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JOURNAL ARTICLE
Alireza Mohammadzadeh, Hamid Naghizadeh, Ahmad Mosadegh, Akram Astani, Omid Pouresmaeil, Jalal Mardaneh
BACKGROUND: Urinary tract infection (UTI) is one of the common bacterial infections. Escherichia coli is the most common cause of UTI. In this research, the prevalence of several virulence factors and beta-lactam resistance genes was investigated. METHODS: One hundred E. coli isolates were collected from patients' specimens with UTI referred to Allame-Bohlol Gonabadi hospital. Polymerase chain reaction (PCR) was performed to identify five pathogenic genes ( fimH, aer, pap, hly, traT ) and three antibiotic resistance genes ( blaTEM, blaCTX, blaSHV )...
October 2023: Reports of Biochemistry & Molecular Biology
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JOURNAL ARTICLE
Aarti Pustam, Jayaraj Jayaraman, Adesh Ramsubhag
OBJECTIVES: Antibiotic-resistant Klebsiella pneumoniae is a human pathogen of major global concern due to its ability to cause multiple severe diseases that are often difficult to treat therapeutically. This study aimed to investigate the resistome of local clinical K. pneumoniae isolates. METHODS: Herein, we used a whole genome sequencing approach and bioinformatics tools to reconstruct the resistome of ten clinical K. pneumoniae isolates and one clinical isolate of the closely related Klebsiella quasipneumoniae obtained from patients from three major hospitals in Trinidad, West Indies...
April 10, 2024: Journal of Global Antimicrobial Resistance
#3
JOURNAL ARTICLE
Roxana Zamudio, Patrick Boerlin, Michael R Mulvey, Marisa Haenni, Racha Beyrouthy, Jean-Yves Madec, Stefan Schwarz, Ashley Cormier, Gabhan Chalmers, Richard Bonnet, George G Zhanel, Heike Kaspar, Alison E Mather
BACKGROUND: Extended-spectrum cephalosporins (ESCs) are third and fourth generation cephalosporin antimicrobials used in humans and animals to treat infections due to multidrug-resistant (MDR) bacteria. Resistance to ESCs (ESC-R) in Enterobacterales is predominantly due to the production of extended-spectrum β-lactamases (ESBLs) and plasmid-mediated AmpC β-lactamases (AmpCs). The dynamics of ESBLs and AmpCs are changing across countries and host species, the result of global transmission of ESC-R genes...
April 11, 2024: EBioMedicine
#4
JOURNAL ARTICLE
Solomon Olabiyi Olorunleke, Miranda Kirchner, Nicholas Duggett, Kim Stevens, Kennedy F Chah, John A Nwanta, Lucy A Brunton, Muna F Anjum
The emergence and spread of β-lactamase-producing Enterobacteriaceae poses a significant threat to public health, necessitating the rapid detection and investigation of the molecular epidemiology of these pathogens. We modified a multiplex real-time (RT)-PCR to concurrently detect β-lactamase genes (blaCTX-M, blaTEM, and blaSHV) and Enterobacteriaceae 16S ribosomal RNA. qPCR probes and primers were validated using control isolates, and the sensitivity and specificity assessed. The optimised multiplex qPCR was used to screen 220 non-clinical Enterobacteriaceae from food animals and in-contact humans in Southeast Nigeria selected on cefotaxime-supplemented agar plates...
2024: PloS One
#5
(no author information available yet)
[This corrects the article DOI: 10.1371/journal.pone.0264439.].
2024: PloS One
#6
JOURNAL ARTICLE
Elizabeth A Leighton, Chelsea N Gale, En Huang, Xu Yang, Erin L DiCaprio, Xinhui Li
Fresh vegetables can harbor antibiotic-resistant bacteria, including extended-spectrum β-lactamase (ESBL)-producing Enterobacterales. Enterobacter hormaechei is a bacterium belonging to the Enterobacterales order and the most commonly identified nosocomial pathogen of Enterobacter cloacae complex. The purpose of this study was to characterize a multi-drug resistant ESBL-producing E. hormaechei strain isolated from a sample of mixed sprouts. Vegetable samples were pre-enriched in buffered peptone water, followed by enrichment in Enterobacteria Enrichment Broth, and isolation on Chromagar™ ESBL plates...
April 9, 2024: Current Microbiology
#7
JOURNAL ARTICLE
Francisco González-Espinosa, Vincenzo Di Pilato, Francisco Magariños, Jose Di Conza, Gian Maria Rossolini, Gabriel Gutkind, Marcela Radice, Daniela Cejas
OBJECTIVE: to describe at genomic level nine carbapenemase-producing Klebsiella pneumoniae ST307 (Kp-ST307) clinical isolates recovered in Buenos Aires during 2017-2021, investigating their resistome, virulome and phylogeny. METHODS: Antimicrobial susceptibility was determined according to CLSI. Genomic DNA was sequenced by Illumina MiSeq and analyzed using SPAdes, PROKKA and Kleborate. Phylogeny of 355 randomly selected Kp-ST307 genomes and those from nine local isolates was inferred by a maximum-likelihood approach...
April 5, 2024: Journal of Global Antimicrobial Resistance
#8
JOURNAL ARTICLE
Francis Kwame Morgan Tetteh, Anthony Ablordey, Noah Obeng-Nkrumah, Japheth Awuletey Opintan
INTRODUCTION: Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae are pathogens of significant public health interest for which new antibiotics are urgently needed. AIM: To determine the prevalence of ESBLs in E. coli and K. pneumoniae isolates from patients attending the Tamale Teaching Hospital (TTH) in Ghana. METHODOLOGY: The study was a cross-sectional study involving convenience sampling of E. coli and K...
2024: PloS One
#9
JOURNAL ARTICLE
Getahun E Agga, Lisa M Durso, Karamat R Sistani
Given the high cost and non-renewability of mineral-based fertilizers, there is increasing interest in the innovative use of manure-based materials, such as poultry litter (PL). However, manure-based fertilizers add both nutrients and microbes to the soil, including antibiotic-resistant Escherichia coli (AREc). PL soil amendment impact on AREc in corn fields was evaluated in a randomized field experiment (May-October 2017). Two winter cropping systems (fallow and cover crop) were assigned to whole plots, with three spring-applied fertilizer treatments (untreated control [UC], PL, and commercial fertilizer [CF]) assigned to subplots...
April 4, 2024: Journal of Environmental Quality
#10
JOURNAL ARTICLE
Zejun Lin, Zhenchao Zhou, Xinyi Shuai, Guangshu Zeng, Ruiqi Bao, Hong Chen
Conventional disinfection processes, such as chlorination and UV radiation, are ineffective in controling antibiotic-resistant bacteria, especially disinfection residual Enterobacteriaceae (DRE) encoding β-lactamases, some of which have been classified as "critical priority pathogens" by the World Health Organization. However, few studies have focused on the transferability, phenotype, and genetic characteristics of DRE-derived plasmids encoding β-lactamases, especially extended-spectrum β-lactamases and carbapenemases...
March 29, 2024: Water Research
#11
JOURNAL ARTICLE
Mehran Sardari, Meysam Manouchehrifar, Kamal Hasani, Nasrin Habibzadeh, Hadi Peeri Doghaheh, Taher Azimi, Mohsen Arzanlou
Beta-lactamase-producing Enterobacterales bacteria cause severe hard-to-treat infections. Currently, they are spreading beyond hospitals and becoming a serious global health concern. This study investigated the prevalence and molecular characterization of extended-spectrum β-lactamase and AmpC-type β-lactamase-producing Enterobacterales (ESBL-PE, AmpC-PE) in wastewater from livestock and poultry slaughterhouses in Ardabil, Iran. A total of 80 Enterobacterales bacteria belonging to 9 species were identified...
March 2024: Journal of Water and Health
#12
JOURNAL ARTICLE
Feng Tang, Changzhen Li, Ran Li, Lei Xi, Fang Wang, Juling Tian, Wanjun Luo
The urgent need for comprehensive and systematic analyses of Shigella as the key pathogen led us to meticulously explore the epidemiology and molecular attributes of Shigella isolates. Accordingly, we procured 24 isolates (10 from Xinjiang and 14 from Wuhan, China) and performed serotype identification and antimicrobial susceptibility testing. Resistance gene detection and homology analysis by polymerase chain reaction and pulsed-field gel electrophoresis (PFGE), respectively, were performed for genetic diversity analysis...
April 1, 2024: Foodborne Pathogens and Disease
#13
JOURNAL ARTICLE
Hokyung Song, Jung Sik Yoo, Tatsuya Unno
Extended-spectrum beta-lactamase (ESBL)-producing bacteria are resistant to most beta-lactams, including third-generation cephalosporins, limiting the treatment methods against the infections they cause. In this study, we performed whole genome sequencing of ESBL-producing E. coli to determine the mechanisms underlying the dissemination of antibiotic resistance genes. We analyzed 141 ESBL-producing isolates which had been collected from 16 veterinary clinics and 16 farms in South Korea. Long- and short-read sequencing platforms were used to obtain high-quality assemblies...
March 28, 2024: Science of the Total Environment
#14
JOURNAL ARTICLE
Maria Nieto-Rosado, Kirsty Sands, Edward A R Portal, Kathryn M Thomson, Maria J Carvalho, Jordan Mathias, Rebecca Milton, Calie Dyer, Chinenye Akpulu, Ian Boostrom, Patrick Hogan, Habiba Saif, Ana D Sanches Ferreira, Thomas Hender, Barbra Portal, Robert Andrews, W John Watkins, Rabaab Zahra, Haider Shirazi, Adil Muhammad, Syed Najeeb Ullah, Muhammad Hilal Jan, Shermeen Akif, Kenneth C Iregbu, Fatima Modibbo, Stella Uwaezuoke, Lamidi Audu, Chinago P Edwin, Ashiru H Yusuf, Adeola Adeleye, Aisha S Mukkadas, Jean Baptiste Mazarati, Aniceth Rucogoza, Lucie Gaju, Shaheen Mehtar, Andrew N H Bulabula, Andrew Whitelaw, Lauren Roberts, Grace Chan, Delayehu Bekele, Semaria Solomon, Mahlet Abayneh, Gesit Metaferia, Timothy R Walsh
Hospital surfaces can harbour bacterial pathogens, which may disseminate and cause nosocomial infections, contributing towards mortality in low- and middle-income countries (LMICs). During the BARNARDS study, hospital surfaces from neonatal wards were sampled to assess the degree of environmental surface and patient care equipment colonisation by Gram-negative bacteria (GNB) carrying antibiotic resistance genes (ARGs). Here, we perform PCR screening for extended-spectrum β-lactamases (blaCTX-M-15 ) and carbapenemases (blaNDM , blaOXA-48 -like and blaKPC ), MALDI-TOF MS identification of GNB carrying ARGs, and further analysis by whole genome sequencing of bacterial isolates...
March 29, 2024: Nature Communications
#15
JOURNAL ARTICLE
Natalia Maldonado, Inmaculada López-Hernández, Andrea García-Montaner, Luis Eduardo López-Cortés, Pedro María Martínez Pérez-Crespo, Pilar Retamar-Gentil, Adrián Sousa-Domínguez, Josune Goikoetxea, Ángeles Pulido-Navazo, Cristina Labayru-Echeverría, Clara Natera-Kindelán, Alfredo Jover-Sáenz, Alfonso Del Arco-Jiménez, Carlos Armiñanzas-Castillo, Ana Isabel Aller, Jonathan Fernández-Suárez, Teresa Marrodán-Ciordia, Lucía Boix-Palop, Alejandro Smithson-Amat, José Mª Reguera-Iglesias, Fátima Galán-Sánchez, Alberto Bahamonde, Juan Manuel Sánchez Calvo, Isabel Gea-Lázaro, Inés Pérez-Camacho, Armando Reyes-Bertos, Berta Becerril-Carral, Jesús Rodríguez-Baño, Álvaro Pascual
BACKGROUND: Escherichia coli is the most frequent cause of bloodstream infections (BSIs). About one-third of patients with BSIs due to E coli develop sepsis or shock. The objective of this study is to characterise the microbiological features of E coli blood isolates causing sepsis or septic shock to provide exploratory information for future diagnostic, preventive, or therapeutic interventions. METHODS: E coli blood isolates from a multicentre cross-sectional study of patients older than 14 years presenting with sepsis or septic shock (according to the Third International Consensus Definitions for Sepsis and Septic Shock criteria) from hospitals in Spain between Oct 4, 2016, and Oct 15, 2017, were studied by whole-genome sequencing...
March 25, 2024: The Lancet. Microbe
#16
JOURNAL ARTICLE
Milena Dropa, Jéssica Santiago Bispo da Silva, André Furugen César Andrade, Denis Hideki Nakasone, Marcos Paulo Vieira Cunha, Gesiane Ribeiro, Ronalda Silva de Araújo, Carlos Jesus Brandão, Barbara Ghiglione, Nilton Lincopan, Maria Inês Zanoli Sato, Terezinha Knöbl
The spread of antimicrobial resistance (AMR) through multiple reservoirs is a global concern. Wastewater is a critical AMR dissemination source, so this study aimed to assess the persistence of resistance genetic markers in wastewater using a culture-independent approach. Raw and treated wastewater samples (n = 121) from a wastewater treatment plant (WWTP), a human hospital, a veterinary hospital, and a pig farm were monthly collected and concentrated by filtration. DNA was extracted directly from filter membranes, and PCR was used in the qualitative search of 32 antimicrobial resistance genes (ARGs)...
March 28, 2024: Tropical Medicine & International Health
#17
REVIEW
Muttiah Barathan, Sook-Luan Ng, Yogeswaran Lokanathan, Min Hwei Ng, Jia Xian Law
This paper sheds light on the alarming issue of antibiotic resistance (ABR) in aquatic environments, exploring its detrimental effects on ecosystems and public health. It examines the multifaceted role of antibiotic use in aquaculture, agricultural runoff, and industrial waste in fostering the development and dissemination of resistant bacteria. The intricate interplay between various environmental factors, horizontal gene transfer, and bacterial extracellular vesicles (BEVs) in accelerating the spread of ABR is comprehensively discussed...
March 7, 2024: International Journal of Molecular Sciences
#18
JOURNAL ARTICLE
Jie Liu, Yue Hou, Linna Zhao, Gang Chen, Jialiang Chen, Qiang Zhao, Liyan Ye, Shenghui Cui, Chengbin Wang
BACKGROUND: With the recent evolution of multidrug-resistant strains, the genetic characteristics of foodborne Salmonella enterica serovar Enteritidis and clinical isolates have changed. ST11 is now the most common genotype associated with S . Enteritidis isolates. METHODS: A total of 83 strains of S . Enteritidis were collected at the General Hospital of the People's Liberation Army. Of these, 37 were from aseptic sites in patients, 11 were from the feces of patients with diarrhea, and the remaining 35 were of chicken-origin...
March 19, 2024: Frontiers in Bioscience (Landmark Edition)
#19
JOURNAL ARTICLE
Junling Cui, Yanbin Dong, Qiuru Chen, Chaojun Zhang, Kun He, Gongzheng Hu, Dandan He, Li Yuan
Extended-spectrum-β-lactamases (ESBLs)-producing Escherichia coli conferred resistance to most β-lactams, except for carbapenems. To date, the transmission mechanism of blaCTX-M , as the most common ESBLs subtype, in E. coli has received sustained attention around the worldwide, but the research on the pathogenicity of blaCTX-M -bearing E. coli is still scarce. The aims of this study were to discern the spread characteristics of ColV (encoding colicin V) plasmids in blaCTX-M -positive E. coli. The multi-drug resistance traits, phylogroups, and ColV plasmid profilings were screened in 76 blaCTX-M -positive E...
March 7, 2024: Poultry Science
#20
JOURNAL ARTICLE
Safir Ullah, Saeed Ul Hassan Khan, Muhammad Jamil Khan, Baharullah Khattak, Fozia Fozia, Ijaz Ahmad, Mohammad Ahmad Wadaan, Muhammad Farooq Khan, Almohannad Baabbad, Sagar M Goyal
INTRODUCTION: Raw milk may contain pathogenic microorganisms harmful to humans, e.g., multidrug-resistant Escherichia coli non-O157:H7, which can cause severe colitis, hemolytic uremia, and meningitis in children. No studies are available on the prevalence of Shiga toxin-producing E. coli (STEC O157:H7) in sick or healthy dairy animals in the Khyber Pakhtunkhwa Province of Pakistan. AIM: This study aimed to isolate, characterize, and detect antibiotic resistance in STEC non-O157:H7 from unpasteurized milk of dairy bovines in this province...
March 19, 2024: Tropical Medicine and Infectious Disease
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