ovarian stimulation

Qian Peng, Xiang Cao, Jing Wang, Lin Wang, Jun Xu, Xiaowei Ji, Suying Liu, Jin Zhu, Xi Dong
BACKGROUND: Previous studies have demonstrated that progestin-primed ovarian stimulation (PPOS) protocol was a feasible and efficient method in in vitro fertilization (IVF) cycle. However, its application in women with advanced age has not been determined yet. The purpose of this study was to investigate its efficacy in women aged ≥40 years old. METHODS: This retrospective cohort study included patients with ages of ≥40 years old at the time of ovarian stimulation...
November 9, 2019: Reproductive Biology and Endocrinology: RB&E
Yanjie Chen, Shupei Zhang, Hong Dai, Zhensheng Hong, Yanyu Lin
A flexible two-step photoelectrochemical (PEC)-colorimetric immunoassay was proposed for ultrasensitive detection of lipolysis stimulated lipoprotein receptor (LSR) which is found to be closely related to ovarian cancer (OC). In this paper, the Cu nanoclusters (CuNCs) enhanced multiple mixed TiO2 mesocrystals junction (MMMJ) was fabricated via effective combination of multiple different phases TiO2 mesocrystals (Anatase and Rutile) layers and used as a sensing platform. The strong interaction between different phases layers caused multiple amplification of signal and introduction of Cu NCs further improve PEC properties and catalytic activity to hydrogen peroxide (H2 O2 ) what can catalyze lecuo-methylene blue (lecuo-MB) from colorless to blue...
October 26, 2019: Biosensors & Bioelectronics
Emily Garnett, Janet Bruno-Gaston, Jing Cao, Paul Zarutskie, Sridevi Devaraj
Successful outcomes of in vitro fertilization (IVF) are dependent in part on successful oocyte maturation and retrieval during a controlled ovarian stimulation process, which is guided by serial ultrasound and estradiol measurements. Yet, laboratory analysis of estradiol poses challenges due to the need for accuracy and specificity across concentrations that span multiple orders of magnitude. The Endocrine Society released a 2013 position statement that called for improvements in methods to analyze estradiol, and while some progress has been made in standardization and assay specificity, further work is needed to meet the needs of patients in both the IVF setting and in other clinical contexts...
November 6, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
Satoshi Imamura, Sung-Pyo Hur, Yuki Takeuchi, Muhammad Badruzzaman, Angka Mahardini, Dinda Rizky, Akihiro Takemura
The sapphire devil (Chrysiptera cyanea) is a tropical damselfish that undergoes active reproduction under long-day conditions. To elucidate the physiological regulation of the brain-pituitary-gonadal axis in female sapphire devil, we cloned and characterized the genes of two kisspeptins (kiss1 and kiss2), three gonadotropin-releasing hormones (gnrh1, gnrh2, gnrh3), and the β-subunit of two gonadotropins (fshβ and lhβ) and investigated the gene expression changes during ovarian development. Quantitative polymerase chain reaction analyses in various brain parts revealed high expression levels of kiss1, kiss2, and gnrh2 in the diencephalon; gnrh2 and gnrh3 in the telencephalon; and fshβ and lhβ in the pituitary...
November 8, 2019: Fish Physiology and Biochemistry
Maëliss Peigné, Emmanuel Devouche, Xavier Ferraretto, Solenne Gricourt, Dominique Luton, Catherine Patrat, Sylvie Epelboin
OBJECTIVE: To study which endometrial preparation allows a better ongoing pregnancy rates (OPR) and live birth rate (LBR) after frozen-thawed embryo transfer (FET) between mild gonadotropin ovarian stimulation (OS) and artificial cycles (AC). STUDY DESIGN: Retrospective follow-up study including all FET performed in one fertility center from 2013 to 2016. In the OS group, gonadotropins were followed by r-hCG triggering. Vaginal micronized progesterone (200 mg/day) was given systematically...
October 31, 2019: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Maciej Brązert, Wiesława Kranc, Piotr Celichowski, Katarzyna Ożegowska, Joanna Budna-Tukan, Michal Jeseta, Leszek Pawelczyk, Małgorzata Bruska, Maciej Zabel, Michał Nowicki, Bartosz Kempisty
Under physiological conditions, human ovarian granulosa cells (GCs), are responsible for a number of processes associated with folliculogenesis and oogenesis. The primary functions of GCs in the individual phases of follicle growth are: Hormone production in response to follicle stimulating hormone (FSH), induction of ovarian follicle atresia through specific molecular markers and production of nexus cellular connections for communication with the oocyte. In recent years, interest in obtaining stem cells from particular tissues, including the ovary, has increased...
September 26, 2019: Molecular Medicine Reports
Danilo Cimadomo, Ramona Carmelo, Elvira Immacolata Parrotta, Stefania Scalise, Gianluca Santamaria, Erminia Alviggi, Maria Teresa De Angelis, Gianmarco Sarro, Alberto Vaiarelli, Roberta Venturella, Laura Rienzi, Fulvio Zullo, Filippo Maria Ubaldi, Giovanni Cuda
PURPOSE: To detect putative differences in the miRNomic profile of follicular fluids collected after follicular-phase-stimulation (FPS-FFs) and paired luteal-phase-stimulation (LPS-FFs) in the same ovarian cycles (DuoStim). METHODS: Exploratory study at a private IVF center and University involving FPS-FFs and paired-LPS-FFs collected from 15 reduced ovarian reserve and advanced maternal age women undergoing DuoStim (n = 30 paired samples). The samples were combined in 6 paired pools (5 samples each) and balanced according to maternal age and number of cumulus-oocyte-complexes...
November 7, 2019: Journal of Assisted Reproduction and Genetics
Martina Belli, Christian Secchi, Dwayne Stupack, Shunichi Shimasaki
Adult granulosa cell tumor (aGCT) is a rare type of ovarian cancer characterized by estrogen excess. Interestingly, only the single somatic mutation FOXL2 C134W was found across virtually all aGCTs. We previously reported that FOXL2C134W stimulates CYP19 transcription synergistically with SMAD3, leading to elevated estradiol synthesis in a human granulosa cell line (HGrC1). This finding suggested a key role for FOXL2C134W in causing the typical estrogen overload in patients with aGCTs. We have now investigated the effect of FOXO1, a tumor suppressor, on CYP19 activation by FOXL2C134W in the presence of SMAD3...
November 1, 2019: Journal of the Endocrine Society
Aulona Gaba, Steffen Hörath, Marlene Hager, Rodrig Marculescu, Johannes Ott
PURPOSE: Recent studies reported that in polycystic ovary syndrome (PCOS) patients, other stimulation agents are superior to the popular first-line regimen, clomiphene citrate (CC) for ovarian stimulation. Nonetheless, CC is still widely used since it is not clear which patients will not respond to it. Furthermore, the prognostic value of endometrium thickness at midcycle is controversial. We aimed to find factors predicting the response to CC and the prognostic value of endometrial thickness at midcycle...
November 6, 2019: Archives of Gynecology and Obstetrics
Rosa Linares, Gabriela Rosas, Elizabeth Vieyra, Deyra A Ramírez, Daniel R Velázquez, Julieta A Espinoza, Carolina Morán, Roberto Domínguez, Leticia Morales-Ledesma
In rats with polycystic ovarian syndrome (PCOS) induced by estradiol valerate (EV) injection, sectioning of the vagus nerve in the juvenile stage restores ovulatory function, suggesting that the vagus nerve stimulates the onset and development of PCOS. We analyzed whether in adult rats, the role played by the vagus nerve in PCOS development is associated with the nerve's regulation of noradrenergic activity in the celiac superior mesenteric ganglion (CSMG). Ten-day-old rats were injected with corn oil [vehicle (Vh)] or EV (2 mg)...
2019: Frontiers in Physiology
Andressa Gonsioroski, Daryl D Meling, Liying Gao, Michael J Plewa, Jodi A Flaws
The reactions between disinfectants and organic matter or inorganic matter in source water generates disinfection by-products (DBPs) such as iodoacetic acid (IAA). DBPs are associated with health effects such as bladder cancer and adverse reproductive outcomes, but the effects of IAA on the ovary are not well known. This study determined whether IAA exposure affects ovarian follicle growth, steroidogenesis, and expression of apoptotic factors, cell cycle regulators, estrogen receptors, and steroidogenic factors in vitro...
November 3, 2019: Reproductive Toxicology
Edson Borges, Maria Gabriela Ferreira Mulato, Amanda Souza, Assumpto Iaconelli, Murilo Vieira, Daniela Paes
OBJECTIVE: To identify potential microRNA (miRNA) biomarkers of poor, normal and hyperresponse to controlled ovarian stimulation (COS). METHODS: In the present study, we assessed 40 serum samples from patients undergoing COS. We used ten samples to standardize miRNAs detection in the serum. The remaining 30 samples were split into three groups depending on the patient's response to COS: poor response (PR group, n=10), normal response (NR group, n=10), and hyperresponse (HR group, n=10)...
November 6, 2019: JBRA Assisted Reproduction
Meryem Kuru Pekcan, Esma Sarıkaya, Aytekin Tokmak, Hasan Ali İnal, Nafiye Yılmaz
BACKGROUND: The underlying cause of seasonal infertility in humans is unclear, but is likely to be -multifactorial. OBJECTIVE: The aim of our study was to compare the pregnancy rates among infertile women who underwent induced ovulation and intrauterine insemination (IUI) with the season in which the fertility treatment was performed. DESIGN AND SETTING: This retrospective cohort study was conducted on 466 patients who were treated in the reproductive endocrinology and infertility outpatient clinic of a tertiary-level women's healthcare and maternity hospital...
2019: São Paulo Medical Journal
Ya Liu, Xiaocui He, Yuli Chen, Dan Cao
Ovarian cancer (OC) is the most lethal gynecologic malignancy and long non-coding RNAs (lncRNAs) have been acknowledged as important regulators in human OC. This study aimed to investigate the function and underlying mechanisms of LINC00504 in OC. The expression levels of LINC00504 in human OC tissues and cell lines were investigated by qRT-PCR analysis. The OC cell proliferation, and apoptosis were evaluated by MTT assay, colony-formation assay, Caspase-3 activity assay, and nucleosome ELISA assay, respectively...
November 5, 2019: Molecular and Cellular Biochemistry
Kenichiro Sakaguchi, Yojiro Yanagawa, Koji Yoshioka, Tomoko Suda, Seiji Katagiri, Masashi Nagano
BACKGROUND: The antral follicle count (AFC) in mammalian ovaries positively correlates with female fertility. To clarify the causes of differences in fertility between low and high AFC cows, we investigated follicular growth dynamics and hormone concentrations in plasma, follicular fluid, and in vitro growth (IVG) media at different stages of follicular growth. METHODS: Seven cows were divided into high AFC (n = 4, > 30 follicles) and low AFC (n = 3, < 30 follicles) groups based on the peak AFC detected by ultrasonography...
November 5, 2019: Reproductive Biology and Endocrinology: RB&E
Motohiro Kano, Jennifer Y Hsu, Hatice D Saatcioglu, Nicholas Nagykery, LiHua Zhang, Mary E Morris Sabatini, Patricia K Donahoe, David Pépin
Müllerian-inhibiting substance (MIS), also known as anti-Müllerian hormone, is thought to be a negative regulator of primordial follicle activation. We have previously reported that treatment with exogenous MIS can induce complete ovarian suppression within 5 weeks of treatment in mice. To investigate the kinetics of the return of folliculogenesis following the reversal of suppression, we treated animals with recombinant human MIS (rhMIS) protein for 40 days in adult female Nu/Nu mice and monitored the recovery of each follicle type over time...
November 1, 2019: Journal of the Endocrine Society
Vasantha Padmanabhan, Rodolfo C Cardoso
Follicle-stimulating hormone (FSH) is a glycoprotein hormone produced by gonadotropes in the anterior pituitary that plays a central role in controlling ovarian folliculogenesis and steroidogenesis in females. Moreover, recent studies strongly suggest that FSH exerts extragonadal actions, particularly regulating bone mass and adiposity. Despite its crucial role, the mechanisms regulating FSH secretion are not completely understood. It is evident that hypothalamic, ovarian, and pituitary factors are involved in the neuroendocrine, paracrine, and autocrine regulation of FSH production...
November 1, 2019: Molecular and Cellular Endocrinology
Xi Shen, Hui Long, Wenya Guo, Hongyuan Gao, Renfei Cai, Wei Jin, Zhiguang Yan, Shaozhen Zhang, Yun Wang, Qifeng Lyu, Li Wang, Yanping Kuang
Background: To investigate the optimal ovulation trigger-oocyte pickup (OPU) interval of a progestin-primed ovarian stimulation (PPOS) protocol. Method: Patients with normal ovarian reserve in their first PPOS OPU cycle were enrolled in this retrospective cohort study between July 2013 and April 2018. This retrospective cohort study included two parts. In part I, we studied the regression trend of mature oocyte rate, implantation rate, and live birth rate within the whole ovulation trigger-OPU interval of 7,258 patients...
2019: Frontiers in Endocrinology
Chen-Chi Duan, Cheng Li, Yi-Chen He, Jing-Jing Xu, Chao-Yi Shi, Hong-Tao Hu, Yun-Fei Su, Lei Chen, Ya-Jing Tan, Zhi-Wei Liu, Jian-Zhong Sheng, William D Fraser, Yan-Ting Wu, He-Feng Huang
Maternal supraphysiological estradiol (E2) environment during pregnancy leads to adverse perinatal outcomes. However, the influence of oocyte exposure to high E2 levels on perinatal outcomes remains unknown. Thus, a retrospective cohort study was conducted to explore the effect of high E2 level induced by controlled ovarian stimulation (COH) on further outcomes after frozen embryo transfer (FET). The study included all FET cycles (n = 10,581) between 2014 and 2017. All cycles were categorized into three groups according to the E2 level on the day of the human Chorionic Gonadotropin trigger...
November 4, 2019: Journal of Developmental Origins of Health and Disease
Qian-Qian Sha, Yu Jiang, Chao Yu, Yunlong Xiang, Xing-Xing Dai, Jun-Chao Jiang, Xiang-Hong Ou, Heng-Yu Fan
CxxC-finger protein 1 (CFP1)-mediated trimethylated histone H3 at lysine-4 (H3K4me3) during oocyte development enables the oocyte genome to establish the competence to generate a new organism. Nevertheless, it remains unclear to which extent this epigenetic modification forms an instructive component of ovarian follicle development. We investigated the ovarian functions using an oocyte-specific Cxxc1 knockout mouse model, in which the H3K4me3 accumulation is downregulated in oocytes of developing follicles...
November 1, 2019: Cellular and Molecular Life Sciences: CMLS
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"