Hamid Solgi, Fereshteh Shahcheraghi, Negin Bolourchi, Ali Ahmadi
Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) has been increasingly reported and is now recognized as a public health concern. The aim of this study was to investigate the molecular epidemiology of CR-hvKp strains that were isolated from an Iranian hospital. A total of 74 non-duplicated carbapenem-resistant K. pneumoniae (CR-Kp) were collected from patients' clinical or surveillance cultures. Resistance/virulence genes were identified by PCR and sequencing. String test, capsular genotyping, conjugation assays, PCR-based replicon typing, pulsed field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and were performed...
September 17, 2020: Microbial Pathogenesis
Yeben Ma, Xuebin Xu, Yuan Gao, Zeqiang Zhan, Chenggang Xu, Xiaoyun Qu, Zhengquan Chen, Jie Bai, Ming Liao, Jianmin Zhang
In this study, 205 Salmonella enterica serovar Corvallis strains were obtained from humans and foods from Guangdong, Guangxi, and Shanghai in China from 2009 to 2017 to assess drug resistance and molecular epidemiology. These isolates displayed high rates of resistance to sulfisoxazole (94.15%) and tetracycline (77.56%). Surprisingly, the rate of resistance to ciprofloxacin reached 21.46%. Moreover, 63.9% of the strains displayed multidrug resistance. Detection of quinolone genes showed that 97.56% of the strains had single mutations (T57S) in parC...
September 8, 2020: International Journal of Food Microbiology
Priscylla C Vasconcelos, Elma L Leite, Wydemberg J Araújo, Núbia M V Silva, Mauro M S Saraiva, Lauro S Filho, Oliveiro C Freitas Neto, Patricia E N Givisiez, Celso J B Oliveira
OBJECTIVES: Considering that polymyxin is a drug of last resort in the treatment of humans infected by multidrug-resistant bacteria, the occurrence of plasmid-mediated colistin resistance mcr gene among Gram-negative bacteria in foods must be investigated. We present herein the draft genome sequence of a phenotypically colistin-resistant Escherichia coli carrying mcr-1 in chicken carcasses from a public market. METHODS: Total genomic DNA from the strain was sequenced by means of the Illumina MiSeq...
September 11, 2020: Journal of Global Antimicrobial Resistance
Jonathan Moffat, Gabhan Chalmers, Richard Reid-Smith, Michael R Mulvey, Agnes Agunos, Julie Calvert, Ashley Cormier, Nicole Ricker, J Scott Weese, Patrick Boerlin
The goal of this study was to determine the frequency of resistance to extended-spectrum cephalosporins (ESCs) in Escherichia coli and other Enterobacterales from turkeys in Canada and characterize the associated resistance determinants. Pooled fecal samples were collected in 77 turkey farms across British Columbia, Québec, and Ontario. Isolates were obtained with and without selective enrichment cultures and compared to isolates from diagnostic submissions of suspected colibacillosis cases in Ontario. Isolates were identified using MALDI-TOF and susceptibility to ESCs was assessed by disk diffusion...
2020: PloS One
Giancarlo Ripabelli, Michela Lucia Sammarco, Angelo Salzo, Massimiliano Scutellà, Valentina Felice, Manuela Tamburro
The emergence of novel resistant markers hampers the efficacy of beta-lactam antibiotics to treat infections caused by microrganisms carrying such resistances. This study investigated the antimicrobial susceptibility pattern, the carpapenem associated determinants and the molecular epidemiology of Klebsiella pneumoniae showing a New Delhi (NDM) metallo-β-lactamase phenotype, isolated from a patient admitted to intensive care unit of the main hospital for acute care of Molise region, central Italy. Antimicrobial susceptibility was assessed for nineteen antibiotics by disc diffusion and agar dilution methods...
September 11, 2020: Letters in Applied Microbiology
Ahmad Farajzadeh Sheikh, Maryam Moradi Bandbal, Morteza Saki
Owing to the scarce evidence about the multidrug-resistant (MDR) beta-lactamase-producing Shigella isolates in Iran, this study aimed to evaluate the occurrence of extended-spectrum beta-lactamases (ESBL) and AmpC β-lactamases in Shigella species collected in the southwest of Iran. This study was conducted on Shigella species isolated from stool samples of pediatric patients aged less than 15 years suffering from diarrhea. These isolates were identified by bacteriology tests, serotyping, and polymerase chain reaction (PCR)...
September 7, 2020: Molecular Biology Reports
Preeti Chaturvedi, Deepshi Chaurasia, Ashok Pandey, Pratima Gupta
Wastewater effluents released in surface water provides suitable nutrient rich environment for the growth and proliferation of antibiotic resistant bacteria (ARB) and genes (ARG). Consequently, bacterial resistance has highly evolved over the recent years and diversified that each antibiotic class is inhibited by a distinct mechanism. In the present study, the prevalence of Multidrug resistant (MDR), extended spectrum β-lactamases (ESBL) and plasmid mediated Amp-C producing strains was analyzed in 28 surface water samples collected near domestic effluent discharge sites in river Ganga located across 11 different geographical indices of Uttar Pradesh, India...
August 28, 2020: Environmental Pollution
Maqsud Hossain, Tahmina Tabassum, Aura Rahman, Arman Hossain, Tamanna Afroze, Abdul Mueed Ibne Momen, Abdus Sadique, Mrinmoy Sarker, Fariza Shams, Ahmed Ishtiaque, Abdul Khaleque, Munirul Alam, Anwar Huq, Gias U Ahsan, Rita R Colwell
Escherichia coli is a pathogen commonly encountered in clinical laboratories, and is capable of causing a variety of diseases, both within the intestinal tract (intestinal pathogenic strains) and outside (extraintestinal pathogenic E. coli, or ExPEC). It is associated with urinary tract infections (UTIs), one of the most common infectious diseases in the world. This report represents the first comparative analysis of the draft genome sequences of 11 uropathogenic E. coli (UPEC) strains isolated from two tertiary hospitals located in Dhaka and Sylhet, Bangladesh, and is focused on comparing their genomic characteristics to each other and to other available UPEC strains...
September 3, 2020: Scientific Reports
Abhiroop Banerjee, K Batabyal, A D Singh, S N Joardar, S Dey, D P Isore, T K Sar, T K Dutta, S Bandyopadhyay, I Samanta
Antimicrobial resistance is a global emergency which needs one health approach to address. The present study was conducted to detect the prevalence of beta-lactamase and biofilm-producing-Klebsiella strains in rectal swabs (n=624) collected from healthy dogs, cats, sheep and goats reared as companion or household animals in India. The dogs and cats were frequently exposed to third or fourth generation cephalosporins for therapy.The sheep and goats were occasionally exposed to antibiotics and had environmental exposure...
September 3, 2020: Letters in Applied Microbiology
Hiromi Nagaoka, Shinichiro Hirai, Hirotaka Morinushi, Shiro Mizumoto, Kana Suzuki, Hiroaki Shigemura, Naoto Takahashi, Fumie Suzuki, Mizuha Mochizuki, Michiko Asanuma, Takaharu Maehata, Aya Ogawa, Kai Ohkoshi, Tsuyoshi Sekizuka, Taisei Ishioka, Satowa Suzuki, Hirokazu Kimura, Makoto Kuroda, Motoi Suzuki, Koichi Murakami, Takashi Kanda
Hospital-acquired infections caused by extended-spectrum β-lactamase (ESBL)-producing Escherichia coli are a global problem. Healthy people can carry ESBL-producing E. coli in the intestines; thus, E. coli from healthy people can potentially cause hospital-acquired infections. Therefore, the transmission routes of ESBL-producing E. coli from healthy persons should be determined. A foodborne outbreak of human norovirus (HuNoV) GII occurred at a restaurant in Shizuoka, Japan, in 2018. E. coli O25:H4 was isolated from some of the HuNoV-infected customers...
September 1, 2020: Journal of Food Protection
Juanjuan Zhou, Junwen Yang, Fupin Hu, Kaijie Gao, Jiufeng Sun, Junmei Yang
Background: Ceftazidime/avibactam (CZA)-resistant carbapenem-resistant Klebsiella pneumoniae (CRKP) infections occur in adults worldwide but are rarely observed in neonates. We evaluated the activities of CZA against CRKP and described the clinical and molecular epidemiology of CZA-resistant CRKP in a NICU prior to CZA approval in China. Methods: A laboratory-based surveillance of CRKP was conducted from July 2017 to June 2018. Clinical data were initially reviewed...
2020: Infection and Drug Resistance
Yi Yang, Yalan Peng, Jingyi Jiang, Zaicheng Gong, Hao Zhu, Kun Wang, Qiaoni Zhou, Yu Tian, Aijian Qin, Zhangping Yang, Shaobin Shang
Antimicrobials are the most important therapy to bovine mastitis. Bacterial infection and antibiotic treatment of mastitis cycles frequently in dairy farms worldwide, giving rise to concerns about the emergence of multidrug-resistant (MDR) bacteria. In this study, we examined the microbial diversity and antibiotic resistance profiles of bacteria isolated from raw milk from dairy farms in Jiangsu and Shandong provinces, China. Raw milk samples were collected from 857 dairy cattle including 800 apparently healthy individuals and 57 cows with clinical mastitis (CM) and subjected to microbiological culture, antimicrobial susceptibility assay and detection of antibiotic-resistant genes by polymerase chain reaction (PCR) and sequencing...
August 11, 2020: Transboundary and Emerging Diseases
Sneha Radha, Malathi Murugesan, Priscilla Rupali
PURPOSE OF REVIEW: Recent attempts at mapping Typhoid epidemiology have revealed an enormous burden of disease in developing countries. Countries hitherto believed to have a low incidence, such as the African subcontinent, on accurate mapping were found to have a significant burden of disease. Drug resistance, because of rampant overuse of antibiotics, has driven selection pressure to extensively drug-resistant typhoid becoming a reality in the Indian subcontinent. With widespread travel, importation of this variety of typhoid to nonendemic countries is likely to lead to outbreaks in a nonimmune population...
August 6, 2020: Current Opinion in Infectious Diseases
Panliang Wang, Ziru Qiao, Xunan Li, Dong Wu, Bing Xie
The prevalence and dissemination of antibiotic resistance genes (ARGs) have been globally gained increasing concerns. However, the fate and spread of ARGs in food waste (FW) and its large-scale biotreatment systems are seldomly understood. Here, we investigated the initial and biologically treated FW in two major FW treatment systems of aerobic fermentation (AF) and anaerobic co-digestion (AcoD) processes. The total relative abundances of integrons and ARGs significantly increased from initial FW to treated FW...
August 6, 2020: Environment International
Ding-Qiang Chen, Tengyi Huang, Qun Wang, Caiying Bai, Ling Yang
Escherichia coli is one of the most diverse microbial species. Pathogenic E. coli is capable of causing various diseases in humans, including several types of diarrhea, urinary tract infections, sepsis, and meningitis. This study focused on the antibiotic susceptibility profile and genomic analysis of a clinical E. coli Guangzhou-Eco330 isolated from a hospitalized 8-year-old female patient suffered from pulmonary infection in 2017. Susceptibility to 15 antibiotics were determined using Vitek2™ Automated Susceptibility System and Etest strips and interpreted based on CLSI guidelines...
August 5, 2020: Microbial Pathogenesis
Liam J Reynolds, Laura Sala-Comorera, Niamh A Martin, Tristan M Nolan, Jayne H Stephens, Aurora Gitto, Gregory M P O'Hare, John J O'Sullivan, Wim G Meijer
Antibiotic resistance represents the greatest challenge to healthcare systems around the world. As antibiotic resistance genes (ARGs) are shed in faeces, many studies have focused on how wastewater effluent contributes to ARG pollution in rivers. However, small urban streams and bathing waters not impacted by treated wastewater have received little attention though they may be important reservoirs of ARGs. The main objective of this study was to assess the extent to which ARG and faecal pollution impact small urban streams and bathing waters and to determine if there is a relationship between these contaminants...
June 17, 2020: Science of the Total Environment
Isabel Carvalho, María Teresa Tejedor-Junco, Margarita González-Martín, Juan Alberto Corbera, Alejandro Suárez-Pérez, Vanessa Silva, Gilberto Igrejas, Carmen Torres, Patrícia Poeta
Antimicrobial resistance among isolates from wild animals is increasingly reported. Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae, and particularly Escherichia coli, have spread worldwide as one of the most common multidrug resistant organisms. The aim of this study was to determine the carriage rate of ESBL-producing E. coli isolates and genetic characteristics in wild vultures from Canary Islands. Fecal samples were collected from 22 apparently healthy free-ranging (wild) vulture chicks from Lanzarote and Fuerteventura (Canary Islands) during July 2019...
August 4, 2020: Environmental Microbiology Reports
Haiyan Qin, Yidan Guo, Yikun Li, Rui Zheng
Purpose: Salmonella enterica serovar Typhimurium infection is common in foodborne diseases, but its isolation from surgical incisions is rare. Our aim in this study was to trace the transmission source of a surgical incision infected with S . Typhimurium in a Yunnan Province hospital patient and elucidate the underlying molecular mechanisms of antibiotic resistance. Methods: Primers were designed to amplify the drug-resistance genes using polymerase chain reaction (PCR)...
2020: Infection and Drug Resistance
Chunyan Xu, Ziquan Lv, Yingbo Shen, Dejun Liu, Yulin Fu, Lan Zhou, Weiwen Liu, Kun Chen, Hailing Ye, Xi Xia, Junjie Xia, Yang Wang, Yuebin Ke, Jianzhong Shen
Cumulative research on resistomes and microbiomes from aquatic environments has revealed that both integrated freshwater and monoculture freshwater aquaculture systems can cause the development and dissemination of antibiotic resistance genes (ARGs) and associated mobile genetic elements (MGEs). However, few studies have examined differences in resistomes between the different aquaculture modes, and those that do have focused on antibiotic residues or individual resistance genes. In the current study, we collected 44 environmental samples from two monoculture freshwater aquaculture farms and four integrated farms (two duck and fish farms, two laying duck and fish farms) in Guangdong, China, in 2018...
July 30, 2020: Environment International
Ahmed M Soliman, Hazem Ramadan, Mustafa Sadek, Hirofumi Nariya, Toshi Shimamoto, Lari M Hiott, Jonathan G Frye, Charlene R Jackson, Tadashi Shimamoto
OBJECTIVE: To describe the first draft genome sequence of a multidrug-resistant Escherichia coli D: ST69 clinical isolate from Egypt carrying blaNDM-1 - and blaOXA-244 . MATERIAL AND METHODS: The strain was isolated in December 2014 from a wound pus swab of a male patient in the city of Kafr El-Sheikh, Egypt using MacConkey agar containing 2 μg/ml meropenem. The isolate was subjected to antimicrobial susceptibility testing, conjugation experiments and whole genome sequencing using Illumina MiSeq platform...
July 29, 2020: Journal of Global Antimicrobial Resistance
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