Sperm agglutination

Aditi Chauhan, Vijay Prabha
The present study was carried out to assess the contraceptive efficacy of sperm agglutinating factor (SAF) isolated from Serratia marcescens , in male Balb/c mice. Mice were administered via an intratesticular route with different concentrations of SAF, viz. , 10, 50, 100, 200, or 400  µ g, in the right testis only which served as a test while the left side served as control except otherwise stated. Mice were sacrificed on day 3, 7, 14, 21, 30, 45, 60, and 90 after administration, and results in terms of change in body weight, seminal parameters, tissue somatic indices (TSI), hematological parameters, serum level of testosterone, lipid peroxidation, and histology were studied...
2019: BioMed Research International
Neeraj Chandra Pant, Ravinder Singh, Vijaya Gupta, Aditi Chauhan, Ravimohan Mavuduru, Vijay Prabha, Prince Sharma
BACKGROUND: Voluntary control of fertility is of paramount importance to the modern society. But since the contraceptive methods available for women have their limitations such as urinary tract infections, allergies, cervical erosion and discomfort, a desperate need exists to develop safe methods. Vaginal contraceptives may be the answer to this problem, as these are the oldest ways of fertility regulation, practiced over the centuries. With minimal systemic involvement, these are also the safest...
October 27, 2019: Reproductive Biology and Endocrinology: RB&E
Joaquín Cordero-Martínez, Juan Carlos Flores-Alonso, Charmina Aguirre-Alvarado, Norma Angélica Oviedo de Anda, Verónica Alcántara-Farfán, Carlos García-Pérez, Karla Fernanda Bermúdez-Ruiz, Guadalupe Elizabeth Jiménez-Gutiérrez, Lorena Rodríguez-Páez
ETHNOPHARMACOLOGY RELEVANCE: In traditional Mexican medicine Echeveria gibbiflora DC has been used as a vaginal post coital rinse to prevent pregnancy. The aqueous crude extract (OBACE) induced sperm immobilization/agglutination and a hypotonic-like effect, likely attributed to the high concentrations of calcium bis-(hydrogen-1-malate) hexahydrate [Ca2+ (C4 H5 O5 )2 •6H2 O]. Further studies showed that OBACE impedes the increase of [Ca2+ ]i during capacitation. AIM OF THE STUDY: This study aims to show the antifertility effect of OBACE, as well as its possible mechanism of action...
October 23, 2019: Journal of Ethnopharmacology
LaTasha B Craig, Richard S Brush, Michael T Sullivan, Michael T Zavy, Martin-Paul Agbaga, Robert E Anderson
PURPOSE: To determine if levels of very long chain polyunsaturated fatty acids (VLC-PUFA; ≥ 28 carbons;4-6 double bonds) in human sperm correlate with sperm quantity and quality as determined by a complete semen analysis. METHODS: Ejaculates from 70 men underwent a complete semen analysis, which included volume, count, motility, progression, agglutination, viscosity, morphology, and pH. For lipid analysis, sperm were pelleted to remove the semen. Lipids were extracted from the cell pellet and methyl esters of total lipids analyzed by gas chromatography...
May 9, 2019: Journal of Assisted Reproduction and Genetics
Berta Terré, Michael Lewis, Gabriel Gil-Gómez, Zhiyuan Han, Lu Hao, Mònica Aguilera, Neus Prats, Sudipto Roy, Haotian Zhao, Travis H Stracker
GEMC1 and MCIDAS are Geminin family proteins that transcriptionally activate E2F4/5-target genes during multiciliogenesis, including FoxJ1 and Ccno Male mice lacking Gemc1, Mcidas or Ccno were found to be infertile, but the origin of this defect has remained unclear. Here we show that all three genes are necessary for the generation of functional multiciliated cells in the efferent ducts that are required for spermatozoa to enter the epididymis. In mice mutant for Gemc1, Mcidas or Ccno, we observed a similar spectrum of phenotypes, including thinning of the seminiferous tubule epithelia, dilation of the rete testes, sperm agglutinations in the efferent ducts and lack of spermatozoa in the epididymis (azoospermia)...
April 1, 2019: Development
Monika Kowalska-Góralska, Katarzyna Dziewulska, Mateusz Kulasza
Nanoproducts are being increasingly used in various industrial products, leading to a greater risk of water pollution through their discharge into environment as production byproducts. Increased levels of environmental pollution with nanoproducts pose a threat to all living organisms. Nanopollutants may have toxic effects on gametes and fertilization process in species with external fertilization, thereby reducing effectiveness of reproduction or greatly impairing it. The objective of the present study was to determine the effect of copper nanoparticles (Cu and CuO) and copper ions (CuSO4 ·5H2 O) on the spermatozoa motility of sea trout and compare their harmful effects...
March 20, 2019: Aquatic Toxicology
Garrett K Berger, Luriel I Smith-Harrison, Jay I Sandlow
Agglutination is a finding noted in semen analyses (SAs) that often causes confusion as to its significance. While some have attributed agglutination to antisperm antibodies (ASAs), there are other causes as well, such as genital tract infection and ascorbic acid deficiency. Additionally, it is known that patients with ASAs often have risk factors such as a history of scrotal trauma or surgery. Therefore, we sought to determine the prevalence of agglutination in our patient population and correlate it with these risk factors, regardless of the presence/absence of ASAs...
June 2019: Andrologia
Shuiqiao Yuan, Yue Liu, Hongying Peng, Chong Tang, Grant W Hennig, Zhuqing Wang, Li Wang, Tian Yu, Rachel Klukovich, Ying Zhang, Huili Zheng, Chen Xu, Jingwen Wu, Rex A Hess, Wei Yan
Cilia are cell-surface, microtubule-based organelles that project into extracellular space. Motile cilia are conserved throughout eukaryotes, and their beat induces the flow of fluid, relative to cell surfaces. In mammals, the coordinated beat of motile cilia provides highly specialized functions associated with the movement of luminal contents, as seen with metachronal waves transporting mucus in the respiratory tract. Motile cilia are also present in the male and female reproductive tracts. In the female, wave-like motions of oviductal cilia transport oocytes and embryos toward the uterus...
February 26, 2019: Proceedings of the National Academy of Sciences of the United States of America
Levente Várkonyi, Zoltán Bokor, József Molnár, Ferenc Fodor, Zsolt Szári, Árpád Ferincz, Ádám Staszny, Levente Zete Láng, Balázs Csorbai, Béla Urbányi, Gergely Bernáth
In our study, a traditionally used (Grayling, already used in cyprinid species) and a newly tested (Pike) extender was tested to avoid sperm agglutination phenomenon following thawing during carp sperm cryopreservation. A large-scale (elevated volume of sperm) freezing method in a controlled-rate freezer using 5 mL straw and 10 mL cryotube was also systematically established. In all experiments, the sperm cryopreserved in using Grayling extender (except only 1 sample) showed an agglutination phenomenon (damaged and intact cells adhered to each other) after thawing where Pike extender resulted the regular cell suspension...
November 28, 2018: Reproduction in Domestic Animals
Nicolas Gatimel, Jessika Moreau, François Isus, Nathalie Moinard, Jean Parinaud, Roger D Leandri
RESEARCH QUESTION: Anti-sperm antibodies (ASA) have been shown to reduce male fertility but consensus about the precise situations in which tests should be carried out are lacking. In infertility investigations, should the mixed antiglobulin reaction (MAR) test be a first-line test? Should it be carried out systematically before assisted reproductive technology (ART)? What are the risk factors for ASA? DESIGN: All infertile patients (n = 1364) were tested with SpermMar (modified MAR test) between July 2013 and June 2017...
December 2018: Reproductive Biomedicine Online
Aylin Esmailkhani, Mohammad Taghi Akhi, Javid Sadeghi, Behrooz Niknafs, Abed Zahedi Bialvaei, Laya Farzadi, Nooshafarin Safadel
Background: Staphylococcus aureus is an infrequent, but one of the most successful bacteria that associated with infertility and are able to spermatozoa immobilization and agglutination. Objective: The aim of present study was to determine the frequency of S. aureus in semen obtained from infertile male patients in northwest Iran. Materials and Methods: Seminal fluids of 100 infertile men were evaluated. Standard semen examination was done according to World Health Organization guidelines...
July 2018: International Journal of Reproductive Biomedicine (Yazd, Iran)
Martina Rocco, Rafael Betarelli, Anna Placci, Josep M Fernández-Novell, Marcella Spinaci, Adriana Casao, Teresa Muiño-Blanco, José A Cebrián-Pérez, Alejandro Peña, Teresa Rigau, Sergi Bonet, Miriam Castillo-Martín, Marc Yeste, Joan E Rodríguez-Gil
This work sought to address the effects of melatonin during in vitro capacitation (IVC) and progesterone-induced acrosome exocytosis (IVAE) in boar spermatozoa. With this purpose, two different experiments were set. In the first one, IVC and IVAE were induced in the absence or presence of melatonin, which was added either at the start of IVC or upon triggering the IVAE with progesterone. Different parameters were evaluated, including intracellular levels of peroxides and superoxides, free cysteine radicals and distribution of specific lectins...
June 1, 2018: Andrology
A Lyons, F Narciandi, E Donnellan, J Romero-Aguirregomezcorta, C O' Farrelly, P Lonergan, K G Meade, S Fair
Primate β-defensin 126 regulates the ability of spermatozoa to bind to oviductal epithelial cells invitro. Bovine β-defensin 126 (BBD126) exhibits preferential expression in the cauda epididymis of the bull, but there have been few studies on its functional role in cattle. The aim of the present study was to examine the role of BBD126 in bull sperm binding to bovine oviductal epithelial cell (BOEC) explants. BBD126 has been shown to be highly resistant to the standard methods of dissociation used in other species and, as a result, corpus epididymal spermatozoa, which have not been exposed to the protein, were used to study the functional role of BBD126...
May 18, 2018: Reproduction, Fertility, and Development
Arpita Bhoumik, Sudipta Saha, Pavan V Payghan, Prasanta Ghosh, Sandhya Rekha Dungdung
Motility of spermatozoa is a crucial factor for determining semen quality. Here we report motility inhibitory factor (MIF-II) from goat epididymal plasma, revealing its structure, function, localization and motility inhibitory pathway. Structural characterization with MALDI revealed novelty of this protein while circular dichroism data confirmed its alpha helical nature. Higher dilutions of MIF-II antibody increased cauda sperm motility and induced immature/immotile caput sperm motility as tested microscopically...
September 2018: International Journal of Biological Macromolecules
Stanley Ifeanyi Ugwu, Kogiku Shiba, Kazuo Inaba, Masaya Morita
Identification of seminal proteins provides a means of investigating their roles. Despite their importance in the study of protein function, such as regulation of sperm motility, it is difficult to select candidates from the large number of proteins. Analyzing the rate of molecular evolution is a useful strategy for selecting candidates, and expressing the protein allows the examination of its function. In the present study, we investigated seminal plasma proteins of the cichlid Oreochromis mossambicus, which exhibits a unique mode of fertilization and a rapidly evolving gene that encodes a seminal plasma protein, zona-pellucida 3-like (ZP3-like), which does not belong to the same molecular family as other ZPs...
April 2018: Zoological Science
Somayeh Bahrami, Hossein Hamidinejat, Seyed Reza Fatemi-Tabatabaei, Saeed Sardarifar
Neospora is one of the protozoans that can infect the male and female's reproduction system. Despite the existence of N. caninum in the genitalia, its effect on sperm characteristics was not studied yet. Therefore, the aim of this study was to investigate the influence of natural neosporosis on the sperm parameters of bulls. Using 30 bulls with neosporosis diagnosed by modified agglutination test and enzyme-linked immunosorbent assay (ELISA) and 15 healthy bulls, some sperm parameters such as sperm concentration, viability, motility, and morphology were studied and compared...
January 2018: Tropical Animal Health and Production
Dalia Kheirallah, Lamia El-Samad, Naglaa Fahmi, Wafaa Osman
Ultrastructure alterations in spermiogenesis of the ground beetle, Blaps sulcata (Coleoptera: Tenebrionidae) were studied in normal adult males and in two male groups irradiated by gamma rays of 3 and 16 μSv/h dose rate. Ultrastructure examination of irradiated sperms revealed many alterations both in the head and in the flagellum regions of mature sperm. Alterations of the head region included nuclei with uncondensed chromatin materials and irregular nuclear envelope. Abnormal flagella contained malformed mitochondrial derivatives and damaged axonemes in addition to the absence of accessory bodies...
September 2017: Environmental Science and Pollution Research International
G Nardi Júnior, J Megid, L A Mathias, L Paulin, A F Vicente, A Cortez, F J P Listoni, G H B Lara, R G Motta, M G M Chacur, F M Monteiro, M G Ribeiro
Brucellosis remains as a major infectious disease of domestic animals and is considered a re-emerging zoonosis in several countries. B. abortus infections in bulls are related to reproductive tract infections, although infected animals show transient serological titers or nonreactor status. Thus, diagnosis of bovine brucellosis based exclusively on serological tests probably underestimates B. abortus infections in bulls. In this scenario, three hundred thirty-five serum samples from reproductively mature bovine bulls were subjected simultaneously to standard serodiagnosis using the rose Bengal test (RBT), 2-mercaptoethanol (2-ME), complement fixation (CFT), and fluorescence polarization assay (FPA)...
July 2017: Biologicals: Journal of the International Association of Biological Standardization
J M Teijeiro, P E Marini, M J Bragado, L J Garcia-Marin
Male germ cells undergo different processes within the female reproductive tract to successfully fertilize the oocyte. These processes are triggered by different extracellular stimuli leading to activation of protein phosphorylation. Protein kinase C (PKC) is a key regulatory enzyme in signal transduction mechanisms involved in many cellular processes. Studies in boar sperm demonstrated a role for PKC in the intracellular signaling involved in motility and cellular volume regulation. Experiments using phorbol 12-myristate 13-acetate (PMA) showed increases in the Serine/Threonine phosphorylation of substrates downstream of PKC in boar sperm...
March 2017: Andrology
Kuppusamy Chenniappan, Kadarkari Murugan
CONTEXT: Ionidium suffruticosum (L.) Ging (Violaceae) is an important medicinal plant widely used as a herbal traditional medicine in Ayurveda for the treatment of infertility. Currently, little pharmacological information is available on its male fertility properties following prolonged use. OBJECTIVE: To investigate I. suffruticosum leaf extracts for male fertility parameters. MATERIALS AND METHODS: The ethanol lyophilized fraction was administered orally on carbendazim-induced sub-fertility rats (250 mg/kg body weight for 28 days)...
December 2017: Pharmaceutical Biology
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