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REVIEW
Sajal Gupta, Rakesh Sharma, Ashok Agarwal, Florence Boitrelle, Renata Finelli, Ala'a Farkouh, Ramadan Saleh, Taha Abo-Almagd Abdel-Meguid, Murat Gül, Birute Zilaitiene, Edmund Ko, Amarnath Rambhatla, Armand Zini, Kristian Leisegang, Shinnosuke Kuroda, Ralf Henkel, Rossella Cannarella, Ayad Palani, Chak-Lam Cho, Christopher C K Ho, Daniel Suslik Zylbersztejn, Edoardo Pescatori, Eric Chung, Fotios Dimitriadis, Germar-Michael Pinggera, Gian Maria Busetto, Giancarlo Balercia, Gianmaria Salvio, Giovanni M Colpi, Gökhan Çeker, Hisanori Taniguchi, Hussein Kandil, Hyun Jun Park, Israel Maldonado Rosas, Jean de la Rosette, Joao Paulo Greco Cardoso, Jonathan Ramsay, Juan Alvarez, Juan Manuel Corral Molina, Kareim Khalafalla, Kasonde Bowa, Kelton Tremellen, Evangelini Evgeni, Lucia Rocco, Marcelo Gabriel Rodriguez Peña, Marjan Sabbaghian, Marlon Martinez, Mohamed Arafa, Mohamed S Al-Marhoon, Nicholas Tadros, Nicolas Garrido, Osvaldo Rajmil, Pallav Sengupta, Paraskevi Vogiatzi, Parviz Kavoussi, Ponco Birowo, Raghavender Kosgi, Saleem Bani-Hani, Sava Micic, Sijo Parekattil, Sunil Jindal, Tan V Le, Taymour Mostafa, Tuncay Toprak, Yoshiharu Morimoto, Vineet Malhotra, Azin Aghamajidi, Damayanthi Durairajanayagam, Rupin Shah
Antisperm antibodies (ASA), as a cause of male infertility, have been detected in infertile males as early as 1954. Multiple causes of ASA production have been identified, and they are due to an abnormal exposure of mature germ cells to the immune system. ASA testing (with mixed anti-globulin reaction, and immunobead binding test) was described in the WHO manual 5th edition and is most recently listed among the extended semen tests in the WHO manual 6th edition. The relationship between ASA and infertility is somewhat complex...
January 1, 2022: World Journal of Men's Health
#22
JOURNAL ARTICLE
Bhawana Shrestha, Kathleen Vincent, Alison Schaefer, Yong Zhu, Gracie Vargas, Massoud Motamedi, Kelsi Swope, Josh Morton, Carrie Simpson, Henry Pham, Miles B Brennan, Michael H Pauly, Larry Zeitlin, Barry Bratcher, Kevin J Whaley, Thomas R Moench, Samuel K Lai
Nonhormonal products for on-demand contraception are a global health technology gap; this unmet need motivated us to pursue the use of sperm-binding monoclonal antibodies to enable effective on-demand contraception. Here, using the cGMP-compliant Nicotiana -expression system, we produced an ultrapotent sperm-binding IgG antibody possessing 6 Fab arms per molecule that bind a well-established contraceptive antigen target, CD52g. We term this hexavalent antibody "Fab-IgG-Fab" (FIF). The Nicotiana -produced FIF had at least 10-fold greater sperm-agglutination potency and kinetics than the parent IgG, while preserving Fc-mediated trapping of individual spermatozoa in mucus...
November 30, 2021: Proceedings of the National Academy of Sciences of the United States of America
#23
JOURNAL ARTICLE
Tse-En Wang, Ling-Yu Yeh, Robert Kuo-Kuang Lee, Chung-Hao Lu, Tsung-Hsien Yang, Yu-Wen Kuo, Radhika Joshi, Pei-Shiue Tsai, Sheng-Hsiang Li
A flavin-dependent enzyme quiescin Q6 sulfhydryl oxidase 1 (QSOX1) catalyzes the oxidation of thiol groups into disulfide bonds. QSOX1 is prominently expressed in the seminal plasma. However, its role in male reproduction is elusive. Here, we purified the secreted form of QSOX1, i.e., QSOX1c, from mouse seminal vesicle secretions and revealed for the first time its function involved in sperm physiology. Exogenous addition of QSOX1c time-dependently promoted the in vitro aggregation of thiol-rich, oxidative stressed, and apoptotic mouse and human sperm cells...
October 22, 2021: IScience
#24
JOURNAL ARTICLE
Ping Li, Kehong Wei, Xia He, Lu Zhang, Zhaoxia Liu, Jing Wei, Xiaomei Chen, Hong Wei, Tingtao Chen
Background: The vaginal microbiota is associated with the health of the female reproductive system and the offspring. Lactobacillus crispatus belongs to one of the most important vaginal probiotics, while its role in the agglutination and immobilization of human sperm, fertility, and offspring health is unclear. Methods: Adherence assays, sperm motility assays, and Ca2+ -detecting assays were used to analyze the adherence properties and sperm motility of L. crispatus Lcr-MH175, attenuated Salmonella typhimurium VNP20009, engineered S...
2021: Frontiers in Cell and Developmental Biology
#25
JOURNAL ARTICLE
Sérgio Ricardo Maiolino, Adriana Cortez, Helio Langoni, Rogério Giuffrida, Joeleni Rosa Dos Santos, Geraldo de Nardi Júnior, Gustavo Henrique Batista Lara, Rodrigo Garcia Motta, Marcelo George Mungai Chacur, Fábio Morato Monteiro, Marcos Bryan Heinemann, Antônio Francisco de Souza Filho, Lorrayne de Souza Araújo Martins, Thaís Spessotto Bello, Márcio Garcia Ribeiro
The present study investigated the serum microscopic agglutination test (MAT) among 203 bovine bulls with reproduction by natural means, without apparent signs of orchitis or inflammation of accessory reproductive glands. Simultaneously, the semen of all bulls was subjected to sperm viability analysis and PCR based on the 16S rRNA gene. PCR-positive results of semen samples were confirmed by sequencing. A modified seminal plasma agglutination (MSPA) test, replacing the blood serum of all bulls in the MAT with seminal plasma was performed as well...
August 23, 2021: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
#26
JOURNAL ARTICLE
Bhawana Shrestha, Alison Schaefer, Yong Zhu, Jamal Saada, Timothy M Jacobs, Elizabeth C Chavez, Stuart S Omsted, Carlos A Cruz-Teran, Gabriela Baldeon Vaca, Kathleen Vincent, Thomas R Moench, Samuel K Lai
Many women risk unintended pregnancy because of medical contraindications or dissatisfaction with contraceptive methods, including real and perceived side effects associated with the use of exogenous hormones. We pursued direct vaginal delivery of sperm-binding monoclonal antibodies (mAbs) that can limit progressive sperm motility in the female reproductive tract as a strategy for effective nonhormonal contraception. Here, motivated by the greater agglutination potencies of polyvalent immunoglobulins but the bioprocessing ease and stability of immunoglobulin G (IgG), we engineered a panel of sperm-binding IgGs with 6 to 10 antigen-binding fragments (Fabs), isolated from a healthy immune-infertile woman against a unique surface antigen universally present on human sperm...
August 11, 2021: Science Translational Medicine
#27
REVIEW
Konstantinos Pantos, Sokratis Grigoriadis, Penelope Tomara, Ioanna Louka, Evangelos Maziotis, Agni Pantou, Nikolaos Nitsos, Terpsithea Vaxevanoglou, Georgia Kokkali, Ashok Agarwal, Konstantinos Sfakianoudis, Mara Simopoulou
There is a great body of evidence suggesting that in both humans and animal models the microRNA-34/449 (miR-34/449) family plays a crucial role for normal testicular functionality as well as for successful spermatogenesis, regulating spermatozoa maturation and functionality. This review and critical analysis aims to summarize the potential mechanisms via which miR-34/449 dysregulation could lead to male infertility. Existing data indicate that miR-34/449 family members regulate ciliogenesis in the efferent ductules epithelium...
2021: Frontiers in Endocrinology
#28
JOURNAL ARTICLE
G J Brown, J O Nöthling, K G M De Cramer
An interesting pattern of tail-in, head out sperm agglutination was identified in a Brucella canis seronegative subfertile dog. Centrifuged seminal plasma from this dog could induce a similar pattern of agglutination in six other dogs, but not in ejaculates from a single stallion and two rams. The agglutination pattern was short-lived, and appeared to depend on motility of spermatozoa, although intensity of agglutination may have been affected by concentration of agglutinating factor.
July 17, 2021: Reproduction in Domestic Animals
#29
JOURNAL ARTICLE
Gabriela Baldeon-Vaca, Jai G Marathe, Joseph A Politch, Emilie Mausser, Jeffrey Pudney, James Doud, Ellena Nador, Larry Zeitlin, Michael Pauly, Thomas R Moench, Miles Brennan, Kevin J Whaley, Deborah J Anderson
BACKGROUND: Approximately 40% of human pregnancies are unintended, indicating a need for more acceptable effective contraception methods. New antibody production systems make it possible to manufacture reagent-grade human monoclonal antibodies (mAbs) for clinical use. We used the Nicotiana platform to produce a human antisperm mAb and tested its efficacy for on-demand topical contraception. METHODS: Heavy and light chain variable region DNA sequences of a human IgM antisperm antibody derived from an infertile woman were inserted with human IgG1 constant region sequences into an agrobacterium and transfected into Nicotiana benthamiana...
July 2021: EBioMedicine
#30
JOURNAL ARTICLE
Kazim R Chohan, Crystal A Kling, Timothy K Byler
Objective: To report a rare case of schistosomiasis observed during semen evaluation. Design: Case report. Setting: University hospital. Patients: A 30-year-old man referred for semen analysis. Interventionss: None. Main Outcome Measuress: Poor sperm motility and viability. Results: The patient produced 9.8 mL of brown colored semen with a bad odor...
March 2021: F&S reports
#31
JOURNAL ARTICLE
Mohammed Hoque, Danny Chen, Rex A Hess, Feng-Qian Li, Ken-Ichi Takemaru
Cilia are evolutionarily conserved microtubule-based structures that perform diverse biological functions. Cilia are assembled on basal bodies and anchored to the plasma membrane via distal appendages. In the male reproductive tract, multicilia in efferent ducts (EDs) move in a whip-like motion to prevent sperm agglutination. Previously, we demonstrated that the distal appendage protein CEP164 recruits Chibby1 (Cby1) to basal bodies to facilitate basal body docking and ciliogenesis. Mice lacking CEP164 in multiciliated cells (MCCs) (FoxJ1-Cre;CEP164fl/fl) show a significant loss of multicilia in the trachea, oviduct, and ependyma...
July 8, 2021: Reproduction
#32
JOURNAL ARTICLE
Kohei Umezu, Shouhei Kurata, Kenshiro Hara, Kentaro Tanemura
Sperm head-to-head agglutination is a well-known known phenomenon in mammalian and non-mammalian species. Although several factors have been reported to induce sperm agglutination, information on the trigger and process of sperm detachment from the agglutination is scarce. Since hyperactivated motility is involved in bovine sperm detachment from the oviduct, we focused on caffeine, a well-known hyperactivation inducer, and aimed to determine the role of caffeine in sperm detachment from agglutination. Agglutination rate of bovine sperm was significantly decreased upon incubation with caffeine following pre-incubation without caffeine...
May 25, 2021: Biochemical and Biophysical Research Communications
#33
JOURNAL ARTICLE
Isabel Ortiz, Matheus Felix, Hélène Resende, Luisa Ramírez-Agámez, Charles C Love, Katrin Hinrichs
PURPOSE: To define the effect of sperm agglutination, associated with incubation under capacitating conditions, on accuracy of membrane assessment via flow cytometry and to develop methods to mitigate that effect. METHODS: Sperm motility was measured by CASA. Sperm were stained with PI-PSA or a novel method, LD-PSA, using fixable live/dead stain and cell dissociation treatment, before flow-cytometric analysis. Using LD-PSA, acrosome reaction and plasma membrane status were determined in equine sperm treated with 10 μm A23187 for 10 min, followed by 0, 1, or 2 h incubation in capacitating conditions...
May 15, 2021: Journal of Assisted Reproduction and Genetics
#34
JOURNAL ARTICLE
Nani Sari Murni, Indra Gusti Mansur, Ichramsyah A Rachman, Mohamad Sadikin
OBJECTIVE: Infertility in women could be a result of an excessive production of antisperm antibody (ASA). Paternal lymphocyte immunization (PLI) could decrease the ASA levels, but the mechanism is still unclear. The aim of this study was to analyse the impact of a PLI-induced ASA decline on regulatory T-cell populations and serum interleukin-10 (IL-10) levels in women with unexplained infertility. METHODS: Samples were obtained from patients who came to Sayyidah Mother and Child Hospital in Jakarta from July 2018 to April 2019 with infertility problems...
February 2021: JPMA. the Journal of the Pakistan Medical Association
#35
JOURNAL ARTICLE
Jin Kobayashi, Aya Sasaki, Asuka Watanabe, Kenichi Komine, Toshinobu Kuroishi, Naokazu Takada, Hiroshi Sasada, Eimei Sato
The purpose of this study was to investigate effects of addition of lactoferrin on characteristics and functions of bovine epididymal, ejaculated, and frozen-thawed sperm. The addition of lactoferrin was significantly (p < .05) effective on increasing values of progressive motility, straightness, and linearity in caput epididymal sperm and values of motility in cauda epididymal sperm. When ejaculated sperm were incubated in capacitation medium, percentages of motile and progressively motile sperm decreased largely within the first period of 30 min, followed by only minor changes...
January 2021: Animal Science Journal
#36
JOURNAL ARTICLE
Jonathan R Cowart, Danielle M Collins, Antonio A Mignucci-Giannoni, Tamara Alejandro-Zayas, Antonio L Rivera-Guzman, Iskande V Larkin
Limited information is available regarding male reproductive physiology in West Indian manatees ( Trichechus manatus ). Currently, only basic ultrastructural and morphometric descriptions of the spermatozoon exist; however, there are no reports evaluating any seminal characteristics in this species. Therefore, the aim of the study was to fill current gaps in knowledge regarding semen parameters in West Indian manatees by collecting and characterizing multiple ejaculate samples from a single, adult West Indian manatee...
2020: Frontiers in Veterinary Science
#37
JOURNAL ARTICLE
O V Bukharin, M D Kuzmin, N B Perunova, E V Ivanova, A V Bekpergenova, T A Bondarenko
BACKGROUND: One of the leading causes of the occurrence of chronic bacterial prostatitis (CBP) in men is infection, microecological disorders of the urogenital tract and cytokine-mediated mechanisms of inflammation of the prostate gland, which actualizes a comprehensive study of the clinical and bacteriological features of CBP from the perspective of a symbiotic approach in the framework of a new scientific field - "infectious symbiology". OBJECTIVE: to study the characteristics of spermogram, microbiota, and the cytokine profile in men with chronic bacterial prostatitis (CBP) and CBP complicated by infertility...
November 2020: Urologii︠a︡
#38
JOURNAL ARTICLE
Santhanahalli Siddalingappa Archana, Sellappan Selvaraju, Arunachalam Arangasamy, Balakrishnan Binsila, Divakar Swathi, Laxman Ramya, Arumugam Kumaresan, Balaganur Krishnappa
The objective of this study was to assess the influence of spermatozoa surface antigenic proteins on the functional competence of bovine neat and frozen-thawed semen. The breeding bulls (n = 38) were screened for seminal antigenic levels in neat semen based on the agglutination titrations with anti-sperm antibody (ASA). Bulls having high (n = 8) and low (n = 7) antigenic levels were selected and spermatozoa functional parameters were analyzed in neat and frozen-thawed semen samples. In neat semen, kinematics such as straightness (73...
October 24, 2020: Theriogenology
#39
JOURNAL ARTICLE
Bhawana Shrestha, Alison Schaefer, Elizabeth C Chavez, Alexander J Kopp, Timothy M Jacobs, Thomas R Moench, Samuel K Lai
Multivalent antibodies such as sIgA can crosslink motile entities such as sperm and bacteria, creating agglomerates that are too large to permeate the dense mucin matrix in mucus, a process commonly referred to as immune exclusion. Unfortunately, sIgA remains challenging to produce in large quantities, and easily aggregates, which prevented their use in clinical applications. To develop sIgA-like tetravalent antibodies that are stable and can be easily produced in large quantities, we designed two IgGs possessing 4 identical Fab domains, with the Fabs arranged either in serial or in the diametrically opposite orientation...
September 13, 2020: Acta Biomaterialia
#40
JOURNAL ARTICLE
Hala I Al-Daghistani
Objective: The effectiveness of Staphylococcus protein A (SPA) in improving the penetration ability of sperm and reducing antisperm antibody (ASA) titers in immunologically infertile males was evaluated. Methods: Seminal fluid samples were obtained from 15 infertile men, and ASA titers were assessed with the latex agglutination test. Identification of immunoglobulin (Ig) classes and characterization of the antigens involved in the immune response were performed using indirect immunofluorescence...
August 31, 2020: Clinical and Experimental Reproductive Medicine
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