Rong Liu, Wen-Jun Cheng, Feng Ye, Yao-Dan Zhang, Qin-Ping Zhong, Hui-Fen Dong, Hong-Bin Tang, Hong Jiang
Schistosomiasis, caused by the parasitic flatworms called schistosomes, remains one of the most prevailing parasitic diseases in the world. The prodigious oviposition of female worms after maturity is the main driver of pathology due to infection, yet our understanding about the regulation of development and reproduction of schistosomes is limited. Here, we comparatively profiled the transcriptome of Schistosoma japonicum recovered from SCID and BALB/c mice, which were collected 35 days post-infection, when prominent morphological abnormalities could be observed in schistosomes from SCID mice, by performing RNA-seq analysis...
2020: Frontiers in Microbiology
Bai Dai, Fei Hao, Teng Xu, Bing Zhu, Li-Qing Ren, Xiao-Yu Han, Dong-Jun Liu
Increasing cashmere yield is one of the important goals of cashmere goat breeding. To achieve this goal, we screened the key genes that can improve cashmere performance. In this study, we used the RNA raw datasets of the skin and dermal papilla cells of secondary hair follicle (SHF-DPCs) samples of hair follicle (HF) anagen and telogen of Albas cashmere goats and identified a set of significant differentially expressed genes (DEGs). To explore potential associations between gene sets and SHF growth features and to identify candidate genes, we detected functional enrichment and constructed protein-protein interaction (PPI) networks...
March 25, 2020: International Journal of Molecular Sciences
Lionello Bossi, Nara Figueroa-Bossi, Philippe Bouloc, Marc Boudvillain
The largest and best studied group of regulatory small RNAs (sRNAs) in bacteria act by modulating translation or turnover of messenger RNAs (mRNAs) through base-pairing interactions that typically take place near the 5' end of the mRNA. This allows the sRNA to bind the complementary target sequence while the remainder of the mRNA is still being made, creating conditions whereby the action of the sRNA can extend to transcriptional steps, most notably transcription termination. Increasing evidence corroborates the existence of a functional interplay between sRNAs and termination factor Rho...
March 23, 2020: Biochimica et Biophysica Acta. Gene Regulatory Mechanisms
Jia Tian, Shao-Hua Zhang, Ke Ma, Bao-Yun Zhao, Bei Yan, Cheng-Bin Pei, Yue Zhou, Hong-Hong Wang, Hong-Yan Wang, Liang-Hong Ma, Xin-Zong Zhang
Objective: To investigate the influence of the Rho/ROCK signaling pathway on the anti-cryodamage ability of human sperm and provide some theoretical evidence for the development of high-efficiency semen cryoprotectants. METHODS: We collected semen samples from 25 healthy males, each divided into a fresh, a normal cryopreservation control and an Rho-inhibition group. Before and after freezing, we detected sperm motility, viability, membrane integrity, morphology, DNA fragmentation index (DFI), acrosomal enzyme activity (AEA) and mitochondrial membrane potential (MMP) and determined the expressions of RhoA and ROCK proteins in the sperm by immunofluorescence staining...
April 2019: Zhonghua Nan Ke Xue, National Journal of Andrology
Kun Liu, Aixiang Wang, Longke Ran, Wanfeng Zhang, Song Jing, Yujing Wang, Xianqin Zhang, Geli Liu, Wang Sen, Fangzhou Song
Prostate cancer (PCa) metastasis is considered the leading cause of cancer death in males. Therapeutic strategies and diagnosis for stage-specific PCa have not been well understood. Rho guanine nucleotide exchange factor 38 (ARHGEF38) is related to tumor cell polarization and is frequently expressed in PCa. Microarray data of PCa were downloaded from GEO and TCGA databases. A total of 243 DEGs were screened, of which, 32 genes were upregulated. The results of enrichment analysis showed the participation of these DEGs in the tumor cell metastasis pathway...
June 2020: Genes & Diseases
Anna Szaflarska-Popławska, Bartosz Romańczuk, Monika Parzęcka
Introduction: It is still unknown whether faecal calprotectin elevation may be caused by active untreated coeliac disease (CD) itself or whether it indicates the coexistence of CD and another disease associated with gastrointestinal inflammation. Aim: To assess faecal calprotectin concentration (FCC) and its correlation with the clinical form and histopathological picture of the small intestine in children with CD. Material and methods: Fifty-five children with newly recognised CD (mean age: 9...
2020: Przegla̜d Gastroenterologiczny
Sara R Alcorn, Lindsey Sloan, Todd R McNutt, Susan F Stinson, Fariba Asrari, Victoria J Croog, Bethlehem Floreza, Arcelia Weaver, Jean L Wright
Purpose: Adjacent tissues-in-beam (TIB) may receive substantial incidental doses within standard tangent fields during hypofractioned whole breast irradiation (HF-WBI). To characterize the impact of dose to TIB, we analyzed dosimetric parameters of TIB and associated acute toxicity. Materials and Methods: Plans prescribed to 40.5 Gy/15 fractions from 4/2016-1/2018 were evaluated. Structures of interest were contoured: (1) TIB: all tissues encompassed by plan 30% isodose lines, (2) breast, (3) non-breast TIB (nTIB): TIB minus contoured breast...
May 2020: Reports of Practical Oncology and Radiotherapy
Xintao Yang, Feng Hu, Jessica Aijia Liu, Shan Yu, May Pui Lai Cheung, Xuelai Liu, Irene Oi-Lin Ng, Xin-Yuan Guan, Kelvin K W Wong, Rakesh Sharma, Hong Lok Lung, Yufei Jiao, Leo Tsz On Lee, Martin Cheung
A Rho GTPase-activating protein (RhoGAP), deleted in liver cancer 1 (DLC1), is known to function as a tumor suppressor in various cancer types; however, whether DLC1 is a tumor-suppressor gene or an oncogene in melanoma remains to be clarified. Here we revealed that high DLC1 expression was detected in most of the melanoma tissues where it was localized in both the nuclei and the cytoplasm. Functional studies unveiled that DLC1 was both required and sufficient for melanoma growth and metastasis. These tumorigenic events were mediated by nuclear-localized DLC1 in a RhoGAP-independent manner...
March 25, 2020: Oncogene
Jakob Siedlecki, Valerie Schmelter, Benedikt Schworm, Wolfgang J Mayer, Siegfried G Priglinger, Martin Dirisamer, Nikolaus Luft
PURPOSE: To analyse in depth the associations between objectively measured corneal higher-order aberrations (HOAs) and subjectively perceived visual quality after small incision lenticule extraction (SMILE) as quantified with the standardized and clinically validated quality of vision (QOV) questionnaire. METHODS: This cross-sectional study included patients after bilateral simultaneous SMILE for the treatment of myopia and/or myopic astigmatism with plano target refraction...
March 25, 2020: Acta Ophthalmologica
Gustavo A Ospina-Tascón, Diego F Bautista, Humberto J Madriñán, Juan D Valencia, William F Bermúdez, Edgardo Quiñones, Luis Eduardo Calderón-Tapia, Glenn Hernandez, Alejandro Bruhn, Daniel De Backer
BACKGROUND: Ventilation/perfusion inequalities impair gas exchange in acute respiratory distress syndrome (ARDS). Although increased dead-space ventilation (VD /VT ) has been described in ARDS, its mechanism is not clearly understood. We sought to evaluate the relationships between dynamic variations in VD /VT and extra-pulmonary microcirculatory blood flow detected at sublingual mucosa hypothesizing that an altered microcirculation, which is a generalized phenomenon during severe inflammatory conditions, could influence ventilation/perfusion mismatching manifested by increases in VD /VT fraction during early stages of ARDS...
March 24, 2020: Annals of Intensive Care
Outi Linnaranta, Clément Bourguignon, Olivia Crescenzi, Duncan Sibthorpe, Asli Buyukkurt, Howard Steiger, Kai-Florian Storch
BACKGROUND: Sleep problems are common in eating disorders (EDs). PURPOSE: We evaluated whether sleep-phasing regularity associates with the regularity of daily eating events. METHODS: ED patients (n = 29) completed hourly charts of mood and eating occasions for 2 weeks. Locomotor activity was recorded continuously by wrist actigraphy for a minimum of 10 days, and sleep was calculated based on periods of inactivity. We computed the center of daily inactivity (CenDI) as a measure of sleep phasing and consolidation of the daily inactivity (ConDI) as a measure of daily sleep rhythm strength...
March 25, 2020: Annals of Behavioral Medicine: a Publication of the Society of Behavioral Medicine
Kerim Öner, Ahmet Emin Okutan, Muhammet Salih Ayas, Ahmet Emre Paksoy, Ferdi Polat
Background: Postoperative pain is well known and usually disturbing complication of arthroscopic shoulder surgery. Inflammation plays an important role in the development and progression of postoperative pain. The aim of this study was to evaluate the predictability of postoperative pain through the correlation of neutrophil/lymphocyte ratio (NLR) with inflammation. In addition, the correlation of parameters such as operative time, tear size, age and gender with postoperative pain was evaluated...
April 2020: Asia-Pacific Journal of Sports Medicine, Arthroscopy, Rehabilitation and Technology
Yiru Wang, Jiayu Shi, Qian Zheng, Bing Shi, Zhonglin Jia
BACKGROUND: Genome-wide association studies (GWAS) have found more than 20 genes associated with a risk of non-syndromic cleft lip with or without cleft palate (NSCL/P). However, the interactions between these risk genes have been rarely reported. METHODS: Here we selected 47 Single Nucleotide Polymorphisms (SNP) from previous GWASs and tested for possible interactions among 302 NSCL/P case-parent trios from a western Han Chinese population to further explore the genetic etiology of NSCL/P...
2020: International Journal of Clinical and Experimental Pathology
Gustavo A Chiprés-Tinajero, Miguel A Núñez-Ochoa, Laura Medina-Ceja
Physiological behaviours such as the sleep-wake cycle and exploratory behaviours are important parameters in intact and sham-operated animals and are usually thought to be unaffected by experimental protocols in which neurosurgery is performed. However, there is insufficient evidence in the literature on the behavioural and cognitive effects observed after deep microelectrode implantation surgery in animal models of neurological diseases. Similarly, in studies that utilize animal models of neurological diseases, the impact of surgery on the pathological phenomena being studied is often minimized...
2020: Behavioural Neurology
César F Cayo-Rojas, Leidy A Begazo-Jiménez, Luighy B Romero-Solórzano, Miriam K Nicho-Valladares, Andrea Gaviria-Martínez, Luis A Cervantes-Ganoza
Objective: To determine the relationship between the height of the periapical lesions adjacent to the maxillary sinus and the thickness of the Schneider membrane evaluated with cone-beam tomography. Materials and Methods . The universe was made up of 2432 tomography scans and a sample of 976, by systematic random sampling, and took into account those that presented any of the variables and/or both. For the relationship analysis, the sample was distributed according to sex, maxillary side, and age; it was formed between 18 and 86 years, in age groups of 18-36 years, 37-48 years, 49-59 years, and 60-86 years...
2020: International Journal of Dentistry
Jan Wilke, Heloise Debelle, Sarah Tenberg, Andrew Dilley, Constantinos Maganaris
Experiments in cadavers have demonstrated significant mechanical interactions between constituents of myofascial chains. However, evidence for such force transmission effects is scarce under in vivo conditions. The purpose of this trial was to examine the impact of ankle motion on soft tissue displacement of the dorsal thigh. Eleven healthy active individuals (26.8 ± 4.3 years, six males), in prone position and with the knee extended, underwent passive calf stretches (ankle dorsal extension) imposed by an isokinetic dynamometer...
2020: Frontiers in Physiology
Mirthe J Mebius, Catherina M Bilardo, Martin C J Kneyber, Marco Modestini, Tjark Ebels, Rolf M F Berger, Arend F Bos, Elisabeth M W Kooi
BACKGROUND: The exact onset of brain injury in infants with congenital heart disease (CHD) is unknown. Our aim was, therefore, to assess the association between prenatal Doppler flow patterns, postnatal cerebral oxygenation and short-term neurological outcome. METHODS: Prenatally, we measured pulsatility indices of the middle cerebral (MCA-PI) and umbilical artery (UA-PI) and calculated cerebroplacental ratio (CPR). After birth, cerebral oxygen saturation (rcSO2) and fractional tissue oxygen extraction (FTOE) were assessed during the first 3 days after birth, and during and for 24 hours after every surgical procedure within the first 3 months after birth...
2020: PloS One
Seungwon Jung, Jihoon Moon, Sungwoo Park, Seungmin Rho, Sung Wook Baik, Eenjun Hwang
For efficient and effective energy management, accurate energy consumption forecasting is required in energy management systems (EMSs). Recently, several artificial intelligence-based techniques have been proposed for accurate electric load forecasting; moreover, perfect energy consumption data are critical for the prediction. However, owing to diverse reasons, such as device malfunctions and signal transmission errors, missing data are frequently observed in the actual data. Previously, many imputation methods have been proposed to compensate for missing values; however, these methods have achieved limited success in imputing electric energy consumption data because the period of data missing is long and the dependency on historical data is high...
March 23, 2020: Sensors
Jinhyung Rho, Chang-Seob Seo, Hee-Seon Park, Hye-Yun Jeong, Og-Sung Moon, Young-Won Seo, Hwa-Young Son, Young-Suk Won, Hyo-Jung Kwun
ETHNOPHARMACOLOGICAL RELEVANCE: Asteris Radix et Rhizoma (AR) refers to the roots and rhizomes of Aster tataricus L., which is widely distributed throughout East Asia. AR has been consumed as a traditional medicine in Korea, Japan and China for the treatment of urologic symptoms. To date, however, the therapeutic effect of AR on benign prostatic hyperplasia (BPH) has not been investigated. AIM OF THE STUDY: The present study evaluated the therapeutic effects of AR on a testosterone-induced BPH rats...
March 21, 2020: Journal of Ethnopharmacology
Daniel M Muema, Ngomu A Akilimali, Okechukwu C Ndumnego, Sipho S Rasehlo, Raveshni Durgiah, Doty B A Ojwach, Nasreen Ismail, Mary Dong, Amber Moodley, Krista L Dong, Zaza M Ndhlovu, Jenniffer M Mabuka, Bruce D Walker, Jaclyn K Mann, Thumbi Ndung'u
INTRODUCTION: Immunological damage in acute HIV infection (AHI) may predispose to detrimental clinical sequela. However, studies on the earliest HIV-induced immunological changes are limited, particularly in sub-Saharan Africa. We assessed the plasma cytokines kinetics, and their associations with virological and immunological parameters, in a well-characterized AHI cohort where participants were diagnosed before peak viremia. METHODS: Blood cytokine levels were measured using Luminex and ELISA assays pre-infection, during the hyperacute infection phase (before or at peak viremia, 1-11 days after the first detection of viremia), after peak viremia (24-32 days), and during the early chronic phase (77-263 days)...
March 25, 2020: BMC Medicine
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"