Damien Jacot, Garance Sarton-Lohéac, Alix T Coste, Claire Bertelli, Gilbert Greub, Guy Prod'hom, Antony Croxatto
OBJECTIVES: New automated modules are required to provide fully automated solutions in diagnostic microbiology laboratories. We evaluated the performance of a Becton Dickinson Kiestra™ IdentifA/SusceptA prototype for MALDI-TOF identification (ID) and PhoenixTM antibiotic susceptibility testing (AST). METHODS: The performance of the IdentifA/SusceptA coupled prototype was compared to manual processing for MALDI-TOF ID on 1302 clinical microbial isolates or ATCC strains and for PhoenixTM M50 AST on 484 strains, representing 61 species...
October 5, 2020: Clinical Microbiology and Infection
Christopher W Farnsworth, Meghan A Wallace, Albert Liu, Ann M Gronowski, Carey-Ann D Burnham, Melanie L Yarbrough
BACKGROUND: Every clinical specimen is potentially infectious, but data regarding risk for contamination of the laboratory environment during routine testing are scarce. We assessed contamination during routine sample analysis in automated clinical chemistry and microbiology laboratories. METHODS: A fluorescent marker was applied to specimen container exteriors to assess the impact of gross contamination. Nonpathogenic MS2 virus was added to remnant blood, urine, and ESwab matrices as a biomarker of cross-contamination...
September 1, 2020: Clinical Chemistry
James W Snyder, Gina K Thomson, Stacy Heckman, Kira Jamros, Sameh AbdelGhani, Kenneth S Thomson
OBJECTIVE: The current BD KiestraTM TLA automates specimen inoculation, incubation, and digital visualization of cultures prior to initiation of manual or semi-automated identification (ID) and antimicrobial susceptibility testing (AST). The current study aimed to compare performance, in a clinical setting, of a fully automated research-use only prototype, BD KiestraTM IdentifA/SusceptA (automated system), to our current BD KiestraTM , TLA which utilizes manual or semi-automated IDs and ASTs (current system) METHODS: Clinical samples yielding significant growth after processing by the BD KiestraTM TLA were tested in parallel for ID and AST by both systems...
July 25, 2020: Clinical Microbiology and Infection
Lisanne Kiestra, Iris A C de Vries, Bob C Mulder
This study aimed to examine the amount of lifestyle counseling that Dutch general practitioners (GPs) generally provide to their patients, as well as the behavioral determinants of their lifestyle counseling practices. Lifestyle counseling was defined and operationalized through the 5As model (i.e. Assess, Advise, Agree, Assist and Arrange), while determinants were based on an adapted version of the theory of planned behavior. A cross-sectional study was conducted among a sample of 198 GPs, using an online survey questionnaire for collecting data...
2020: PloS One
Pinli Yue, Menglan Zhou, Lintao Zhang, Qiwen Yang, Hongmei Song, Zhipeng Xu, Ge Zhang, Xiuli Xie, Yingchun Xu
Background: The aim of this study was to evaluate the clinical performance of the BD Kiestra InoqulA automated specimen processing system with commonly encountered clinical microbiology specimens. Materials and Methods: Four types of clinical specimens (sputum, urine, normally sterile body fluids, and feces) were inoculated onto relevant agar plates using a manual method and the BD Kiestra automated system. The number of isolated pathogen species, number of isolated single colonies and uniformity of plate streaking were calculated and compared between two methods...
2020: Infection and Drug Resistance
Cheng Wan Rong Carmen, Ong Chiou Horng, Chan Su Gin Douglas
OBJECTIVE: The objective of this study is to determine if using alternative streaking patterns on the BD Kiestra™ InoqulA™ can impact colony isolation and improve turnaround time (TAT) of methicillin-resistant Staphylococcus aureus (MRSA) and carbapenem-resistant Enterobacterales (CRE) screening samples. METHOD: A total of 1571 positive MRSA screening samples were studied of which 755 screening plates were streaked by the standard pattern (4-Quadrant uniform S200) and 816 plates were streaked by an alternative pattern (Zigzag 3...
January 11, 2020: Clinical Microbiology and Infection
M Desjardins, N Sant, M Miron-Celis, J Gosal, Marie-France Jémus, E Jémus-Gonzalez
We determined the impact of reducing incubation times for urine cultures incubated in BD Kiestra ReadA Compact incubators. Urine samples (n = 348) were inoculated to solid media, incubated in the ReadA Compacts and processed at 12, 15, and 18 h. Colony size and identification by MALDI-TOF, growth, semi-quantitation, Vitek and disk diffusion susceptibilities from cultures incubated at 12 and 15 h were compared to 18 h. There was no impact on MALDI-TOF performed after 12 and 15 versus 18 h of incubation. Interpretation for 99% of urine cultures was identical at 15 and 18 h...
October 5, 2019: Diagnostic Microbiology and Infectious Disease
Richard B Thomson, Erin McElvania
The first clinical microbiology laboratory in the United States adopted total automation for bacteriology processing in 2014. Since then, others have followed with installation of either the BD Kiestra TLA or the Copan WASPLab. This article discusses commercially available automated systems in the United States; why automation is needed; and quality improvements, efficiency, and cost savings associated with automation. After learning how these systems are used, gains and losses experienced, and how one can afford the most expensive equipment ever purchased for clinical microbiology laboratories, the question is, how can one afford not to purchase one of these microbiology automation systems?...
September 2019: Clinics in Laboratory Medicine
Melanie L Yarbrough, William Lainhart, Allison R McMullen, Neil W Anderson, Carey-Ann D Burnham
Total laboratory automation (TLA) has the potential to reduce specimen processing time, improve standardization of cultures, and decrease turnaround time (TAT). The objective of this study was to perform a detailed interrogation of the impact of TLA implementation in all aspects of the workflow for routine culture of urine specimens. Using a detailed motion capture study, the time required for major steps of processing and result reporting were prospectively assessed for urine samples prior to (n = 215) and after (n = 203) implementation of the BD Kiestra TLA system...
December 2018: European Journal of Clinical Microbiology & Infectious Diseases
Giuseppe Vittorio De Socio, Francesco Di Donato, Riccardo Paggi, Chiara Gabrielli, Alessandra Belati, Giuseppe Rizza, Martina Savoia, Antonella Repetto, Elio Cenci, Antonella Mencacci
The impact on time to results (TTR) and clinical decisions was evaluated for mono-microbial positive blood cultures (BC) processed using the BD Kiestra Work Cell Automation (WCA) system. Positive BC were processed by the WCA system by full-automatic subculture on solid media and digital imaging after 8 h of incubation (8-h method) followed by identification (ID) and antimicrobial susceptibility testing (AST). To evaluate the accuracy of the 8-h method, ID and AST from 8-h and overnight incubated colonies were compared for the same organisms...
September 14, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Irene Burckhardt, Susanne Horner, Florian Burckhardt, Stefan Zimmermann
In 2016, the workflow for MRSA detection in nasal swabs was changed from a classic-manual workflow to an automated workflow using total lab automation (TLA; BD Kiestra). This change entailed a reduction of the incubation time from 2 days to 20 h and reading of plates on weekdays and weekends instead of weekdays only. The workflow alteration did not include the introduction of 24/7. We wanted to follow up on the consequences for the times to report (TTR). We compared the TTR of all nasal swabs, which were sent for MRSA detection from June until August in 2015 (workflow-classic-manual) and in 2016 (workflow-automated)...
September 2018: European Journal of Clinical Microbiology & Infectious Diseases
Sabrina Klein, Dennis Nurjadi, Susanne Horner, Klaus Heeg, Stefan Zimmermann, Irene Burckhardt
While total laboratory automation (TLA) is well established in laboratory medicine, only a few microbiological laboratories are using TLA systems. Especially in terms of speed and accuracy, working with TLA is expected to be superior to conventional microbiology. We compared in total 35,564 microbiological urine cultures with and without incubation and processing with BD Kiestra TLA for a 6-month period each retrospectively. Sixteen thousand three hundred thirty-eight urine samples were analyzed in the pre-TLA period and 19,226 with TLA...
July 2018: European Journal of Clinical Microbiology & Infectious Diseases
Antony Croxatto, Raphaël Marcelpoil, Cédrick Orny, Didier Morel, Guy Prod'hom, Gilbert Greub
BACKGROUND: Automation in microbiology laboratories impacts management, workflow, productivity and quality. Further improvements will be driven by the development of intelligent image analysis allowing automated detection of microbial growth, release of sterile samples, identification and quantification of bacterial colonies and reading of AST disk diffusion assays. We investigated the potential benefit of intelligent imaging analysis by developing algorithms allowing automated detection, semi-quantification and identification of bacterial colonies...
December 2017: Biomedical Journal
Talent Theparee, Sanchita Das, Richard B Thomson
Technological advances have changed the practice of clinical microbiology. We implemented Bruker matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and BD Kiestra total laboratory automation (TLA) 4 and 3 years ago, respectively. To assess the impact of these new technologies, we compared turnaround times (TATs) for positive and negative urine cultures before and after implementation. In comparison I, TATs for 61,157 urine cultures were extracted for two periods corresponding to pre-TLA and post-TLA, both using MALDI-TOF MS for organism identification...
January 2018: Journal of Clinical Microbiology
C S Heather, M Maley
Early detection of resistance in sepsis due to Gram-negative organisms may lead to improved outcomes by reducing the time to effective antibiotic therapy. Traditional methods of resistance detection require incubation times of 18 to 48 h to detect resistance. We have utilised automated specimen processing, digital imaging and zone size measurements in conjunction with direct disc susceptibility testing to develop a method for the rapid screening of Gram-negative blood culture isolates for resistance. Positive clinical blood cultures with Gram-negative organisms were prospectively identified and additional resistant mock specimens were prepared...
January 2018: European Journal of Clinical Microbiology & Infectious Diseases
Inga Fröding, Martin Vondracek, Christian G Giske
Objectives: The need for rapid antibiotic susceptibility testing increases with escalating levels of antimicrobial resistance in Enterobacteriaceae. Our objective was to evaluate the accuracy of reading EUCAST disc diffusion, ROSCO ESBL and carbapenemase detection kits and the Mast Carbapenemase Activity Test (CAT-ID) disc, after 6 h of incubation. Methods: We used a collection of 128 isolates of Escherichia coli and Klebsiella pneumoniae with a wide variety of resistance mechanisms...
April 1, 2017: Journal of Antimicrobial Chemotherapy
Maryza Graham, Leanne Tilson, Richard Streitberg, John Hamblin, Tony M Korman
We compared the results of 505 urine specimens prospectively processed by both conventional manual processing (MP) with 16-24h incubation to BD Kiestra™ Total Laboratory Automation (TLA) system with a shortened incubation of 14h: 97% of culture results were clinically concordant. TLA processing was associated with improved standardization of time of first culture reading and total incubation time.
September 2016: Diagnostic Microbiology and Infectious Disease
A Croxatto, G Prod'hom, F Faverjon, Y Rochais, G Greub
Automation was introduced many years ago in several diagnostic disciplines such as chemistry, haematology and molecular biology. The first laboratory automation system for clinical bacteriology was released in 2006, and it rapidly proved its value by increasing productivity, allowing a continuous increase in sample volumes despite limited budgets and personnel shortages. Today, two major manufacturers, BD Kiestra and Copan, are commercializing partial or complete laboratory automation systems for bacteriology...
March 2016: Clinical Microbiology and Infection
Jesper Iversen, Gitta Stendal, Cecilie M Gerdes, Christian H Meyer, Christian Østergaard Andersen, Niels Frimodt-Møller
This study evaluated the quantitative results from and quality of the inoculation patterns of urine specimens produced by two automated instruments, the Copan WASP and the BD InoqulA. Five hundred twenty-six urine samples submitted in 10-ml canisters containing boric acid were processed within 30 min on an InoqulA instrument plating 10 μl of specimen, and on two WASP instruments, one plating 1 μl of specimen (WASP-1), and the second plating 10 μl of WASP (WASP-10). All samples were incubated, analyzed, and digitally imaged using the BD Kiestra total lab automation system...
February 2016: Journal of Clinical Microbiology
Michael Hombach, Florian P Maurer, Tamara Pfiffner, Erik C Böttger, Reinhard Furrer
Parameters like zone reading, inoculum density, and plate streaking influence the precision and accuracy of disk diffusion antibiotic susceptibility testing (AST). While improved reading precision has been demonstrated using automated imaging systems, standardization of the inoculum and of plate streaking have not been systematically investigated yet. This study analyzed whether photometrically controlled inoculum preparation and/or automated inoculation could further improve the standardization of disk diffusion...
December 2015: Journal of Clinical Microbiology
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