Jean-Michel Ané, Julien Lévy, Philippe Thoquet, Olga Kulikova, Françoise de Billy, Varma Penmetsa, Dong-Jin Kim, Frédéric Debellé, Charles Rosenberg, Douglas R Cook, Ton Bisseling, Thierry Huguet, Jean Dénarié
The DMI1, DMI2, and DMI3 genes of Medicago truncatula, which are required for both nodulation and mycorrhization, control early steps of Nod factor signal transduction. Here, we have used diverse approaches to pave the way for the map-based cloning of these genes. Molecular amplification fragment length polymorphism markers linked to the three genes were identified by bulked segregant analysis. Integration of these markers into the general genetic map of M. truncatula revealed that DMI1, DMI2, and DMI3 are located on linkage groups 2, 5, and 8, respectively...
November 2002: Molecular Plant-microbe Interactions: MPMI