Enrique Pola-Sánchez, Karen Magdalena Hernández-Martínez, Rafael Pérez-Estrada, Nelly Sélem-Mójica, June Simpson, María Jazmín Abraham-Juárez, Alfredo Herrera-Estrella, José Manuel Villalobos-Escobedo
RNA sequencing (RNA-seq) has emerged as a powerful tool for assessing genome-wide gene expression, revolutionizing various fields of biology. However, analyzing large RNA-seq datasets can be challenging, especially for students or researchers lacking bioinformatics experience. To address these challenges, we present a comprehensive guide to provide step-by-step workflows for analyzing RNA-seq data, from raw reads to functional enrichment analysis, starting with considerations for experimental design. This is designed to aid students and researchers working with any organism, irrespective of whether an assembled genome is available...
May 2024: Current protocols