Waleed Ak Tareen, Evelyn Saba, Usman Rashid, Adeel Sarfraz, Muhammad S Yousaf, Habib-Ur-Rehman, Hafiz F Rehman, Mansur Abdullah Sandhu
OBJECTIVE: In regenerative biology, the most commonly used cells are adipose tissue-derived mesenchymal stem cells (AD-MSCs). This is due to the abundance and easy accessibility of AD-MSCs. METHODS: In this study, canine AD-MSCs were harvested from different anatomical locations, i.e., subcutaneous (SC), omental (OM), and perirenal (PR). Various isolation techniques namely explants (TRT-I), collagenase-digestion (TRT-II), collagenase-digested explants (TRT-III), and trypsin-digested explants (TRT-IV) were used to segregate the MSCs to evaluate cell doubling time, viability, and adipogenic/osteogenic lineage differentiation potential...
2024: American Journal of Stem Cells