Roman Goff, Morgan Smith, Sabrina Islam, Sue Sisley, Jonathan Ferguson, Scott Kuzdzal, Sunil Badal, Arun Babu Kumar, Uma Sreenivasan, Kevin A Schug
A method for clinical potency determination of psilocybin and psilocin in hallucinogenic mushroom species Psilocybe cubensis was developed using liquid chromatography with tandem mass spectrometry (LC-MS/MS). Five strains of dried, intact mushrooms were obtained and analyzed: Blue Meanie, Creeper, B-Plus, Texas Yellow, and Thai Cubensis. An extraction protocol was developed; this included an evaluation of sample milling technique, extraction solvents, and recovery/stability. Reversed phase chromatography on fused-core particle phases was developed for the determination of the two analytes using internal standard calibration with deuterated isotopologues of each analyte...
February 1, 2024: Analytica Chimica Acta