were measured for evaluating chemical changes due to plant presence, while soil microbial abundance was evaluated to assess if it had a positive or negative catalyzing influence on litter decomposition...
October 10, 2020: Plants (Basel, Switzerland)
P Sarati Das, G Gagnon-Turcotte, K Ouazaa, K Bouzid, S Nazila Hosseini, E Bharucha, D Tremblay, S Moineau, Y Messaddeq, J Corbeil, B Gosselin
This paper presents the EcoChip 2, an autonomous multimodal bio-environmental sensor platform for the monitoring of microorganisms in the northern habitat. The EcoChip 2 prototype includes an array of 96-wells for the continuous monitoring of microbiological growth through a multichannel electrochemical impedance analyzer circuit. In addition, the platform includes luminosity, humidity, temperature sensors and monitoring. The developed electronic board uses an ultra-low-power microcontroller unit, a custom power management unit, a low-power wireless ISM-2...
July 2020: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
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