Salima Benbarche, Jose Mario Bello Pineda, Laura Baquero Galvis, Jeetayu Biswas, Bo Liu, Eric Wang, Qian Zhang, Simon J Hogg, Kadeen Lyttle, Ariana Dahi, Alexander M Lewis, Martina Sarchi, Jahan Rahman, Nina Fox, Yuxi Ai, Sanjoy Mehta, Ralph Garippa, Juliana Ortiz-Pacheco, Zhuoning Li, Mara Monetti, Robert F Stanley, Sergei Doulatov, Robert K Bradley, Omar Abdel-Wahab
Mutations in the RNA splicing factor gene SF3B1 are common across hematologic and solid cancers and result in widespread alterations in splicing, yet there is currently no therapeutic means to correct this mis-splicing. Here, we utilize synthetic introns uniquely responsive to mutant SF3B1 to identify trans factors required for aberrant mutant SF3B1 splicing activity. This revealed the G-patch domain-containing protein GPATCH8 as required for mutant SF3B1-induced splicing alterations and impaired hematopoiesis...
April 21, 2024: Molecular Cell