Rachel S Perkins, Glenn Murray, Sarocha Suthon, Lindsey Davis, Nicholson B Perkins, Lily Fletcher, Amanda Bozzi, Saylor L Schreiber, Jianjian Lin, Steven Laxton, Rahul R Pillai, Alec J Wright, Gustavo A Miranda-Carboni, Susan A Krum
BACKGROUND: Treatment for osteosarcoma, a paediatric bone cancer with no therapeutic advances in over three decades, is limited by a lack of targeted therapies. Osteosarcoma frequently metastasises to the lungs, and only 20% of patients survive 5 years after the diagnosis of metastatic disease. We found that WNT5B is the most abundant WNT expressed in osteosarcoma tumours and its expression correlates with metastasis, histologic subtype and reduced survival. METHODS: Using tumor-spheroids to model cancer stem-like cells, we performed qPCR, immunoblotting, and immunofluorescence to monitor changes in gene and protein expression...
May 2024: Clinical and Translational Medicine