William Bain, Brian Ahn, Hernán F Peñaloza, Christi L McElheny, Nathanial Tolman, Rick van der Geest, Shekina Gonzalez-Ferrer, Nathalie Chen, Xiaojing An, Ria Hosuru, Mohammadreza Tabary, Erin Papke, Naina Kohli, Nauman Farooq, William Bachman, Tolani F Olonisakin, Zeyu Xiong, Marissa P Griffith, Mara Sullivan, Jonathan Franks, Mustapha M Mustapha, Alina Iovleva, Tomeka Suber, Robert Q Shanks, Viviana P Ferreira, Donna B Stolz, Daria Van Tyne, Yohei Doi, Janet S Lee
BACKGROUND: Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-Kp) bloodstream infections are associated with high mortality. We studied clinical bloodstream KPC-Kp isolates to investigate mechanisms of resistance to complement, a key host defense against bloodstream infection. METHODS: We tested growth of KPC-Kp isolates in human serum. In serial isolates from a single patient, we performed whole genome sequencing and tested for complement resistance and binding by mixing study, direct ELISA, flow cytometry, and electron microscopy...
January 25, 2024: Journal of Infectious Diseases